a sequential batch reactor (4 l) inoculated with aspergillus niger was operated in order to remove congo red dye (10 mg l-1). the feeding of the reactor was done to each 7 days. the glucose was added in the concentration of 1 g.l-1 (stage i) and 0.5 g l-1 (stage ii). the stage iii occurred without glucose addition. the stage i was great to process, because the system reached the greater dye removal (95%) as well as the kinetic parameters ware the best - km (0.7 g l-1) and k1 (0.025 h-1).