The ability to study bacteria at the single cell level has advanced our insights into microbial physiology and genetics in ways not attainable by studying large populations using more traditional culturing methods. To improve methods to characterize bacteria at the cellular level, we developed a new microfluidic platform that enables cells to be exposed to metabolites in a gradient of concentrations. By designing low-cost, three-dimensional devices with adhesive tapes and tailoring them for bact...

Electric Engineering  Biological Materials  Mycology  Biological Chemistry  Veterinary Medicine  Molecular Biology  Toxicology  Computer Engineering  Microbiology  Biochemistry  Agricultural Engineering  Biodiversity  Developmental Biology  Bioengineering  Environmental Sciences  Environmental Sciences  Biological Engineering  Biotechnology  Chemical Engineering & Technology  Hydrology  Genomics  Genetics  Food Science & Technology  Analytical Chemistry 

Doi:10.1021/acssensors.9b01031

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in exponential growth phase can be heterogeneous with respect to variables typically used to measure cell growth. Bacterial heterogeneity has important implications for how bacteria respond to environmental stresses, such as antibiotics. The phenomenon of antimicrobia...

Ecology  Developmental Biology  Agricultural Engineering  Environmental Sciences  Artificial Intelligence  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Diagnostics  Biological Materials  Electric Engineering  Genetic Engineering  Industrial Engineering  Computer Engineering  Health Policy  Biochemistry  Biodiversity  Bioengineering  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology  Infectious Diseases  Immunology  Internal Medicine  Metabolic Sciences  Medical Genetics  Allergy & Clinical Immunology  Zoology  Biological Chemistry  Applied Physics  Taxonomy  Medicinal Chemistry  Agricultural Science  Analytical Chemistry  Mycology  Public Health  Mathematical Biology  Marine Biology  Molecular Biology  Microbiology  Global Health  Parasitology  Plant Science  Translational Medicine  Soil Science  Synthetic Biology  Materials Engineering 

Doi:10.1016/j.ohx.2021.e00249

Population-scale and rapid testing for SARS-CoV-2 continues to be a priority for several parts of the world. We revisit the in vitro technology platforms for COVID-19 testing and diagnostics—molecular tests and rapid antigen tests, serology or antibody tests, and tests for the management of COVID-19 patients. Within each category of tests, we review the commercialized testing platforms, their analyzing systems, specimen collection protocols, testing methodologies, supply chain logistics, and rel...

Allergy & Clinical Immunology  Electrochemistry  Information Technologies  Medical Education  Biological Chemistry  Virology  Medicinal Chemistry  Developmental Biology  Environmental Sciences  Biological Engineering  Genomics  Genetics  Food Science & Technology  Marketing  Oncology  Technology And Innovation  Theoretical Chemistry  Diagnostics  Supply Chain Management  Biological Materials  Nanometer Materials  Drugs & Devices  Public Health  Organic Chemistry  Epidemiology  Sales Management  Molecular Biology  Health Policy  Microbiology  Global Health  Bioengineering  Physical Chemistry  Bioinformatics  Biotechnology  Functional Materials  Computational Biology  Cell Biology  Parasitology  Translational Medicine  Infectious Diseases  Women’s Health  Immunology  Respiratory Medicine  Materials Engineering  Internal Medicine  Metabolic Sciences  Medical Genetics 

Doi:10.3390/s21196581

The goal of the study was to ascertain sperm proteins as fertility markers in Angus bull sperm using proteomics and validating the markers through comparative sperm biology between Angus and Holstein bulls for which there are reliable fertility data available. We aimed to determine proteins differentially expressed in sperm from Angus bulls with different fertility phenotypes. Two-dimensional differential gel electrophoresis with mass-spectrometry, functional gene clusters, canonical pathways an...

Zoology  Computational Biology  Developmental Biology 

Doi:10.4308/hjb.27.2.116

Low nuclear maturation and fertilization rate is one obstacle in the in vitro embryo production which decrease embryo yield. This problem is presumable related with high production of reactive oxygen species (ROS) during maturation process. Glutathione (GSH) as an antioxidant is well known to overcome effect of ROS production. GSH synthesis in the cytosol part of the oocyte cytoplasm is influenced by cysteine availability. It is therefore, this research was conducted to evaluate the ability of c...

Anatomy & Physiology  Cell Biology  Developmental Biology 

Doi:10.4308/hjb.27.4.290

The methonolic extract of the leaves of Pisonia alba (PA) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, Antioxidant activity, Nitric oxide potential. The analyses revealed that the methanol extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compare...

Zoology  Virology  Ecology  Ecosystem Science  Conservation Biology  Developmental Biology  Evolutionary Studies  Entomology  Environmental Sciences  Animal Behavior  Agricultural Science  Histology  Genomics  Genetics  Food Science & Technology  Anthropology  Aquaculture, Fisheries & Fish Science  Biochemistry  Biodiversity  Bioengineering  Biogeography  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology 

Doi:10.5281/zenodo.1312689

Aim. Frequency of alien chromatin inheritance in hybrids from crosses of common wheat and introgressive lines determines whether transfer of alien chromatin controlling desirable trait into common wheat genome would be successful. The paper presents results of introgressive lines assessment for frequency of transfer of alien dominant trait of powdery mildew resistance to their progeny in the crossing with recurrent genotype Aurora, which was a recipient cultivar in the development of these lines...

Plant Science  Developmental Biology 

Doi:10.18523/2617-4529.2018.13-20

Many new facts and theories have appeared in physiology. The solution of complex physiological problems required collaboration of physiologists, pathologists, biochemists, biophysicists, mathematicians, and other specialists. That was possible due to the rapid development of new technologies as well as the robust foundation laid by the outstanding physiologists of the 19th century. This article describes the activities of outstanding Russian and Ukrainian physiologists of the 19th and the early...

Mathematical Biology  Parasitology  Biochemistry  Developmental Biology  Biophysics 

Doi:10.18523/2617-4529.2018.34-42

A study was conducted to determinate the effects of Tiotriasolin, Qudesan and the precursors of ubiquinone synthesis (EPM-Mg: alpha-tocopherol acetate), 4-hydroxybenzoic acid, methionine, Mg2 ions) on the free radical processes and activity of some Krebs cycle dehydrogenases in rat heart and liver tissues under conditions of doxorubicin-induced cardiomyopathy. Administration of doxorubicin intensified the processes of lipid and protein oxidation and suppressed antioxidant enzymes activity in li...

Zoology  Biochemistry  Developmental Biology 

Doi:10.18523/2617-4529.2018.28-33

Aim. The purpose of the work is to identify and study particular biological characteristics of the genus Fusarium isolates, received from the Triticum aestivum L grain. Methods. In this paper, the cultural and morphological properties of investigating isolates of micellar fungi are studied in order to identify them to species further. For the detailed characteristics of the isolates, the speed of radial growth of the mycelium and the intensity of spore formation are also determined. Results. Bas...

Plant Science  Developmental Biology 

Doi:10.18523/2617-4529.2018.21-27