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Effect of Cadmium Repartition on Nitrogen Metabolism in Tobacco Seedlings

DOI: 10.4236/oalib.1104000, PP. 1-14

Subject Areas: Environmental Sciences

Keywords: Cadmium, Nitrate Reductase, Glutamate Dehydrogenase, Nitrogen Metabolism

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Abstract

Thirty-day-old tobacco seedlings (Nicotiana tabaccum, Bureley v) were subjected during one week to increasing cadmium (Cd) concentrations (0, 10, 20, 50 and 100 μM CdCl2). Increasing Cd stress led to a gradual decrease of dry weight (DW) production, water and nitrate contents. More than the half of Cd accumulated per plant was sequestered in the oldest leaf stage (S1 leaves). Leaves from S1 were the least affected by Cd stress. The activities of nitrate reductase (NR, EC 1.6.1.6), nitrite reductase (NiR, EC 1.7.7.1) were the least reduced in S1 leaves despite of the high presence of Cd ions. At 100 μM Cd, glutamine synthetase activity (GS, EC 6.3.1.2) from S1 leaves rose to become 2 times more important than control. Western Blot analysis showed that S1 GS activity induction was correlated to the GS1 and GS2 protein accumulation. Young leaves (S3 leaves) were more affected by Cd stress than old leaves. The GS activity reduction in S3 leaves was correlated to GS2 protein decrease detected by western-blot analysis. So, tobacco plant accumulated Cd ions in old leaves (S1 leaves) to protect younger leaves which are more sensitive to Cd effects. Leaves from S1 are a target organ to verify an eventual soil contamination per cadmium. This leaves may evolve adaptive process to partially inactivate Cd ions and maintain stable rate of nitrogen metabolism.

Cite this paper

Dguimi, H. M. , Alshehri, K. , Zaghdoud, C. , Albaggar, A. K. and Debouba, M. (2019). Effect of Cadmium Repartition on Nitrogen Metabolism in Tobacco Seedlings. Open Access Library Journal, 6, e4000. doi: http://dx.doi.org/10.4236/oalib.1104000.

