the genetic characterization for peach varieties tropical and dourad？o was realized by the rapd method, also comparing with dourado-1, tutu, maravilha, rubro-sol, flordaprince, aurora-1 and talism？ varieties, from iac "frutas de caro？o" germplasm active bank, maintained in núcleo de agronomia de cap？o bonito, using dna extracted of young leaves, with the purpose of establishing efficient markers in the variety identification. were obtained 31 markers from highly polimorphic primers opad10, opae04, opae07, opae09, opae11, opaj04 and opaj06, selected from 96 primers evaluated. the rapd markers used, indicated the relationship degree among varieties in an efficient way; except in varieties of free polinization origin. even among the varieties siblings as tutu and talism？ had certain genetic distance (0,29), showing that the rapd is capable to characterize kindred materials. the tropical and dourad？o peaches varieties were well characterized in relation to the parents and others varieties, with genetic distances varying fron 0,26 to 0,89.