All Title Author
Keywords Abstract

Quantitative Screening of Secretory Protein Genes in Candidatus Liberibacter Asiaticus

DOI: 10.4236/ajps.2018.912174, PP. 2408-2419

Keywords: Candidatus Liberibacter Asiaticus, Secretory Protein, DNA Amount, RNA Transcription, Subcellular Localization

Full-Text   Cite this paper   Add to My Lib


Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The disease is caused by Candidatus Liberibacter spp., which is vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Secretory proteins are important in bacterial pathogenesis and structure components. Some of them are expressed at a high level. To obtain the highly-expressed secretory protein genes (SPGs) for antiserum preparation, six candidate SPGs were chosen from Candidatus


[1]  Bové, J.M. (2006) Huanglongbing: A Destructive, Newly-Emerging, Century-Old Disease of Citrus. Journal of Plant Pathology, 88, 7-37.
[2]  Gottwald, T.R. (2010) Current Epidemiological Understanding of Citrus Huanglongbing. Annual Review of Phytopathology, 48, 119-139.
[3]  Arratia-Castro, A.A., Santos-Cervantes, M.E., Arce-Leal, á.P., Espinoza-Mancillas, M.G., Rodríguez Negrete, E.A., Méndez-Lozano, J., Arocha-Rosete, Y. and Leyva-López, N.E. (2016) Detection and Quantification of ‘Candidatus Phytoplasma asteris' and ‘Candidatus Liberibacter Asiaticus' at Early and Late Stages of Huanglongbing Disease Development. Canadian Journal of Plant Pathology, 38, 411-421.
[4]  Fan, G.C., Bo, L., Ru-Jian, W.U., Tao, L.I., Cai, Z.J. and Chong, K.E. (2009) Thirty Years of Research on Citrus Huanglongbing in China. Fujian Journal of Agricultural Sciences, 24,183-190.
[5]  Bai, Z.Q. (2012) Occurrence Dynamics of Citrus Huanglongbing in P.R. China and Population Differentitation of ‘Candidatus Liberibacter Asiaticus’. Master Dissertation, Southwest University, Chongqing.
[6]  Da, G.J., Douhan, G.W., Halbert, S.E., Keremane, M.L., Lee, R.F., Vidalakis, G. and Zhao, H. (2016) Huanglongbing: An Overview of a Complex Pathosystem Ravaging the World’s Citrus. Journal of Integrative Plant Biology, 58, 373-387.
[7]  Villechanoux, S., Garnier, M., Renaudin, J. and Bové, M. (1992) Detection of Several Strains of the Bacterium-Like Organism of Citrus Greening Disease by DNA Probes. Current Microbiology, 24, 89-95.
[8]  Gottwald, T.R., Graça, J.V.D. and Bassanezi, R.B. (2007) Citrus Huanglongbing: The Pathogen and Its Impact. Plant Health Progress, 1, 0906-0901.
[9]  Iftikhar, Y., Rauf, S., Shahzad, U. and Zahid, M.A. (2016) Huanglongbing: Pathogen Detection System for Integrated Disease Management—A Review. Journal of the Saudi Society of Agricultural Sciences, 15, 1-11.
[10]  Munir, S., He, P., Wu, Y., He, P., Khan, S., Huang, M., Cui, W., He, P. and He, Y. (2018) Huanglongbing Control: Perhaps the End of the Beginning. Microbial Ecology, 76, 192-204.
[11]  Li, W., Laurene, L. and Johns, H. (2009) Quantitative Distribution of ‘Candidatus Liberibacter Asiaticus’ in Citrus Plants with Citrus Huanglongbin. Phytopathology, 99, 139-144.
[12]  Lu, L., Fan, G., Hu, X., Zhang, L., Huang, Z. and Chen, G. (2011) PCR Detection of Huanglongbing Pathogen in Different Parts of Citrus Plants in the Field and Analysis of the Cause of the Disease. Plant Protection, 23, 271-312.