References

[1]  Di Toppi, L. and Gabbrielli, R. (1999) Response to Cadmium in Higher Plants. Environmental and Experimental Botany, 41, 105-130.
https://doi.org/10.1016/S0098-8472(98)00058-6
[2]  McLaughlin, M.J. and Singh, B.R. (1999) Cadmium in Soils and Plants. Kluwer Academic Publishers, Dordrecht, 1-9. https://doi.org/10.1007/978-94-011-4473-5
[3]  Fediuc, E. and Erdei, L. (2002) Physiological and Biochemical Aspects of Cadmium Toxicity and Protective Mechanisms Induced in Phragmites australis and Typha latifolia. Journal of Plant Physiology, 159, 265-271.
https://doi.org/10.1078/0176-1617-00639
[4]  Wagner, G.J. and Trotter, M.M. (1982) Inducible Cadmium Binding Complexes of Cabbage and Tobacco. Plant Physiology, 69, 804-809.
https://doi.org/10.1104/pp.69.4.804
[5]  Wahid, A., Ghani, A., Ali, I. and Ashraf, M.Y. (2007) Effects of Cadmium on Carbon and Nitrogen Assimilation in Shoots of Mungbean [Vigna radiata (L.) Wilczek Seedlings. Journal of Agronomy & Crop Science, 193, 357-365.
https://doi.org/10.1111/j.1439-037X.2007.00270.x
[6]  Chaffei, C., Pageau, K., Suzuki, A., Gouia, H., Ghorbel, M.H. and Masclaux-Daubresse, C. (2004) Cadmium Toxicity Induced Changes in Nitrogen Management in Lycopersicon esculentum Leading to a Metabolic Safeguard through an Amino Acid Storage Strategy. Plant Cell Physiology, 45, 1681-1693.
https://doi.org/10.1093/pcp/pch192
[7]  Wang, L., Zhou, Q., Ding, L. and Sun, Y. (2008) Effect of Cadmium Toxicity on Nitrogen Metabolism in Leaves of Solanum nigrum L. as a Newly Found Cadmium Hyperaccumulator. Journal of Hazardous Materials, 154, 818-825.
https://doi.org/10.1016/j.jhazmat.2007.10.097
[8]  Maaroufi, H., Debouba, M., Ghorbel, M.H. and Gouia, H. (2009) Tissue-Specific Cadmium Accumulation and Its Effects on Nitrogen Metabolism in Tobacco (Nicotiana tabaccum, Bureley v. Fb9). Comptes Rendue de Biologie, 332, 58-68.
https://doi.org/10.1016/j.crvi.2008.08.021
[9]  Romero-Puertas, M.C., McCarthy, I., Gómez, M., Sandalio, L.M., Corpas, F.J., del Río, L.A. and Palma, J.M. (2004) Reactive Oxygen Species-Mediated Enzymatic Systems Involved in the Oxidative Action of 2,4-Dichlorophenoxyacetic Acid. Plant, Cell & Environment, 27, 1135-1148.
https://doi.org/10.1111/j.1365-3040.2004.01219.x
[10]  Mediouni, C., Benzarti, O., Tray, B., Ghorbel, M.H. and Jemal, F. (2006) Cadmium and Copper Toxicity for Tomato Seedlings. Agronomy for Sustainable Development, 26, 227-232. https://doi.org/10.1051/agro:2006008
[11]  Henriksen, A. and Selmer-Olsen, A.R. (1970) Automatic Methods for Determining Nitrate and Nitrite in Water and Soil Extracts. Analyst, 95, 514-518.
https://doi.org/10.1039/an9709500514
[12]  Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitative Determination of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-254.
https://doi.org/10.1016/0003-2697(76)90527-3
[13]  Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) Colorimetric Method for Determination of Sugars and Related Substances. Analytic Chemistry, 28, 350-356. https://doi.org/10.1021/ac60111a017
[14]  Robin, P. (1979) Etude de quelques conditions d’extraction du nitrate réductase des racines et des feuilles de plantules de ma?s. Physiologie Végétale, 17, 45-54.
[15]  Losada, M. and Paneque, A. (1971) Nitrite Reductase. Methods in Enzymology, 23, 487-491. https://doi.org/10.1016/S0076-6879(71)23120-7
[16]  Wallsgrove, R.M., Lea, P.J. and Miflin, B.J. (1979) Distribution of the Enzymes of Nitrogen Assimilation within the Pea Leaf Cell. Plant Physiology, 63, 232-236.
https://doi.org/10.1104/pp.63.2.232
[17]  Magalhaes, J.R. and Huber, D.M. (1991) Free Ammonia, Free Amino Acid and Enzyme Activity in Maize Tissue Treated with Methionine sulfoximine. Journal of Plant Nutrition, 14, 883-895. https://doi.org/10.1080/01904169109364249
[18]  Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685. https://doi.org/10.1038/227680a0
[19]  Bovet, L., Rossi, L. and Lugon-Moulin, N.C. (2006) Cadmium Partitioning and Gene Expression Studies in Nicotiana tabacum and Nicotiana rustica. Physiologia Plantarum, 128, 466-475. https://doi.org/10.1111/j.1399-3054.2006.00756.x
[20]  Wang, R., Guegler, K., LaBrie, S.T. and Crawford, N.M. (2000) Genomic Analysis of Nutrient Response in Arabidopsis Reveals Diverse Expression Patterns and Novel Metabolic and Potential Regulatory Genes Induced by Nitrate. The Plant Cell, 12, 1491-1510. https://doi.org/10.1105/tpc.12.8.1491
[21]  Huang, H. and Xiong, Z.T. (2009) Toxic Effects of Cadmium, Acetochlor and Bensulfuron-Methyl on Nitrogen Metabolism and Plant Growth in Rice Seedlings. Pesticide Biochemistry and Physiology, 94, 64-67.
https://doi.org/10.1016/j.pestbp.2009.04.003
[22]  Lugon-Moulin, N., Zhang, M., Gadani, F., Rossi, L., Koller, D., Krauss, M. and Wagner, G.J. (2004) Critical Review of the Science and Options for Reducing Cadmium in Tobacco (Nicotiana tabacum L.) and Other Plants. Advances in Agronomy, 83, 111-180. https://doi.org/10.1016/S0065-2113(04)83003-7
[23]  Takabayashi, M., Wilkerson, F.P. and Robertson, D. (2005) Response of Glutamine Synthetase Gene Transcription and Enzyme Activity to External Nitrogen Sources in the Diatom Skeletonema costatum (Baillariophyceae). Journal of Phycology, 41, 84-94. https://doi.org/10.1111/j.1529-8817.2005.04115.x
[24]  Abd-ElBaki, G.K., Siefritz, F., Man, H.M., Weiner, H., Haldenhoff, R. and Kaiser, W. (2000) Nitrate Reductase in Zea mays L. under Salinity. Plant, Cell and Environment, 23, 515-521. https://doi.org/10.1046/j.1365-3040.2000.00568.x
[25]  Lillo, C., Meyer, C., Lea, U.S., Provan, F. and Olteda, S. (2004) Mechanism and Importance of Post-Translational Regulation of Nitrate Reductase. Journal of Experimental Botany, 55, 1275-1282. https://doi.org/10.1093/jxb/erh132
[26]  Klein, D., Morcuende, R., Stitt, M. and Krapp, A. (2000) Regulation of Nitrate Reductase Expression in Leaves by Nitrate and Nitrogen Metabolism Is Completely Overridden When Sugars Fell below a Critical Level. Plant, Cell and Environment, 23, 863-871. https://doi.org/10.1046/j.1365-3040.2000.00593.x
[27]  Ezzine, M. and Ghorbel, M.H. (2006) Physiological and Biochemical Responses Resulting from Nitrite Accumulation in Tomato (Lycopersicon esculentum Mill. cv. Ibiza F1). Journal of Plant Physiology, 163, 1032-1039.
https://doi.org/10.1016/j.jplph.2005.07.013

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