全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

Biodegradation of Petroleum Compound Using Pseudomonas aeruginosa

DOI: 10.4236/oalib.1100735, PP. 1-9

Subject Areas: Microbiology, Environmental Sciences

Keywords: Biodegradation, Hydrocarbons, Pseudomonas aeruginosa, Petrol

Full-Text   Cite this paper   Add to My Lib

Abstract

The present study was conducted to determine the biodegradation of petrol by Pseudomonas aeruginosa isolated from petrol contaminated soil. The isolated strain was able to grow in minimal broth along with 2.5%, 5%, 7.5% and 10% concentrations of petrol which indicated the capability of the organism in degrading petrol and utilizing it as a source for growth. The isolated strain’s efficiency was determined by analyzing the parameters pH, optical density and CO2 released during petrol degradation. High Performance Liquid Chromatography (HPLC) analysis also confirmed the degradation of petrol by Pseudomonas aeruginosa.

Cite this paper

Darsa, K. V. and Thatheyus, A. J. (2014). Biodegradation of Petroleum Compound Using Pseudomonas aeruginosa. Open Access Library Journal, 1, e735. doi: http://dx.doi.org/10.4236/oalib.1100735.

References

[1]  Kvenvolden, K.A. and Cooper, C.K. (2003) Natural Seepage of Crude Oil into the Marine Environment. Geo-Marine Letters, 23, 140-146.
http://dx.doi.org/10.1007/s00367-003-0135-0
[2]  Mahvi, A.H. and Mardani, G. (2005) Determination of Phenanthrene in Urban Runoff of Tehran, Capital of Iran. Iranian Journal of Environmental Health, Science and Engineering, 2, 5-11.
[3]  Mohan, S.V., Shailaja, S., Ramakrishna, M., Reddy, K. and Sarma, P. (2006) Bioslurry Phase Degradation of DEP Contaminated Soil in Periodic Discontinuous Mode Operation: Influence of Augmentation and Substrate Partition. Process Biochemistry, 41, 644-652.
http://dx.doi.org/10.1016/j.procbio.2005.08.018
[4]  Zhao, H., Wang, L., Ren, J., Li, Z., Li, M. and Gao, H. (2008) Isolation and Characterization of Phenanthrene-Degrading Strains Sphingomonas sp. ZP1 and Tistrella sp. ZP5. Journal of Hazardous Materials, 152, 1293-1300.
http://dx.doi.org/10.1016/j.jhazmat.2007.08.008
[5]  Surridge, A.K.J. (2007) Denaturing Gradient Gel Electrophoresis Characterisation of Microbial Communities in Polycyclic Aromatic Hydrocarbon and Polychlorinated Biphenyl Contaminated Soil. PhD Thesis, University of Pretoria, Pretoria.
[6]  Ganjidoust, H. and Naghizadeh, G. (2005) Interaction’s Effect of Organic Material and Aggregation on Extraction Efficiency of Tphs from Petroleum Contaminated Soils with MAE. Iran Journal of Environmental Health Science and Engineering, 2, 213-220.
http://diglib.tums.ac.ir/pub/magmng/pdf/2566.pdf
[7]  Samimi, S.V., Rad, R.A. and Ghanizadeh, F. (2009) Polycyclic Aromatic Hydrocarbon Contamination Levels in Collected Samples from Vicinity of a Highway. Iran Journal of Environmental Health Science and Engineering, 6, 47-52.
http://dx.doi.org/10.1186/956727
[8]  Delnavaz, M., Ayati, B. and Ganjidoust, H. (2008) Biodegradation of Aromatic Amine Compounds Using Moving Bed Biofilm Reactors. Iran Journal of Environmental Health Science and Engineering, 5, 243-250.
http://ijehse.tums.ac.ir/files/journals/1/articles/176/public/176-177-1-PB.pdf
[9]  Mohan, S.V., Prasanna, D., Reddy, B.P. and Sarma, P. (2008) Ex Situ Bioremediation of Pyrene Contaminated Soil in Bio-Slurry Phase Reactor Operated in Periodic Discontinuous Batch Mode: Influence of Bioaugmentation. International Biodeterioration and Biodegradation, 62, 162-169.
http://dx.doi.org/10.1016/j.ibiod.2008.01.006
