the objective of this study was to evaluate the quality of percolation from three soil classes that received vinasse doses with different incubation times. the soils, classified as haplic nitosol, yellow argisol and carbic spodosol, were reproduced in twenty-seven 20 × 110 cm pvc columns (diameter × height) with horizons, thickness and densities similar to original soil. the soils were treated with vinasse doses equivalent to 0 (control), 350 and 700 m3 ha-1, and submitted to 30 and 60 days incubation. the collected effluents were analyzed for bod5, cod, ec, tds and ph. the results indicated that variations in the analyzed parameters, due to vinasse application in the soil, do not bring concerns in relation to environmental quality. despite different textures and consequently, different physiochemical properties, the comparison of percolates with natural vinasse data indicated that the soils presented high retention power, reducing the possibility of this waste causing pollution to the subsurface waters.