Comparación de protocolos de extracción de ADN con muestras de aleta y larva de peces: extracción modificada con cloruro de sodio

the use of appropriate sampling methods, the type of tissue or sample and the use of viable dna extraction protocols are critical issues in studies based on pcr. in an attempt to identify a simple, reproducible, inexpensive and non-toxic method for obtaining high quality and quantity genomic dna from fish fin and larvae samples, modified salt extraction protocol and modified phenol-chloroform extraction protocol were compared. the samples obtained from different fish species (brycon orbignyanus, piaractus mesopotamicus, oreochromis niloticus, leporinas elongatus and prochilodus lineatus) using salt extraction showed good dna quality and quantity (dna/rna relationship 1.8-2.2). these dna samples were successfully used in the amplification of rapd (random amplified polymorphic dna) and microsatellite molecular markers, demonstrating the same effectiveness of this protocol in comparison with modified phenol-chloroform extraction protocol. this dna extraction procedure constitutes an alternative and efficient replacement for previous protocols for improving fish molecular studies