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Search Results: 1 - 10 of 93930 matches for " 吴美娇 "
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柱前衍生化-高效液相色谱法跟踪检测酶法制备γ-D-谷氨酰-L-色氨酸反应过程
屠春燕,吴燕娇,袁艳娟,姚忠,唐美华,韦萍
分析化学 , 2009,
Abstract: γ-D-谷氨酰-L-色氨酸(SCV-07)是一种新型广谱免疫调节类化合物.针对酶法合成SCV-07反应体系的特点,以邻硝基苯磺酰氯为柱前衍生试剂,RP-HPLC为分离模式,选用LichrospherC18柱,20mmol/L磷酸盐溶液(pH3.0)和乙腈为流动相,梯度洗脱,检测波长230nm,建立了同时分析测定多种氨基酸和小肽的方法,所有组分于15min内洗脱完毕.氨基酸及二肽在0.96～260μmol/L范围内线性良好,相关系数大于0.9991;加标回收率在97.8%～101.6%之间;相对标准偏差为1.2%～2.9%.本方法适用于SCV-07产品的纯度分析,以及酶法转肽制备SCV-07反应过程跟踪检测,具有分析速度快,准确度高、操作简单等优点.
95份百合种质资源花粉量的测定与散粉特性分析
王蓝青,吴美娇,王雪倩,房伟民,陈发棣,滕年军
南京农业大学学报 , 2018, DOI: 10.7685/jnau.201801045
Abstract: [目的]本文旨在研究影响百合散粉的相关因素，为培育无花粉污染百合品种奠定基础。[方法]以95份百合种质资源为试材，调查其花粉量和与散粉特性相关的花器性状。[结果]95份百合种质资源中，3份没有花药，其余92份均含有花药，花粉量变幅为0~192.5万粒，其中喇叭百合平均花粉量最高，分别是亚洲百合、铁亚百合杂种系（LA百合）、铁东百合杂种系（LO百合）、东喇百合杂种系（OT百合）、东方百合和野百合的4.34、3.35、3.32、1.52、1.60和1.59倍。花药体积变幅为0~609 mm3，其中喇叭百合花药平均体积最大为494 mm3，分别是上述其他6个系列的4.66、3.41、2.51、1.21、1.57和1.64倍。野百合花药中花粉密度最大，平均为644粒？mm-3，分别为亚洲百合、LA百合、LO百合、OT百合、东方百合和喇叭百合的1.27、1.10、1.52、1.38、1.12和1.14倍。4份百合种质资源未散粉，其中3份没有花药而不散粉。‘不锈钢’因花药不开裂而不散粉；‘耶罗琳’等花粉中含有大量油滴，花粉彼此黏着力较大，散粉时形成较大的花粉团，尽管花粉量较多，但花粉污染较轻；而‘德利安娜’等花粉含有油滴较少，花粉彼此黏着力较小，散粉时不易形团状，花粉污染较重。[结论]大部分百合种质资源花粉量较多，花粉污染严重；百合花粉量主要由花药大小决定，受花药中花粉密度影响较小；表观花粉量或花粉污染程度与花粉量成正相关，而与花粉粒之间黏着力度成负相关。
[Objectives] The article aims to study the relevant factors that affect the lily pollen dispersal, which aims to lay the foundation for cultivating pollen-contaminated lily varieties. [Methods] 95 lily germplasm resources were used as experimental materials to analyze the pollen quantity and the floral organ traits related to loose powder characteristics. [Results] There was no anther in 3 lily germplasm resources, and in other 92 germplasm resources all had anthers. The content of single flower powder ranged from 0 to 192.5×104. Among them, trumpet lily had the highest average pollen content, which was 4.34, 3.35, 3.32, 1.52, 1.60 and 1.59 times higher than that of the Asiatic lily, Longiflorum/Asiatic hybrid (LA)lily, Longiflorum/oriental hybrid (LO)lily, oriental/trumpet hybrid (OT)lily, oriental lily and wild lily, respectively. The variation of anther volume in 95 lily germplasm resources ranged from 0 to 609 mm3, and the largest average volume of anthers was 494 mm3 in trumpet lily, which were 4.66, 3.41, 2.51, 1.21, 1.57 and 1.64 times of other 6 series mentioned above, respectively. The largest pollen density was in wild lily anther, with an average of 644 seeds per mm3, which were 1.27, 1.10, 1.52, 1.38, 1.12 and 1.14 times in Asiatic lily, LA lily, LO lily, OT lily, oriental lily and trumpet lily, respectively. The 4 lily germplasm resources did not disperse, among which 3 germplasm resources had no anthers. The anther of ‘Stainless Steel’ was indehiscence;the pollen of ‘Yelloween’ contained a large amount of oil droplets, with high pollen adhesion to each other which was easy to form regiments. It was not easy to cause pollution despite that it owned numerous pollens;however, the pollen of ‘Deliana’ contained a small quantity of oil droplets and low pollen cohesion which was hard to form regiments and caused pollution. [Conclusions] Most lily germplasm resources contained various pollens and cause serious
李维汉统战思想及其当代价值研究
Research of the Modern Theoretical Value of Li Weihan United Front Tactics  [PDF]
吴美, 任芳
Advances in Social Sciences (ASS) , 2012, DOI: 10.12677/ASS.2012.13018
Abstract: 李维汉同志是德高望重的老一辈无产阶级革命家。在半个多世纪生涯中，他为中国共产党的建设，为中国人民革命的胜利和社会主义事业的发展，特别是为中国人民统一战线的发展壮大，做出了重大的突出的贡献。他以马克思列宁主义、毛泽东思想为指导，从事统一战线的理论政策研究，提出一系列重要理论观点和政策主张，丰富了马克思列宁主义、毛泽东思想理论宝库。他的理论贡献，不仅在当时对统一战线工作起了重要的指导作用，而且对新时期的统一战线工作仍然具有重要的指导意义和许多的有益启示。
Comrade Li Weihan is respected an older generation of proletarian revolutionaries. During his half a century career, he made outstanding contribution for the Communist Party of China, for the victory of the Chinese People’s Revolution and the development of the cause of socialism, especially for the development and growth of the Chinese People’s United Front. With the guidance of Marxism-Leninism, Mao Zedong Thoughts, he devoted himself to the theory of the united front policy research, and also put forward a series of important theoretical policy which then enriched Marxism-Leninism and Mao Zedong Thoughts. His theoretical contribution not only played an important role in guiding work of the united front at that historical background, but also worked as a useful tool for the united front work in the new period.
