Escherichia coli, a Gram-negative bacterium, is commonly associated community and hospital-acquired infections, with significant antibiotic resistance implications for public health. This study aimed to detect the blaVIM gene in E. coli strains isolated from the Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle (CHUP-CDG) in Ouagadougou, Burkina Faso. E. coli strains were isolated from various biological samples (urine, pus, blood, stool, and cerebrospinal fluid) from 2009 to 2013 at CHUP-CDG. Antibiotic susceptibility testing for cefotaxime, ceftazidime, and imipenem was performed using the disc diffusion method on Mueller-Hinton agar. Classical PCR was used to identify the blaVIM gene. The susceptibility test showed high resistance of strains to third generation cephalosporins. The resistance rate was 82.86% (29/35), 80.00% (28/35) and 11.42% (4/35) for cefotaxime, ceftriaxone, and imipenem, respectively. Analysis of PCR products revealed that 11.42% (4/35) of strains harbored the blaVIM gene. Significantly, 75.00% (3/4) of strains with the blaVIM gene were isolated from urine samples. The present study demonstrated the presence of the blaVIM gene in E. coli resistant strains to β-lactams at CHUP-CDG. Our results suggest the presence of other resistance genes in view of the low rate of blaVIM found in resistant strains. Surveillance measures are necessary to prevent the spread of these resistant strains.
Cite this paper
Ouattara, A. K. , Ouédraogo, B. , Tiemtoré, R. Y. W. , Dabiré, A. M. , Sougué, S. and Simporé, J. (2023). Detection of blaVIM Gene in β-Lactam Resistant E. coli Isolated from Clinical Samples in Ouagadougou, Burkina Faso. Open Access Library Journal, 10, e534. doi: http://dx.doi.org/10.4236/oalib.1110534.
Shin, H., Kim, Y., Unno, T. and Hur, H.G. (2023) Prevalence and Characterization of CRISPR Locus 2.1 Spacers in Escherichia coli Isolates Obtained from Feces of Animals and Humans. Microbiology Spectrum, 11, e0493422.
https://doi.org/10.1128/spectrum.04934-22
Weerdenburg, E., Davies, T., Morrow, B., Zomer, A.L., Hermans, P., Go, O., Spiessens, B., van den Hoven, T., van Geet, G., Aitabi, M., DebRoy, C., Dudley, E.G., Bonten, M., Poolman, J. and Geurtsen, J. (2022) Global Distribution of O Serotypes and Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Collected from the Blood of Patients with Bacteremia across Multiple Surveillance Studies. Clinical Infectious Diseases, 76, e1236-e1243. https://doi.org/10.1093/cid/ciac421
Varghese, A., Saleena, U.V., Bhat, G., et al. (2023) Comparison of Genetic Factors of Escherichia coli in Patients with Urosepsis and Urinary Tract Infections. A Systematic Review. Reviews and Research in Medical Microbiology.
Yair, Y., Michaux, C., Biran, D., Bernhard, J., Vogel, J., Barquist, L. and Ron, E.Z. (2022) Cellular RNA Targets of Cold Shock Proteins CspC and CspE and Their Importance for Serum Resistance in Septicemic Escherichia coli. mSystems, 7, e00086-22. https://doi.org/10.1128/msystems.00086-22
Gemeinder, J.L.P., Barros, N.R., Pegorin, G.S., Singulani, J.L., Borges, F.A., Arco, M., Giannini, M., Almeida, A.M.F., Salvador, S.L.S. and Herculano, R.D. (2021) Gentamicin Encapsulated within a Biopolymer for the Treatment of Staphylococcus aureus and Escherichia coli Infected Skin Ulcers. Journal of Biomaterials Science, Polymer Edition, 32, 93-111. https://doi.org/10.1080/09205063.2020.1817667
Mueller, T. and ystergren, P.-O. (2016) The Correlation between Regulatory Conditions and Antibiotic Consumption within the WHO European Region. Health Policy, 120, 882-889. https://doi.org/10.1016/j.healthpol.2016.07.004
Wu, W., Feng, Y., Tang, G., Qiao, F., McNally, A. and Zong, Z. (2019) NDM Metallo-β-Lactamases and Their Bacterial Producers in Health Care Settings. Clinical Microbiology Reviews, 32, e00115-18. https://doi.org/10.1128/CMR.00115-18
Shams, S., Hashemi, A., Esmkhani, M., Kermani, S., Shams, E. and Piccirillo, A. (2018) Imipenem Resistance in Clinical Escherichia coli from Qom, Iran. BMC Research Notes, 11, Article No. 314. https://doi.org/10.1186/s13104-018-3406-6
Metuor Dabire, A., Zongo, K.J., Zeba, B., Moussawi, J., Baucher, M. and El Jaziri, M. (2013) Resistances to the Oxyimino-Cephalosporins by Ctx-M-15 Producing Klebsiella Isolated From the Urines Samples of Patients in the University Hospital Complex Paediatric Charles De Gaulle (CHUP-CDG) of Ouagadougou in Burkina Faso. Journal of Asian Scientific Research, 3, 882-890.
