【Objective】 The purpose of this study was to investigate the toxic effect of perfuorooctanoic aid(PFOA) on mouse oocytes.【Method】 One hundred and sixty 6-week-old Kunming female mice with similar body weight were divided randomly into four groups, with five replicates in each group and eight mice in each replicate. After 7 days of adaptation, the mice were fed with different doses of PFOA for 14 days. The daily doses for control, low, medium, and high dose groups were 0, 5, 10 and 20 mg/kg respe...

Agricultural Science  Zoology  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202101028

【Objective】 Mulberry bacterial wilt is a seriously harmful bacterial disease caused by Ralstonia solanacearum. Therefore, it is of great significance to establish a rapid and sensitive detection method for R. solanacearum to effectively control mulberry bacterial wilt. 【Method】 Pectate lyase gene of R. solanacearum was used as the target. Based on the primer design principle of isothermal multiple self-matching-initiated amplification (IMSA), combining the loop-mediated isothermal amplification ...

Agricultural Science  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202203030

【Objective】 The metabolites of Epicoccum sorghinum L28 from mangrove and their antifungal activities were studied. 【Method】 The metabolites were isolated and purified by column chromatography, and their structures were identified by spectral technique. The minimum inhibitory concentration (MIC) of the metabolites against Fusarium graminearum and F. oxysporum were tested by the double dilution method. 【Result】 Ten compounds were isolated and identified as 7-hydroxy-2, 5-dimethyltrichroprimorone (...

Agricultural Science  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202112017

【Objective】 The changes of water loss status, soluble sugar content and relative enzyme gene expression in related glyco metabolism signal pathway of cassava stem under different storage temperature and time were studied, and the correlation between trehalose content and water loss rate of cassava stem was analyzed.【Method】 The stem of ‘cassava 60444’ was used as the experimental material. The stem was stored under low temperature of (20±2)℃ and normal temperature of (36±2)℃ for 0–30 d after har...

Agricultural Science  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202109030

【Objective】 To investigate the effects of astragaloside IV (AS-IV) and astragalus polysaccharides (APS) of astragalus extract on the lipopolysaccharides (LPS)-induced inflammation of bovine mammary epithelial cells (BMECs) and the effect on the expression of β-casein in inflammatory cells. 【Method】 APS and AS-IV were used to intervene LPS-induced BMECs to detect the changes of cell inflammatory factors, oxidative factors, apoptosis factors and β-casein. 【Result】 CCK-8 screening found the concent...

Agricultural Science  Zoology  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202106034

【Objective】 The existing position and extraction method of antifungal substance produced by Chaetomium subaffine strain LB-1 were studied in order to lay a foundation for the development of the strain LB-1 to control plant diseases. 【Method】 With Botrytis cinerea and Exserohilum turcicum as test plant pathogens, the sealed plate assay was used to detect whether the strain LB-1 could produce volatile antifungal substance. The hyphae ultrasonic breaking and liquid culture methods were used to dete...

Agricultural Science  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202204019

【Objective】 To clarify the pathogen causing Trichosanthes kirilowii fruit rot and screen effective fungicides for controlling the disease. 【Method】 Samples of rot fruit from T. kirilowii plants were collected from Dabie Mountain Areas, Anhui Province in 2019—2020. A total of 35 representative isolates were obtained by tissue isolation. The pathogenicity was verified according to the Koch’s postulate, and the causal agents were identified based on the morphological characteristics and multilocus ...

Agricultural Science  Cell Biology  Plant Science  Agronomy 

Doi:10.7671/j.issn.1001-411X.202202006

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in exponential growth phase can be heterogeneous with respect to variables typically used to measure cell growth. Bacterial heterogeneity has important implications for how bacteria respond to environmental stresses, such as antibiotics. The phenomenon of antimicrobia...

Ecology  Developmental Biology  Agricultural Engineering  Environmental Sciences  Artificial Intelligence  Biological Engineering  Genomics  Chemical Engineering & Technology  Genetics  Food Science & Technology  Diagnostics  Biological Materials  Electric Engineering  Genetic Engineering  Industrial Engineering  Computer Engineering  Health Policy  Biochemistry  Biodiversity  Bioengineering  Bioinformatics  Biophysics  Biotechnology  Computational Biology  Cell Biology  Infectious Diseases  Immunology  Internal Medicine  Metabolic Sciences  Medical Genetics  Allergy & Clinical Immunology  Zoology  Biological Chemistry  Applied Physics  Taxonomy  Medicinal Chemistry  Agricultural Science  Analytical Chemistry  Mycology  Public Health  Mathematical Biology  Marine Biology  Molecular Biology  Microbiology  Global Health  Parasitology  Plant Science  Translational Medicine  Soil Science  Synthetic Biology  Materials Engineering 

Doi:10.1016/j.ohx.2021.e00249

Population-scale and rapid testing for SARS-CoV-2 continues to be a priority for several parts of the world. We revisit the in vitro technology platforms for COVID-19 testing and diagnostics—molecular tests and rapid antigen tests, serology or antibody tests, and tests for the management of COVID-19 patients. Within each category of tests, we review the commercialized testing platforms, their analyzing systems, specimen collection protocols, testing methodologies, supply chain logistics, and rel...

Allergy & Clinical Immunology  Electrochemistry  Information Technologies  Medical Education  Biological Chemistry  Virology  Medicinal Chemistry  Developmental Biology  Environmental Sciences  Biological Engineering  Genomics  Genetics  Food Science & Technology  Marketing  Oncology  Technology And Innovation  Theoretical Chemistry  Diagnostics  Supply Chain Management  Biological Materials  Nanometer Materials  Drugs & Devices  Public Health  Organic Chemistry  Epidemiology  Sales Management  Molecular Biology  Health Policy  Microbiology  Global Health  Bioengineering  Physical Chemistry  Bioinformatics  Biotechnology  Functional Materials  Computational Biology  Cell Biology  Parasitology  Translational Medicine  Infectious Diseases  Women’s Health  Immunology  Respiratory Medicine  Materials Engineering  Internal Medicine  Metabolic Sciences  Medical Genetics 

Doi:10.3390/s21196581

The animal model response against genetically modifed product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into fve treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pellete...

Animal Behavior  Histology  Genetics  Cell Biology 

Doi:10.4308/hjb.26.1.1