The
present study was conducted to determine the biodegradation of petrol by Pseudomonas aeruginosa isolated from
petrol contaminated soil. The isolated strain was able to grow in minimal broth
along with 2.5%, 5%, 7.5% and 10% concentrations of petrol which indicated the
capability of the organism in degrading petrol and utilizing it as a source for
growth. The isolated strain’s efficiency was determined by analyzing the
parameters pH, optical density and CO2 released during petrol
degradation. High Performance Liquid Chromatography (HPLC) analysis also
confirmed the degradation of petrol by Pseudomonas
aeruginosa.
Cite this paper
Darsa, K. V. and Thatheyus, A. J. (2014). Biodegradation of Petroleum Compound Using Pseudomonas aeruginosa. Open Access Library Journal, 1, e735. doi: http://dx.doi.org/10.4236/oalib.1100735.
Kvenvolden, K.A. and Cooper, C.K. (2003) Natural Seepage of Crude Oil into the Marine Environment. Geo-Marine Letters, 23, 140-146. http://dx.doi.org/10.1007/s00367-003-0135-0
Mahvi, A.H. and Mardani, G. (2005) Determination of Phenanthrene in Urban Runoff of Tehran, Capital of Iran. Iranian Journal of Environmental Health, Science and Engineering, 2, 5-11.
Mohan, S.V., Shailaja, S., Ramakrishna, M., Reddy, K. and Sarma, P. (2006) Bioslurry Phase Degradation of DEP Contaminated Soil in Periodic Discontinuous Mode Operation: Influence of Augmentation and Substrate Partition. Process Biochemistry, 41, 644-652. http://dx.doi.org/10.1016/j.procbio.2005.08.018
Zhao, H., Wang, L., Ren, J., Li, Z., Li, M. and Gao, H. (2008) Isolation and Characterization of Phenanthrene-Degrading Strains Sphingomonas sp. ZP1 and Tistrella sp. ZP5. Journal of Hazardous Materials, 152, 1293-1300. http://dx.doi.org/10.1016/j.jhazmat.2007.08.008
Surridge, A.K.J. (2007) Denaturing Gradient Gel Electrophoresis Characterisation of Microbial Communities in Polycyclic Aromatic Hydrocarbon and Polychlorinated Biphenyl Contaminated Soil. PhD Thesis, University of Pretoria, Pretoria.
Ganjidoust, H. and Naghizadeh, G. (2005) Interaction’s Effect of Organic Material and Aggregation on Extraction Efficiency of Tphs from Petroleum Contaminated Soils with MAE. Iran Journal of Environmental Health Science and Engineering, 2, 213-220. http://diglib.tums.ac.ir/pub/magmng/pdf/2566.pdf
Samimi, S.V., Rad, R.A. and Ghanizadeh, F. (2009) Polycyclic Aromatic Hydrocarbon Contamination Levels in Collected Samples from Vicinity of a Highway. Iran Journal of Environmental Health Science and Engineering, 6, 47-52. http://dx.doi.org/10.1186/956727
Delnavaz, M., Ayati, B. and Ganjidoust, H. (2008) Biodegradation of Aromatic Amine Compounds Using Moving Bed Biofilm Reactors. Iran Journal of Environmental Health Science and Engineering, 5, 243-250. http://ijehse.tums.ac.ir/files/journals/1/articles/176/public/176-177-1-PB.pdf
Mohan, S.V., Prasanna, D., Reddy, B.P. and Sarma, P. (2008) Ex Situ Bioremediation of Pyrene Contaminated Soil in Bio-Slurry Phase Reactor Operated in Periodic Discontinuous Batch Mode: Influence of Bioaugmentation. International Biodeterioration and Biodegradation, 62, 162-169. http://dx.doi.org/10.1016/j.ibiod.2008.01.006
Anastasi, A., Coppola, T., Prigione, V. and Varese, G. (2009) Short Communication: Pyrene Degradation and Detoxification in Soil by a Consortium of Basidiomycetes Isolated from Compost: Role of Laccases and Peroxidases. Journal of Hazardous Materials, 165, 1229-1233. http://dx.doi.org/10.1016/j.jhazmat.2008.10.032
Larsen, S., Karakashev, D., Angelidaki, I. and Schmidt, J. (2009) Ex-Situ Bioremediation of Polycyclic Aromatic Hydrocarbons in Sewage Sludge. Journal of Hazardous Materials, 164, 1568-1572. http://dx.doi.org/10.1016/j.jhazmat.2008.08.067
Muckian, L., Grant, R., Clipson, N. and Doyle, E. (2009) Bacterial Community Dynamics during Bioremediation of Phenanthrene- and Fluoranthene-Amended Soil. International Biodeterioration & Biodegradation, 63, 52-56. http://dx.doi.org/10.1016/j.ibiod.2008.04.005