https://archive.aessweb.com/index.php/5003/article/view/3540
Metuor Dabire, A., Zongo, K.J., Zeba, B., Traoré/Ouedraogo, R., Moussawi, J., Baucher, M. and El Jaziri, M. (2014) First Detection of SHV-Type Extended Spectrum β-Lactamases in the University Hospital Complex Paediatric Charles De Gaulle (CHUP-CDG) of Ouagadougou in Burkina Faso. Journal of Asian Scientific Research, 4, 214-221.
https://archive.aessweb.com/index.php/5003/article/view/3629
Mètuor, D.A., Tiemtoré, R.Y.W.-K., Bangré, Y.A., Zohoncon, T.M., Sougué, S., Zongo, J.K. and Simporé, J. (2019) Detection of Multidrug-Resistant Enterobacteria Simultaneously Producing Extended-Spectrum-Lactamases of the PER and GES Types Isolated at Saint Camille Hospital Center, Ouagadougou, Burkina Faso. African Journal of Microbiology Research, 13, 414-420.
https://doi.org/10.5897/AJMR2019.9147
Catalano, A., Iacopetta, D., Ceramella, J., Scumaci, D., Giuzio, F., Saturnino, C., Aquaro, S., Rosano, C. and Sinicropi, M.S. (2022) Multidrug Resistance (MDR): A Widespread Phenomenon in Pharmacological Therapies. Molecules, 27, Article No. 616. https://doi.org/10.3390/molecules27030616
Dembélé, R., Soulama, I., Kaboré, W.A.D., Konaté, A., Kagambèga, A., N'Golo, D.C., Traoré, O., Seck, A., Traoré, A.S. and Guessennd, N. (2021) Molecular Characterization of Carbapenemase-Producing Enterobacterales in Children with Diarrhea in Rural Burkina Faso. Journal of Drug Delivery and Therapeutics, 11, 84-92.
https://doi.org/10.22270/jddt.v11i1.4513
Bonko, M.D.A., Tahita, M.C., Kiemde, F., Lompo, P., Yougbaré, S., Some, A.M., Tinto, H., Mens, P.F., Menting, S. and Schallig, H. (2021) Antibiotic Susceptibility Profile of Bacterial Isolates from Febrile Children under 5 Years of Age in Nanoro, Burkina Faso. Tropical Medicine & International Health, 26, 1220-1230.
https://doi.org/10.1111/tmi.13644
Khalifa, S.M., El-Aziz, A.M.A., Hassan, R. and Abdelmegeed, E.S. (2021) β-Lactam Resistance Associated with β-Lactamase Production and Porin Alteration in Clinical Isolates of E. coli and K. pneumoniae. PLOS ONE, 16, e0251594.
https://doi.org/10.1371/journal.pone.0251594
Toudji, A.G., Djeri, B., Karou, S.D., Tigossou, S., Ameyapoh, Y. and De Souza, C. (2017) Prévalence des souches d’entérobactéries productrices de bêta-lactamases à spectre élargi isolées au Togo et de leur sensibilité aux antibiotiques. International Journal of Biological and Chemical Sciences, 11, 1165-1177.
