a experiment was carried out in the poultry science station of cft/ufpb with the objective to evalluate the annatto seeds bran (asb) as an ingredient and as an additive colouring of several tissue in 125 hens lohmann lsl with twenty-two weeks of age, 1.65 kg of live weight, allocated in the completely randomized design, with five treatments and five replication of five hens. the treatment 1 (t1) = diet based on corn and soybean meal; t2 = diet + 40% of sorghum; t3 = t2 + 4% asb; t4 = t2 + 8% asb; e t5 = t2 + 12% asb. the birds were fed 110 g of diet/day and water ad libitum. the collect were obtained in three periods of twenty-eight days. collor changes were measured with yolk the roche？ collor fan method (m1) and score calculated from "b+" (yellowness) of minolta？ cr-10 instrument (m2). excepting the t1 and t2 of analysis of variance, the increase of asb in diet with 40% of sorghum improved, linearly, intake, egg production, egg mass, egg mass and egg dozen feed conversion ratio. the yolk color by m1 and m2, beak by m2 and ovary by m1 improved with the increasing of asb in diets. a high correlation coefficient (r = 0.72; p < 0.001) was observed for yolk collor among m1 and m2. suggested up to 12% of asb in diet where sorghum is principal source of energy to improve yolk pigmentation and egg production.