Erdogan, E.E. and Karaca, A. (2011) Bioremediation of Crude Oil Polluted Soils. Asian Journal of Biotechnology, 3, 206-213. http://dx.doi.org/10.3923/ajbkr.2011.206.213
Ladousse, A. and Tramier, B. (1991) Results of 12 Years of Research in Spilled Oil Bioremediation. International Oil Spill Conference Proceedings, American Petroleum Institute, Washington, 1991, 577-581. http://dx.doi.org/10.7901/2169-3358-1991-1-577
Leahy, J.G. and Colwell, R.R. (1990) Microbial Degradation of Hydrocarbons in the Environment. Microbiological Reviews, 54, 305-315. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC372779/
Nocentini, M., Pinelli, D. and Fava, F. (2000) Bioremediation of a Soil Contaminated by Hydrocarbon Mixtures: The Residual Concentration Problem. Chemosphere, 41, 1115-1123. http://dx.doi.org/10.1016/S0045-6535(00)00057-6
Da Cruz, F.G., De Vasconcellos, P.S., Angolini, F.F.C., Dellagnezze, M.B., Garcia, N.S.I., De Oliveira, M.V., Neto, S.D.V.E. and Marsaioli, A.J. (2011) Could Petroleum Biodegradation Be a Joint Achievement of Aerobic and Anaerobic Microorganisms in Deep Sea Reservoirs? AMB Express, 1, 47. http://dx.doi.org/10.1186/2191-0855-1-47
Oh, Y.S., Sim, D.S. and Kim, S.J. (2001) Effects of Nutrients on Crude Oil Biodegradation in the Upper Intertidal Zone. Marine Pollution Bulletin, 42, 1367-1372. http://dx.doi.org/10.1016/S0025-326X(01)00166-7
Berwick, P.G. (2004) Physical and Chemical Conditions for Microbial Oil Degradation. Biotechnology and Bioengineering, 26, 1294-1305. http://dx.doi.org/10.1002/bit.260261106
Xu, R., Yong, L.C., Lim, Y.G. and Obbard, J.P. (2005) Use of Slow-Release Fertilizer and Biopolymers for Stimulating Hydrocarbon Biodegradation in Oil-Contaminated Beach Sediments. Marine Pollution Bulletin, 51, 1101-1110. http://dx.doi.org/10.1016/j.marpolbul.2005.02.037
Toledo, F.L., Calvo, C., Rodelas, B. and Gonzdlezlopez, J. (2006) Selection and Identification of Bacteria Isolated from Waste Crude Oil with Polycyclic Aromatic Hydrocarbons Removal Capabilities. Systematic and Applied Microbiology, 29, 244-252. http://dx.doi.org/10.1016/j.syapm.2005.09.003
Song, R., Hua, Z., Li, H. and Chen, J. (2006) Biodegradation of Petroleum Hydrocarbons by Two Pseudomonas aeruginosa Strains with Different Uptake Modes. Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, 41, 733-748. http://dx.doi.org/10.1080/10934520600575135
Ueno, A., Ito, Y., Yamamoto, Y., Yumoto, I. and Okuyama, H. (2006) Bacterial Community Changes in Diesel-Oil- Contaminated Soil Microcosms Stimulation with Luria-Bertani Medium or Bioaugmintation with Petroleum-Degrading Bacterium, Pseudomonas aeruginosa Strain WatG. Journal of Basic Microbiology, 46, 310-317. http://dx.doi.org/10.1002/jobm.200510116
Das, K. and Mukherjee, A.K. (2007) Crude Petroleum-Oil Biodegradation Efficiency of Bacillus subtilis and Pseudomonas aeruginosa Strains Isolated from Petroleum-Oil Contaminated Soil from North-East India. Bioresource Technology, 98, 1339-1345. http://dx.doi.org/10.1016/j.biortech.2006.05.032
Mittal, A. and Singh, A. (2009) Isolation of Hydrocarbon Degrading Bacteria from Soil Contaminated with Crude Oil Spills. Indian Journal of Experimental Biology, 47, 760-765. http://nopr.niscair.res.in/handle/123456789/5981
Verma, S., Saxena, J., Prasanna, R., Sharma, V. and Nain, L. (2012) Medium Optimization for a Novel Crude-Oil Degrading Lipase from Pseudomonas aeruginosa SL-72 Using Statistical Approaches for Bioremediation of Crude-Oil. Biocatalysis and Agricultural Biotechnology, 1, 321-329. http://dx.doi.org/10.1016/j.bcab.2012.07.002
Texas Research Institute, Inc. (1982) Enhancing the Microbial Degradation of Underground Gasoline by Increasing Available Oxygen. Report to the American Petroleum Institute, Washington.
