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A Possible Mechanism of DNA to DNA Transcription in Eukaryotic Cells: Endonuclease Dependent Transcript Cutout

DOI: 10.4236/oalib.1102758, PP. 1-4

Subject Areas: Cell Biology, Molecular Biology, Genetics

Keywords: Plasmodium falciparum, Endonuclease Dependent Transcript Cutout, Mechanism, Eukaryotic Cell, Chromatin Conformation, Dynamic Genome Architecture, DNA to DNA Transcription, Nicking Endonuclease, ssDNA Transcript, Cell Cycle Progression, Chloroquine

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We previously proposed the existence of DNA to DNA transcription in eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA) transcript is produced and released from the genome remains unknown. We once speculated that the mechanism of DNA to DNA transcription might be similar to that of DNA to RNA transcription, but now we propose that endonuclease dependent transcript cutout may be a possible mechanism of DNA to DNA transcription, in which a copy of ssDNA fragment (transcript) between two nicks produced by nicking endonuclease is released from double-stranded DNA (dsDNA) region in the genome by an unknown ssDNA fragment releasing enzyme. The gap in the dsDNA will be filled through DNA repair mechanism. Occasionally, multiple copies of ssDNA transcripts could be produced through multiple rounds of cutout- repair-cutout cycle.

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Li, G. (2016). A Possible Mechanism of DNA to DNA Transcription in Eukaryotic Cells: Endonuclease Dependent Transcript Cutout. Open Access Library Journal, 3, e2758. doi:


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