All Title Author
Keywords Abstract


Effects of Different Inducer on the Accumulation of Essential Oil from Endophytic Fungi of Cinnamomum longepaniculatum

DOI: 10.4236/oalib.1105337, PP. 1-12

Subject Areas: Biochemistry, Biotechnology

Keywords: C. longepaniculatum, Endophytic Fungus, Single Factor, Orthogonal Test

Full-Text   Cite this paper   Add to My Lib

Abstract

The aim of this study was to investigate the effect of different inducer on the accumulation of essential oil in C. longepaniculatum endophytic fungus. The effect of the essential oil accumulation in the C. longepaniculatum fungus was studied by adding different inducer with an C. longepaniculatum endophytic fungus (serial number 2J1) with the ability of producing essential oil. The results show that the accumulation of essential oil can be greatly promoted by adding CaSO4 1.5 mg/L, H2O2 9 mmol/L, SA 15 mg/L and MnSO4 3 mmol/L in the PDB medium; compared with CK in the same condition increased 75.56%, 47.18%, 38.35%, 53.95% respectively; reached 0.0934 mg/L, 0.0783 mmol/L, 0.0736 mmol/L, 0.0819 mg/L; through the orthogonal experiment, the combinations 2.0 mg/L CaSO4, 12 mmol/L H2O2, 5 mg/L SA and 7 mmol/L MnSO4 were significantly different from other combinations; the yield reached 0.1847 mg/L and increased by 247.18%, indicating that adding inducer was an effective way to promote the accumulation of essential oil of endophytic fungi. This provides a reference for the artificial regulation of the secondary metabolites of C. longepaniculatum and the enhancement of subsequent microorganisms.

Cite this paper

Yan, K. , Yuan, P. and Wei, Q. (2019). Effects of Different Inducer on the Accumulation of Essential Oil from Endophytic Fungi of Cinnamomum longepaniculatum. Open Access Library Journal, 6, e5337. doi: http://dx.doi.org/10.4236/oalib.1105337.

References

[1]  Tan, R. and Zou, W. (2001) Endophytes: A Rich Source of Functional Metabolites. Natural Product Reports, 18, 448-459. https://doi.org/10.1039/b100918o
[2]  Hussain, H. and Krohn, K. (2007) Bioactive Chemical Constituents of Two Endo-phytic Fungi. Biochemical Systematics and Ecology, 35, 898-900.
https://doi.org/10.1016/j.bse.2007.04.011
[3]  Kuan, Y., Fang, C., Qin, W., et al. (2017) Effects of Endophytic Fungi on the Accumulation and Physiological and Biochemical Characteristics of Essential Oil from C. longepaniculatum. Biotechnology Bulletin, 33, 138-143.
[4]  Ma, X.W. (2016) Transcriptome Analysis of Stress and Stress of CkNF-YB1 and CkEDS1 in Cynanchum Komarovii.
[5]  Liu, J., Ding, G., Fang, L., et al. (2014) Study on Secondary Metabolites of Endophytic Fungus Penicillium polonicum. Chinese Journal of Traditional Chinese Medicine, 39, 3974-3977.
[6]  Eilert, U. (1987) Elicitation: Methodology and Aspects of Application. In: Constabel, F. and Vasil, I.K., Eds., Cell Culture and Somatic Cell Genetics of Plants, Vol. 4, Academic Press, New York, 153.
[7]  Yan, L., Tang, C. and Yang, S. (2015) Optimization of Paclitaxel Fermentation System for Aspergillus fumigatus TMS-26 by Precursors and Elicitors and Fermentation Conditions. Journal of Fungal Materials, 34, 1165-1175.
[8]  Song, Q., Huang, Y., Yang, H., et al. (2012) Optimization of Fermentation Conditions for Antibiotic Production by Actinomycetes YJ1 Strain against Sclerotinia sclerotiorum. Agricultural Sciences, 4, 95.
[9]  Ruiz-sanche, Z.J., Flores-Bustamantezr, Dendooven, L., et al. (2010) A Comparative Study of Taxol Production in Liquid and Solid-State Fermentation with Nigrospora sp. a Fungus Isolated from Taxus Globosa. Applied Microbiology, 109, 2144-2150.
[10]  Wu, X. (2013) Effects of Seed Extracts of Peach Blossom on Its Endophytic Active Compounds. Biotechnology Bulletin, No. 10, 93-97.
[11]  Wang, T., You, L., Huang, N.Y., et al. (2009) Antifungal Activities against Phytopathogens and Diversity of Endophytic Fungi Isolated from C. longepaniculatum. Jiangsu Journal of Agricultural Sciences, 1, 98-101.
[12]  You, L., Wang, T., Li, L., et al. (2009) Analyses on Volatile Organic Compound of 78 Endophytic Fungi Isolated from C. longepaniculatum. Journal of Northwest Agriculture and Forestry University, Natural Science Edition, 37, 193-198.
[13]  Tan, Y., Lu, H., Li, Q., et al. (2015) Effects of Camphor Oil on Active Compounds in Endophytic Fungi of C. longepaniculatum. Natural Product Research and Development, 27, 1070-1075.
[14]  Wang, T., Wei, S. and Wei, Q. (2007) Diversity of Endophytic Fungi in C. longepaniculatum Leaves. Journal of Yunnan University (Natural Science Edition), 29, 300-302.
[15]  Sun, T., Zhao, C. and Jin, F. (2002) Effects of Several Inorganic Ions on Physiological Metabolism of S. cerevisiae and Mechanism of Acid Production in Fermentation Process. Journal of Dalian Institute of Light Industry, No. 1, 29-32.
[16]  Jeon, B.K., Kwon, K., Kang, J.L., et al. (2015) Csk-Induced Phosphorylation of Src at Tyrosine 530 Is Essential for H2O2-Mediated Suppression of ERK1/2 in Human Umbilical Vein Endothelial Cells. Scientific Reports, 5, 1-15.
https://doi.org/10.1038/srep12725
[17]  Cocetta, G., Rossoni, M., Gardana, C., et al. (2015) Methyl Jasmonate Affects Phenolic Metabolism and Gene Expression in Blueberry (Vaccinium corymbosum). Physiologia Plantarum, 153, 269-283. https://doi.org/10.1111/ppl.12243
[18]  Cao, H., Nuruzzarnan, M., Xiu, H., et al. (2015) Transcriptome Analysis of Methyl Jasmonate-Elicited Panax Ginseng Adventitious Roots to Discover Putative Ginsenoside Biosynthesis and Transport Genes. International Journal of Molecular Sciences, 16, 3035-3057.

Full-Text


comments powered by Disqus