Englert, C.J., Kenzie, E.J. and Dragun, J. (1993) Bioremediation of Petro-leum Products in Soil. In: Calabrese, E.J. and Kostecki, P.T., Eds., Principles and Practices for Petroleum Contaminated Soils, Lewis Publishers, Chelsea, MI, 111- 130.
Waksman, S.A. (1922) A Method of Counting the Number of Fungi in Soil. Journal of Microbiology, 7, 339-341. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC378974/pdf/jbacter01062-0041.pdf
Bushnell, L.D. and Haas, H.F. (1941) The Utilization of Certain Hydrocarbons by Microorganisms. Journal of Bacteriology, 41, 653. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374727/pdf/jbacter00729-0101.pdf
Vidhya, R. and Thatheyus, A.J. (2013) Biodegradation of Dimethylformamide Using Bacillus subtilis. American Journal of Microbiological Research, 1, 10-15. http://dx.doi.org/10.12691/ajmr-1-1-3
Fritsche, W. and Hofrichter, M. (2000) Aerobic Degradation by Microorganisms. In: Klein, J., Ed, Environmental Processes-Soil Decontamination, Wiley-VCH, Weinheim, 146-155.
Sathishkumar, M., Binupriya, A.R., Baik, S.H. and Yun, S.E. (2008) Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydro-carbon Contaminated Areas. Clean, 36, 92-96.
Pepi, M., Minacci, A., Di Cello, F., Baldi, F. and Fani, R. (2003) Long-Term Analysis of Diesel Fuel Consumption in a Co-Culture of Acinetobacter venetianus, Pseudomonas putida and Alcaligenes faecalis. Antonie van Leeuwenhoek, 83, 3-9. http://www.ncbi.nlm.nih.gov/pubmed/12755474 http://dx.doi.org/10.1023/A:1022930421705
Onifade, A.K. and Abubakar, F.A. (2007) Characterization of Hydrocarbon-Degrading Microorganisms Isolated from Crude Oil Contaminated Soil and Remediation of the Soil by Enhanced Natural Attenuation. Research Journal of Biological Sciences, 2, 36-40. http://medwelljournals.com/abstract/?doi=rjbsci.2007.36.40
Hamme, J.D.V. (1999) Influences of Chemical Surfactants on the Biodegradation of Crude Oil by a Mixed Bacterial Culture. Canadian Journal of Microbiology, 45, 130-137. http://www.nrcresearchpress.com/doi/pdf/10.1139/w98-209 http://dx.doi.org/10.1139/w98-209
Leathy, J.G. and Colwell, R.R. (1990) Microbial Degradation of Hydrocarbons in the Environment. Microbiological Reviews, 54, 305-315. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC372779/
Kilbane, J., Ranganathan, R., Cleveland, L., Kayser, K.J., Ribiero, C. and Linhares, M.M. (2000) Selective Removal of Nitrogen from Quinoline and Petroleum by Pseudomonas ayucida IGTN 9M. Applied and Environmental Microbiology, 66, 688-693. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91882/pdf/am000688.pdf http://dx.doi.org/10.1128/AEM.66.2.688-693.2000
Boshui, C., Nan, Z., Jiang, W., Jiu, W. and Jianhua, F. (2012) Biodegradation of Petroleum Hydrocarbon by Pseudomonas Aeruginosa. China Petroleum Processing and Petrochemical Technology, 14, 66-70. http://www.chinarefining.com/EN/volumn/volumn_1145_abs.shtml
Genow, G., Naeyer, F., Van Meenen, P., Van de Werf, H., Nijs, W. and Verstraete, W. (1994) Degradation of Oil Sludge by Landfarming—A Case-Study at the Ghent Harbor. Biodegradation, 5, 37-46.
Wongsa, P., Tanaka, M., Ueno, A., Hasanuzzaman, M., Yumoto, I. and Okuyama, H. (2004) Isolation and Characterization of Novel Strains of Pseudomonas aeruginosa and Serratia marcescens Possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel Oil, and Lubricating Oil. Current Microbiology, 49, 415-422. http://dx.doi.org/10.1007/s00284-004-4347-y
Margesin, R. and Schinner, F. (2001) Biodegradation and Bioremediation of Hydrocarbons in Extreme Environments. Applied Microbiology and Biotechnology, 56, 650-663. http://dx.doi.org/10.1007/s002530100701