[10]  Anastasi, A., Coppola, T., Prigione, V. and Varese, G. (2009) Short Communication: Pyrene Degradation and Detoxification in Soil by a Consortium of Basidiomycetes Isolated from Compost: Role of Laccases and Peroxidases. Journal of Hazardous Materials, 165, 1229-1233.
http://dx.doi.org/10.1016/j.jhazmat.2008.10.032
[11]  Larsen, S., Karakashev, D., Angelidaki, I. and Schmidt, J. (2009) Ex-Situ Bioremediation of Polycyclic Aromatic Hydrocarbons in Sewage Sludge. Journal of Hazardous Materials, 164, 1568-1572.
http://dx.doi.org/10.1016/j.jhazmat.2008.08.067
[12]  Muckian, L., Grant, R., Clipson, N. and Doyle, E. (2009) Bacterial Community Dynamics during Bioremediation of Phenanthrene- and Fluoranthene-Amended Soil. International Biodeterioration & Biodegradation, 63, 52-56.
http://dx.doi.org/10.1016/j.ibiod.2008.04.005
[13]  Erdogan, E.E. and Karaca, A. (2011) Bioremediation of Crude Oil Polluted Soils. Asian Journal of Biotechnology, 3, 206-213.
http://dx.doi.org/10.3923/ajbkr.2011.206.213
[14]  Keery, E. (1993) Bioremediation of Experimental Petroleum Spills in the Vestfold Hills, Antarctica. Polar Biology, 13, 163-170.
[15]  Ladousse, A. and Tramier, B. (1991) Results of 12 Years of Research in Spilled Oil Bioremediation. International Oil Spill Conference Proceedings, American Petroleum Institute, Washington, 1991, 577-581.
http://dx.doi.org/10.7901/2169-3358-1991-1-577
[16]  Leahy, J.G. and Colwell, R.R. (1990) Microbial Degradation of Hydrocarbons in the Environment. Microbiological Reviews, 54, 305-315.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC372779/
[17]  Nocentini, M., Pinelli, D. and Fava, F. (2000) Bioremediation of a Soil Contaminated by Hydrocarbon Mixtures: The Residual Concentration Problem. Chemosphere, 41, 1115-1123.
http://dx.doi.org/10.1016/S0045-6535(00)00057-6
[18]  Head, I.M., Jones, D.M. and Roling, W.F.M. (2006) Marine Microorganisms Make a Meal of Oil. Nature Reviews Microbiology, 4, 173-182.
http://dx.doi.org/10.1038/nrmicro1348
[19]  Da Cruz, F.G., De Vasconcellos, P.S., Angolini, F.F.C., Dellagnezze, M.B., Garcia, N.S.I., De Oliveira, M.V., Neto, S.D.V.E. and Marsaioli, A.J. (2011) Could Petroleum Biodegradation Be a Joint Achievement of Aerobic and Anaerobic Microorganisms in Deep Sea Reservoirs? AMB Express, 1, 47.
http://dx.doi.org/10.1186/2191-0855-1-47
[20]  Oliveira, P.L.F., Vasconcellos, S.P., Angolini, C.F.F., da Cruz, G.F., Marsaioli, A.J., Neto, E.V.S. and Oliviera, V.M. (2012) Taxonomic Diversity and Biodegradation Potentialof Bacteria Isolated from Oil Reservoirs of an Offshore Southern Brazilian Basin. Journal of Petroleum and Environmental Biotechnology, 3, 132.
[21]  Oh, Y.S., Sim, D.S. and Kim, S.J. (2001) Effects of Nutrients on Crude Oil Biodegradation in the Upper Intertidal Zone. Marine Pollution Bulletin, 42, 1367-1372.
http://dx.doi.org/10.1016/S0025-326X(01)00166-7
[22]  Berwick, P.G. (2004) Physical and Chemical Conditions for Microbial Oil Degradation. Biotechnology and Bioengineering, 26, 1294-1305.
http://dx.doi.org/10.1002/bit.260261106
[23]  Xu, R., Yong, L.C., Lim, Y.G. and Obbard, J.P. (2005) Use of Slow-Release Fertilizer and Biopolymers for Stimulating Hydrocarbon Biodegradation in Oil-Contaminated Beach Sediments. Marine Pollution Bulletin, 51, 1101-1110.
http://dx.doi.org/10.1016/j.marpolbul.2005.02.037
[24]  Toledo, F.L., Calvo, C., Rodelas, B. and Gonzdlezlopez, J. (2006) Selection and Identification of Bacteria Isolated from Waste Crude Oil with Polycyclic Aromatic Hydrocarbons Removal Capabilities. Systematic and Applied Microbiology, 29, 244-252.
http://dx.doi.org/10.1016/j.syapm.2005.09.003
