双组分快速固化生物软组织黏合剂的研制
Development of double-component rapid curing bioadhesive

贻贝足丝蛋白（MFp）是一类能够在水下迅速固化，并能黏附在不同基材表面的特殊蛋白质，作为蛋白类生物黏合剂具有广泛的应用前景。本文在大肠杆菌中高效可溶性地表达了贻贝足丝融合蛋白 Sumo-Fp3（SFp3），并通过蘑菇酪氨酸酶在柱催化，将其中约 5% 的酪氨酸残基转化为 DOPA。在含 DOPA 的 SFp3（DSFp3）中加入分子量为 1 500 kD 的透明质酸后，形成的双组分生物胶水在牛皮表面的黏附力超过氰基丙烯酸盐组织粘合剂Dermabond？的两倍，并在 5 min 内达到最大黏附强度的 52% 。通过生物膜层干涉技术和扫描电子显微镜，观测到透明质酸与 DSFp3 存在静电层层组装行为，并形成紧密片层结构。本研究为蛋白质类生物胶水黏附强度低、固化慢提供了一种解决方法和理论基础。
Mussel foot proteins (MFp) could cure rapidly under water and adhere to different substrates. It has broad application prospects as an biocompatible bioglue. The soluble recombinant SUMO-MFp fusion protein (SFp3) was efficiently expressed inE.coli, and about 5% of tyrosine of SFp3 were converted into DOPA by using mushroom tyrosinase. The adhesion strength of the mixture of DOPA-containing SFp3 (DSFp3) and hyaluronic acid (MW = 1 500 kD) was more than twice that of the cyanoacrylate-based tissue adhesives, Dermabond？, and it reached 52% of its maximal strength within 5 minutes on cowhide. A layer-by-layer assembly of hyaluronic acid with DSFp3 was observed to form compact sheet structures through biofilm interferometry assay and scanning electron microscopy. This work provides a solution and theoretical basis for the low adhesion strength and slow curing of protein-based bioglue.