This research was aimed at enhancing the
production of ligninolytic enzymes produced by fungus Polyporus sp. isolated from Buleleng as a means to treat the
textile waste water. The optimum enzyme production was carried out by adding
agricultural waste such as banana skin, straw, and sawdust. The research was
completed in several stages including i) growing the fungus Polyporus sp. in PDA media, ii) enzymes
production, iii) enzyme activity tests, and iv) decomposition analysis of
textile waste water in several enzyme concentrations and in various incubation
time lengths. The results showed that the optimum activities of laccase, MnP,
and LiP enzymes that were produced without the addition of any agricultural
waste were 20.5, 25.7, and 75.4 μmol/mL·minute. On the
other hand, the enzymes activities on the addition of banana skins were 139.0,
116.0, and 654.0 μmol/mL·minute, on the addition of sawdust were 194.4, 41.0,
and 259.0, and on the addition of straw were 148.2, 131.0, and 392.0 μmol/mL·minute,
respectively. The efficiency of the degradation of 25 mL of remazol black B and
remazol red RB (100 mg/L) by using 6 mL ligninolytic enzyme during the six-hour
incubation was 53% - 58% and 55.4% - 69.3%, respectively.
Cite this paper
Sukarta, I. N. and Sastrawidana, I. D. K. (2014). The Use of Agricultural Waste to Increase the Production Ligninolytic Enzyme by Fungus Polyporus sp.. Open Access Library Journal, 1, e642. doi: http://dx.doi.org/10.4236/oalib.1100642.
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