Hemolysis of biological samples has long been considered to impact the results of conventional biochemical examinations for various reasons. For some years now, suppliers of biochemistry automats and reagents have been providing thresholds or indexes for hemolysis, icterus and lactescence beyond which the results are no longer reliable. Hemolysis can interfere with assays performed in the laboratory. These interferences are due to the release in serum or plasma of constituents present in red blood cells. The present work aims to determine the rate of hemolyzed samples from the different departments of the Mohammed VI University Hospital of Oujda (Morocco) and to propose practical approaches to improve the accuracy and reliability of biochemical results.
Cite this paper
Farih, S. , Rahhab, O. , Nassiri, O. , Belmahi, S. , Benhamza, N. , Sebbar, E. and Choukri, M. (2022). The Rate of Hemolysis in Samples Taken at the Mohammed VI University Hospital of Oujda (Morocco). Open Access Library Journal, 9, e8687. doi: http://dx.doi.org/10.4236/oalib.1108687.
Lippi, G., Luca Salvagno, G., Blanckaert, N., Giavarina, D., Green, S., Kitchen, S., et al. (2009) Multicenter Evaluation of the Hemolysis Index in Automated Clinical Chemistry Systems. Clinical Chemistry and Laboratory Medicine, 47, 934-939.
https://doi.org/10.1515/CCLM.2009.218
Zumoffen, C., Gerrard, G., Ensinck, A. and D’Arrigo, M. (2008) Hemolysis Interference in Routine Clinical Chemistry Assays. M154. Clinical Chemistry and Laboratory Medicine, 46, S307.
Simundic, A.-M., Topic, E., Nikolac, N. and Lippi, G. (2010) Hemolysis Detection and Management of Hemolyzed Specimens. Biochemia Medica, 20, 154-159.
https://doi.org/10.11613/BM.2010.018
Glick, M.R., Ryder, K.W., Glick, S.J. and Woods, J.R. (1989) Unreliable Visual Estimation of the Incidence and Amount of Turbidity, Hemolysis, and Icterus in Serum from Hospitalized Patients. Clinical Chemistry, 35, 837-839.
https://doi.org/10.1093/clinchem/35.5.837
Atakan Erkal, F., Aykal, G., Yalçinkaya, H.M., Aksoy, N. and Özdemir, M. (2019) The Effect of Automated Hemolysis Index Measurement on Sample and Test Rejection Rates. Turkish Journal of Biochemistry, 44, 630-634.
https://doi.org/10.1515/tjb-2018-0462
Benlakehal, M., Le Bricon, T., Feugeas, J.P. and Bousquet, B. (2000) Influence de l’hémolyse sur le dosage et l’électrophorèse des protéines sériques. Annales de Biologie Clinique, 58, 367-371.
Carraro, P., Servidio, G. and Plebani, M. (2000) Hemolyzed Specimens: A Reason for Rejection or a clinical challenge. Clinical Chemistry, 46, 306-307.
https://doi.org/10.1093/clinchem/46.2.306
Sakota, R., Lodi, C.A., Sconziano, S.A., Beck, W. and Bosch, J.P. (2015) In Vitro Comparative Assessment of Mechanical Blood Damage Induced by Different Hemodialysis Treatments. Artificial Organs, 39, 1015-1023.
https://doi.org/10.1111/aor.12499
Nand, S., Bansal, V.K., Kozeny, G., Vertuno, L., Remlinger, K.A. and Jordan, J.V. (1985) Red Cell Fragmentation Syndrome with the Use of Subclavian Hemodialysis Catheters. Archives of Internal Medicine, 145, 1421-1423.
https://doi.org/10.1001/archinte.1985.00360080095013
Söderberg, J., Jonsson, P.A., Wallin, O., Grankvist, K. and Hultdin, J. (2009) Haemolysis Index—An Estimate of Preanalytical Quality in Primary Health Care. Clinical Chemistry and Laboratory Medicine, 47, 940-944.
https://doi.org/10.1515/CCLM.2009.227
Grant, M.S. (2003) The Effect of Blood Drawing Techniques and Equipment on the Hemolysis of ED Laboratory Blood Samples. Journal of Emergency Nursing, 29, 116-121. https://doi.org/10.1067/men.2003.66
Burns, E.R. and Yoshikawa, N. (2002) Hemolysis in Serum Samples Drawn by Emergency Department Personnel versus Laboratory Phlebotomists. Laboratory Medicine, 33, 378-380. https://doi.org/10.1309/PGM4-4F8L-2P1M-LKPB
Tanabe, P., Kyriacou, D.N., Garland, F. and Kyriacou, D. (2003) Factors Affecting the Risk of Blood Bank Specimen Hemolysis. Academic Emergency Medicine, 10, 897-900. https://doi.org/10.1197/aemj.10.8.897
Pretlow, L., Gandy, T., Leibach, E.K., Russell, B. and Kraj, B. (2008) A Quality Improvement Cycle: Hemolyzed Specimens in the Emergency Department. Clinical Laboratory Science: Journal of the American Society for Medical Technology, 21, 219-224.