https://doi.org/10.4314/ijbcs.v11i3.19
Zorgani, A., Almagatef, A., Sufya, N., Bashein, A. and Tubbal, A. (2017) Detection of CTX-M-15 among Uropathogenic Escherichia coli Isolated from Five Major Hospitals in Tripoli, Libya. Oman Medical Journal, 32, 322-327.
https://doi.org/10.5001/omj.2017.61
Diagbouga, S., Salah, F., Sadji, A., Dabire, A., Nadembega, C., Kere, A., Soubeiga, S., Ouattar, A., Zohoncon, T., Belemgnegre, M., Karou, S. and Simpore, J. (2016) Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca. Journal of Clinical and Diagnostic Research, 4, Article ID: 000129.
Jain, P., Bepari, A.K., Sen, P.K., Rafe, T., Imtiaz, R., Hossain, M. and Reza, H.M. (2021) High Prevalence of Multiple Antibiotic Resistance in Clinical E. coli Isolates from Bangladesh and Prediction of Molecular Resistance Determinants Using WGS of an XDR Isolate. Scientific Reports, 11, Article No. 22859.
https://doi.org/10.5001/omj.2017.61
Tiemtoré, R.Y., Mètuor-Dabiré, A., Zohoncon, T.M., Bangré, Y.A., Sougue, S., Zongo, J. and Simpore, J. (2019) First Detection of PE-Type Extended-Spectrum β-Lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso. International Journal of Biochemistry, Biophysics & Molecular Biology, 4, 7-12.
https://doi.org/10.11648/j.ijbbmb.20190401.12
Tiemtoré, R.Y.W., Mètuor Dabiré, A., Ouermi, D., Sougué, S., Benao, S. and Simporé, J. (2022) Isolation and Identification of Escherichia coli and Klebsiella pneumoniae Strains Resistant to the Oxyimino-Cephalosporins and the Monobactam by Production of GES Type Extended Spectrum Bêta-Lactamase (ESBL) at Saint Camille Hospital Center in Ouagadougou, Burkina Faso. Infection and Drug Resistance, 15, 3191-3204. https://doi.org/10.2147/IDR.S360945
Moumouni, A., Diagbouga, S., Nadembèga, C., Metuor Dabire, A., Soubeiga, S.T., Ouattara, A.K., Zohoncon, T., Djigma, F., Langendorf, C. and Simpore, J. (2017) Quinolone Resistance (qnr) Genes in Fecal Carriage of Extended Spectrum Beta-Lactamases Producing Enterobacteria Isolated from Children in Niger. Current Research in Microbiology and Biotechnology, 5, 953-957.
http://crmb.aizeonpublishers.net/content/2017/1/crmb953-957.pdf
Salah, F., Diagbouga, S., Dabire, A.M., Sadji, A., Nadembega, C. and Moumouni, A. (2016) First Detection of Resistance Genes Encoding Extended Spectrum β-Lactamase Producing Escherichia coli at Lomé, Togo. Archives of Clinical Microbiology, 7, 32.
Salah, F.D., Soubeiga, S.T., Ouattara, A.K., Sadji, A.Y., Metuor-Dabire, A., Obiri-Yeboah, D., Banla-Kere, A., Karou, S. and Simpore, J. (2019) Distribution of Quinolone Resistance Gene (QNR) in ESBL-Producing Escherichia coli and Klebsiella spp. in Lomé, Togo. Antimicrobial Resistance & Infection Control, 8, Article No. 104. https://doi.org/10.1186/s13756-019-0552-0
Nmema, E. and Ologun, C. (2019) Detection of Blavim Genes in Clinical Isolates of Carbapenem-Resistant, Metallo-β-Lactamase Producing Pseudomonas aeruginosa. Annals of Biomedical Sciences, 18, 147-156.
https://www.ajol.info/index.php/abs/article/view/188575
Nahid, F., Khan, A.A., Rehman, S. and Zahra, R. (2013) Prevalence of Metallo-β-Lactamase NDM-1-Producing Multi-Drug Resistant Bacteria at Two Pakistani Hospitals and Implications for Public Health. Journal of Infection and Public Health, 6, 487-493. https://doi.org/10.1016/j.jiph.2013.06.006
