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Keywords Abstract

-  2018 


DOI: 10.3969/j.issn.1673-1689.2018.01.017

Keywords: 肉牛屠宰工序 菌相 PCR-DGGE 初始 污染源
slaughter process
,bacteria phase,PCR-DGGE,initial,pollution sources

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In order to extend the chilled beef shelf life and reduce initial spoilage bacteria of Calvin Wan Company,investigate the main pollution process and initial microbial source of beef becomes necessary. The distribution microbial in the air of cattle slaughterhouse and in the process of operation,such as the hands,tools and water,were analyzed by colony count and Polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) technology. The results showed the initial microorganisms carried by beef is Acinetobacter johnsonii,Staphylococcus sp.,Macrococcus caseolyticus etc. The main contamination processing were head hoof and segmentation,the main source of environmental contamination in the pre production of beef is from abattoir worker's hands,gloves,knives,chopping blocks,Tweezers,hooks,conveyor belts


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