[25]  Song, R., Hua, Z., Li, H. and Chen, J. (2006) Biodegradation of Petroleum Hydrocarbons by Two Pseudomonas aeruginosa Strains with Different Uptake Modes. Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, 41, 733-748.
http://dx.doi.org/10.1080/10934520600575135
[26]  Ueno, A., Ito, Y., Yamamoto, Y., Yumoto, I. and Okuyama, H. (2006) Bacterial Community Changes in Diesel-Oil- Contaminated Soil Microcosms Stimulation with Luria-Bertani Medium or Bioaugmintation with Petroleum-Degrading Bacterium, Pseudomonas aeruginosa Strain WatG. Journal of Basic Microbiology, 46, 310-317.
http://dx.doi.org/10.1002/jobm.200510116
[27]  Das, K. and Mukherjee, A.K. (2007) Crude Petroleum-Oil Biodegradation Efficiency of Bacillus subtilis and Pseudomonas aeruginosa Strains Isolated from Petroleum-Oil Contaminated Soil from North-East India. Bioresource Technology, 98, 1339-1345.
http://dx.doi.org/10.1016/j.biortech.2006.05.032
[28]  Mittal, A. and Singh, A. (2009) Isolation of Hydrocarbon Degrading Bacteria from Soil Contaminated with Crude Oil Spills. Indian Journal of Experimental Biology, 47, 760-765.
http://nopr.niscair.res.in/handle/123456789/5981
[29]  Verma, S., Saxena, J., Prasanna, R., Sharma, V. and Nain, L. (2012) Medium Optimization for a Novel Crude-Oil Degrading Lipase from Pseudomonas aeruginosa SL-72 Using Statistical Approaches for Bioremediation of Crude-Oil. Biocatalysis and Agricultural Biotechnology, 1, 321-329.
http://dx.doi.org/10.1016/j.bcab.2012.07.002
[30]  Zobell, C.E. (1946) Action of Microorganisms on Hydrocarbons. Bacteriological Review, 10, 1-49.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC440899/pdf/bactrev00176-0004.pdf
[31]  Texas Research Institute, Inc. (1982) Enhancing the Microbial Degradation of Underground Gasoline by Increasing Available Oxygen. Report to the American Petroleum Institute, Washington.
[32]  Englert, C.J., Kenzie, E.J. and Dragun, J. (1993) Bioremediation of Petro-leum Products in Soil. In: Calabrese, E.J. and Kostecki, P.T., Eds., Principles and Practices for Petroleum Contaminated Soils, Lewis Publishers, Chelsea, MI, 111- 130.
[33]  APHA-AWWA-WEF (1995) Standard Methods for the Examination of Water and Wastewater. 19th Edition, Washington DC.
[34]  Waksman, S.A. (1922) A Method of Counting the Number of Fungi in Soil. Journal of Microbiology, 7, 339-341.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC378974/pdf/jbacter01062-0041.pdf
[35]  Bushnell, L.D. and Haas, H.F. (1941) The Utilization of Certain Hydrocarbons by Microorganisms. Journal of Bacteriology, 41, 653.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374727/pdf/jbacter00729-0101.pdf
[36]  Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (1994) Bergey’s Manual of Determinative Bacteriology. 9th Edition, Williams & Wilkins, Baltimore.
[37]  Plummer, M.U. and Plummer, D.T. (1988) Introduction to Practical Biochemistry. Tata McGraw-Hill Education, Noida.
[38]  Cappuccino, J.G. and Sherman, N. (2001) Microbiology: A Laboratory Manual. 6th Edition, Benjamin Cummings, San Francisco.
[39]  Vidhya, R. and Thatheyus, A.J. (2013) Biodegradation of Dimethylformamide Using Bacillus subtilis. American Journal of Microbiological Research, 1, 10-15.
http://dx.doi.org/10.12691/ajmr-1-1-3
[40]  Zar, J.H. (2010) Biostatistical Analysis. Prentice Hall, Upper Saddle River, 944.
[41]  Fritsche, W. and Hofrichter, M. (2000) Aerobic Degradation by Microorganisms. In: Klein, J., Ed, Environmental Processes-Soil Decontamination, Wiley-VCH, Weinheim, 146-155.
