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Compara??o dos caldos selenito cistina, tetrationato Muller-Kauffmann e Rappaport-Vassiliadis no isolamento de Salmonella Typhimurium

DOI: 10.1590/S0102-09352012000300035

Keywords: calves, feces, selective enrichment broth, salmonella typhimurium, bezerro, fezes, meio de enriquecimento seletivo, salmonella typhimurium.

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Abstract:

three selective enrichment broths - selenite cystine (sc), muller-kauffmann tetrathionate (mkt) and rappaport-vassiliadis (rv) - were compared, for salmonella typhimurium isolation from rectal swabs of a calf experimentally infected. the bacteriological procedure involved pre-enrichment in hajna-gn broth (only for the samples inoculated in rv broth), selective enrichment (sc, mkt and rv broths), culture in modified brilliant green agar (bga), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-o and poli-h salmonella antisera. sc and mkt broths were more efficient in the isolation of salmonella typhimurium (12 positive samples), whereas rv broth had a lower efficiency in the microbiological isolation (ten positive samples).

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