美国清前期研究的新趋势
（美）柯娇燕,高翔
清史研究 , 1988,
Abstract: ？
求解随机广义互补问题的期望残差最小化方法
Expected Residual Minimization Method for Stochastic Generalized Complementary Problems  [PDF]
罗美菊, 吴欧
Advances in Applied Mathematics (AAM) , 2012, DOI: 10.12677/AAM.2012.11002
Abstract: 由于广义互补问题有着广泛的应用，并且在实际应用中存在很多不确定因素。因此，本文主要考虑随机广义互补问题。通过所谓的NCP函数给出它的期望残差最小化(ERM)模型。由于所给出的ERM模型中含有一个积分计算。一般情况下，积分计算很难得到精确值。因此，本文引入拟蒙特卡罗方法，并用此方法给出ERM问题的近似问题。进一步，证明了在一定条件下，由ERM问题的近似问题得到的解的序列收敛到ERM问题的解。
 In practice, generalized complementary problems have many applications and many elements may involve uncertain data in applications. Therefore, we mainly consider the stochastic generalized complementary problems. We employ the so called NCP function to give the expected residual minimization (ERM) model. Since the ERM formulation includes an integration, which is generally difficult to evaluate exactly, we propose the quasi-Monte Carlo method to give an approximation problem for ERM formulation. Furthermore, we show that the solutions of this approximation problem converge to the solution of the ERM formulation under very mild conditions.
一位国小教师资优数学课程的创意教学设计与实施
Creative Teaching Design and Implementation of an Elementary Teacher on Gifted Math Program  [PDF]
徐伟民, 吴伶娇, 刘祥通
Advances in Education (AE) , 2014, DOI: 10.12677/AE.2014.43B002
Abstract:

本研究旨在探讨一位国小专家教师如何设计与实施资优数学课程与教学。以教学观察、访谈和文件等方式来进行一学期的数据搜集，结果发现个案教师设计多样化、生活性与高认知的数学问题，透过主题式探索与讨论的教学实施，以培养学生的数学思考、推理、沟通、连结与表征等能力，并引导学生进行探索性的独立研究。个案教师资优数学课程的设计与实施，是根据她个人的教学理念、先备经验和兴趣，在没有特定教学进度的压力和家长的支持下，她依据资优课程的目标、学生的需求和喜好，来进行数学课程的设计与实施，也因此引发学生探索数学的兴趣，并将学生探索的结果参加数学科展竞赛，获得良好的成绩表现。

The purpose of this study was to investigate how an expert elementary teacher designed and implemented gifted mathematics curriculum and teaching. Teaching observation, interviews and document were used to collect data for one semester, and the results indicated that mathematics problems the teacher designed were diverse, life-related, and classified as high cognitive problems. Exploration and discussion were used to solve the problems in order to foster students’ abilities on mathematical thinking, reasoning, communication, connection and representation, and guide students to conduct independent study for getting more exploration. Mathematics curriculum design and implementation of the teacher was based on her personal teaching ideas, prior experiences and interests, and in the context of parents’ support and without pressure from specific teaching progression, she integrated the objectives of gifted curriculum, students’ needs and interests into the design and implementation of the mathematics curriculum. Thus, her teaching evoked students’ interest to explore mathematics further. The teacher also used the results of students’ exploration to attend mathematics competition in elementary level, and got a good grade and performance.