[42]  Bello, Y.M. (2007) Biodegradation of Lagoma Crude Oil Using Pig Dung. African Journal of Biotechnology, 6, 2821- 2825.
[43]  Oliver, B. and Magot, M. (2005) Indigenous Microbial Communities in Oil Fields. In: Petroleum Microbiol, ASM Press, Washington DC, 21-34.
[44]  Rosenberg, E.R. (1996) Bioremediation of Petroleum Contamination. In: Crawford, R.L. and Crawford, D.L., Eds., Bioremediation: Principles and Applications, Cambridge University Press, Cambridge, 100-124.
http://dx.doi.org/10.1017/CBO9780511608414.006
[45]  Sathishkumar, M., Binupriya, A.R., Baik, S.H. and Yun, S.E. (2008) Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydro-carbon Contaminated Areas. Clean, 36, 92-96.
[46]  Pepi, M., Minacci, A., Di Cello, F., Baldi, F. and Fani, R. (2003) Long-Term Analysis of Diesel Fuel Consumption in a Co-Culture of Acinetobacter venetianus, Pseudomonas putida and Alcaligenes faecalis. Antonie van Leeuwenhoek, 83, 3-9.
http://www.ncbi.nlm.nih.gov/pubmed/12755474
http://dx.doi.org/10.1023/A:1022930421705
[47]  Antai, S.P. (1990) Biodegradation of Bonny Light Crude Oil by Ba-cillus sp. and Pseudomonas sp. Waste Management, 10, 61-64.
http://dx.doi.org/10.1016/0956-053X(90)90070-2
[48]  Onifade, A.K. and Abubakar, F.A. (2007) Characterization of Hydrocarbon-Degrading Microorganisms Isolated from Crude Oil Contaminated Soil and Remediation of the Soil by Enhanced Natural Attenuation. Research Journal of Biological Sciences, 2, 36-40.
http://medwelljournals.com/abstract/?doi=rjbsci.2007.36.40
[49]  Hamme, J.D.V. (1999) Influences of Chemical Surfactants on the Biodegradation of Crude Oil by a Mixed Bacterial Culture. Canadian Journal of Microbiology, 45, 130-137.
http://www.nrcresearchpress.com/doi/pdf/10.1139/w98-209
http://dx.doi.org/10.1139/w98-209
[50]  Leathy, J.G. and Colwell, R.R. (1990) Microbial Degradation of Hydrocarbons in the Environment. Microbiological Reviews, 54, 305-315.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC372779/
[51]  Kilbane, J., Ranganathan, R., Cleveland, L., Kayser, K.J., Ribiero, C. and Linhares, M.M. (2000) Selective Removal of Nitrogen from Quinoline and Petroleum by Pseudomonas ayucida IGTN 9M. Applied and Environmental Microbiology, 66, 688-693.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91882/pdf/am000688.pdf
http://dx.doi.org/10.1128/AEM.66.2.688-693.2000
[52]  Boshui, C., Nan, Z., Jiang, W., Jiu, W. and Jianhua, F. (2012) Biodegradation of Petroleum Hydrocarbon by Pseudomonas Aeruginosa. China Petroleum Processing and Petrochemical Technology, 14, 66-70.
http://www.chinarefining.com/EN/volumn/volumn_1145_abs.shtml
[53]  Genow, G., Naeyer, F., Van Meenen, P., Van de Werf, H., Nijs, W. and Verstraete, W. (1994) Degradation of Oil Sludge by Landfarming—A Case-Study at the Ghent Harbor. Biodegradation, 5, 37-46.
[54]  Atlas, R.M. (1992) Oil Spills: Regulation and Biotechnology: Guest Editorial. Current Opinion in Biotechnology, 3, 220-223.
http://dx.doi.org/10.1016/0958-1669(92)90095-Z
[55]  Wongsa, P., Tanaka, M., Ueno, A., Hasanuzzaman, M., Yumoto, I. and Okuyama, H. (2004) Isolation and Characterization of Novel Strains of Pseudomonas aeruginosa and Serratia marcescens Possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel Oil, and Lubricating Oil. Current Microbiology, 49, 415-422.
http://dx.doi.org/10.1007/s00284-004-4347-y
[56]  Margesin, R. and Schinner, F. (2001) Biodegradation and Bioremediation of Hydrocarbons in Extreme Environments. Applied Microbiology and Biotechnology, 56, 650-663.
http://dx.doi.org/10.1007/s002530100701

Full-Text


comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133

WeChat 1538708413