陆地棉、澳洲棉和绿顶棉三元杂种的细胞遗传学研究
Cytogenetic Study on Trispecific Hybrid from Gossypium hirsutum L., G. australe and G. capitis-viridis  [PDF]
李灵娇, 陈弟, 吴玉香
Hans Journal of Agricultural Sciences (HJAS) , 2013, DOI: 10.12677/HJAS.2013.34018
Abstract: 棉属有丰富的种质资源，野生棉具有栽培种所缺乏的许多优良性状，为了将野生澳洲棉和绿顶棉的优良性状导入陆地棉，以期改良栽培种陆地棉。本研究通过陆地棉和C组野生澳洲棉远缘杂交获得杂种F1，并对其进行染色体加倍，将加倍成的异源六倍体与B组野生绿顶棉杂交，产生陆地棉、澳洲棉、绿顶棉的三元杂种，并对该三元杂种花粉母细胞减数分裂行为进行观察。结果表明：三元杂种减数分裂异常，主要表现在：后期染色体不均等分离，使得末期形成大量的不正常多分孢子，其中以五分体居多，这些多分孢子进一步发育为大量败育花粉粒，导致该杂种高度不育。本研究不仅为该三元杂种不育提供了直接原因，也为进一步探讨有效的育性恢复方法及棉花新种质的创制提供了理论依据和中间材料。
 There are rich germplasm resources in Gossypium and wild cotton species possess many valuable agronomic traits which cultivated cotton always lacks. In order to introgress the useful traits from wild species G. australe and G. capitis-viridis into cultivated species for improving upland cotton, tetraploid G. hirsutum was crossed directly with C-genome species G. australe as the pollen-providing parent, creating a triploid hybrid. Chromosome doubling of this triploid hybrid leads to an allohexaploid which was then crossed with B-genome wild species G. capitis-viridis, resulting in trispecific hybrid (G. hirsutum, G. australe and G. capitis-viridis). The characteristic of chromosome behavior during meiosis of trispecific hybrid F1 was further investigated in this study. Results indicated that the meiosis of trispecific hybrid was abnormal, which mainly reflected that the chromosomes were divided unequally during the anaphase II; and many abnormal multispores appeared in the telophase II, which finally developed into abortive pollen grains, directly leading to the sterility of trispecific hybrid F1. This study provides the main reason of sterility for this hybrid and supplies the theory basis and the effective method for restoring the fertility and further creating new cotton intermediate germplasms.
中美过去300年土地利用变化比较
何凡能,李美娇,肖冉
地理学报 , 2015, DOI: 10.11821/dlxb201502010
Abstract: 开展历史土地利用变化的国别对比研究,不仅有利于深化大尺度LUCC过程与驱动机制的科学认识,而且对深入研究历史碳排放责任等也有着重要意义。利用长时间序列数据,对中美两国过去300年土地利用/覆被变化的时空特征及驱动因素进行对比分析。结果表明①过去300年中美两国耕地数量总体均呈持续增长态势,中国在1661-1980s的320余年间耕地总量增长了79.46×104km2,但人均耕地面积却从1685年的5.5亩,下降至2000年的1.5亩;而美国在1700-1950年的250年间耕地面积增加了190.87×104km2,且人均耕地面积均超过9亩,其中1890年高达30.2亩。②受土地垦殖扩张影响,过去300年中美两国林、草地资源均呈持续减少态势;其中,美国森林面积减少了约145×104km2,草地面积减少约137×104km2;而中国森林面积减少了约90×104km2,草地面积减少了约40×104km2。③从空间格局变化特征看,过去300年中国土地垦殖主要向边区（如东北、西南、内蒙等）及内地山地丘陵区发展;而美国在“西进运动”的影响下,大规模向中西部拓殖。④政策、人口、农业技术等因素是影响过去300年中美土地利用/覆被变化的主要因素,但由于两国资源禀赋及发展历史的差异,虽然直接驱动力均体现为政策因素,但就根本驱动因素而言,中国是人口数量,美国则是经济利益;同时,在农业技术发展取向上,中国因“人多地少”,走“精耕细作”之路,而美国因“人少地多”,走“机械化”之途。
环境气象学与特种气象预报
吴兑,邓雪娇
气象 , 2000, DOI: 10.7519/j.issn.1000-0526.2000.8.001
Abstract: 在人类的生活环境中，许多现象都与气象具有密切的关系，所有研究与人类生活息息相关的大气现象及其变化规律的学科，称为环境气象学。人们迫切需要了解同自己日常生活有密切关系的环境及影响环境条件的各种因素的变化状况，以便采取各种对策和措施来保护环境和保护人类自己，再加上政府部门与社会生产活动需求的增加，使特种气象预报服务应运而生。
热带沿海近地层大气的湍流结构和谱特征
邓雪娇？,吴兑？
热带气象学报 , 1997,
Abstract: 利用1990年在海南省海口市天尾村的一次观测的资料,分析了速度分量谱、湍流强度等湍流特征量。这批资料多是有云影响情况下,稳定度参数(l)与热通量(wt)没有日变化规律,但湍能、平均温度仍具有日变化规律；湍谱的基本特征与晴天天气的谱基本相似,在惯性副区接近局地各向同性,速度谱符合相似理论的“-2/3”次方规律；根据m-o相似理论,探讨了各分量湍强δi/u*(i=u,v,w)与稳定度z/l的关系,表明各分量湍强与稳定度z/l仍具有“1/3”次方规律,但湍强和湍流能量比一般平坦地形下垫面的大。
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