[13]  Wu, X., Meng, C., Wang, G., Liu, Y., Zhang, X., Yi, K. and Peng, J. (2016) Rapid and Quantitative Detection of Citrus Huanglongbing Bacterium ‘Candidatus Liberibacter Asiaticus’ by Real-Time Fluorescent Loop-Mediated Isothermal Amplification Assay in China. Physiological & Molecular Plant Pathology, 94, 1-7.
[14]  Vetten, H.J., Ehlers, U. and Paul, H.L. (2010) Detection of Potato Viruses Y and A in Tubers by Enzyme-Linked Immunosorbent Assay after Natural and Artificial Break of Dormancy. Journal of Phytopathology, 108, 41-53.
[15]  Selvarajan, R., Balasubramanian, V. and Gayathrie, T. (2016) Highly Efficient Immunodiagnosis of Episomal Banana Streak MY Virus Using Polyclonal Antibodies Raised against Recombinant Viral-Associated Protein. Journal of Phytopathology, 164, 497-508.
[16]  Webster, C.G., Turechek, W.W., Li, W., Kousik, C.S. and Adkins, S. (2016) Development and Evaluation of ELISA and qRT-PCR for Identification of Squash Vein Yellowing Virus in Cucurbits. Plant Disease, 101, 178-185.
[17]  Costa, T.R., Felisberto-Rodrigues, C., Meir, A., Prevost, M.S., Redzej, A., Trokter, M. and Waksman, G. (2015) Secretion Systems in Gram-Negative Bacteria: Structural and Mechanistic Insights. Nature Reviews Microbiology, 13, 343-359.
[18]  Yu, L.Z., Liu, F.J., Yan, N.N., Cheng, X., Li, Y.Z. and Guo, Y.Z. (2017) Progress on Secretion Systems and Secretory Products of Gram-Negative Bacteria. Progress in Veterinary Medicine, 38, 80-84.
[19]  Clark, K., Franco, J.Y., Schwizer, S., Pang, Z., Hawara, E., Liebrand, T.W.H., Pagliaccia, D., Zeng, L., Gurung, F.B., Wang, P., Shi, J., Wang, Y., Ancona, V., van der Hoorn, R.A.L., Wang, N., Coaker, G. and Ma, W. (2018) An Effector from the Huanglongbing-Associated Pathogen Targets Citrus Proteases. Nature Communications, 9, 1718.
[20]  Hao, J. (2011) Construction Expression and Purification Research of Citrus Huanglongbing Serralysin Protein. Master Dissertation, Huazhong Agricultural University, Wuhan.
[21]  Hou, W.Z. (2014) Expression and Purification of Citrus Huanglongbing Serralysin and Molecular Detection of Two Important Pathogens. Master Dissertation, Huazhong Agricultural University, Wuhan.
[22]  Duan, Y.P., Zhou, L.J., Hall, D.G., Li, W.B., Doddapaneni, H., Lin, H., Liu, L., Vahling, C.M., Gabriel, D.W. and Williams, K.P. (2009) Complete Genome Sequence of Citrus Huanglongbing Bacterium, “Candidatus Liberibacter Asiaticus” Obtained through Metagenomics. Molecular Plant-Microbe Interactions, 22, 1011-1020.
[23]  Yan, J., Yuan, F., Long, G., Qin, L. and Deng, Z. (2012) Selection of Reference Genes for Quantitative Real-Time RT-PCR Analysis in Citrus. Molecular Biology Reports, 39, 1831-1838.
[24]  Sparkes, I.A. and Al, E. (2006) Rapid, Transient Expression of Fluorescent Fusion Proteins in Tobacco Plants and Generation of Stably Transformed Plants. Nature Protocols, 1, 2019-2025.
[25]  Barny, M.A., Gaudriault, S., Brisset, M.N. and Paulin, J.P. (1999) HRP Secreted Proteins: Their Role in Pathogenicity and HR Elicitation. Acta Horticulturae, 489, 353-358.
[26]  Abby, S.S. and Rocha, E.P.C. (2016) Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder. Methods in Molecular Biology, 1615, 1-21.
[27]  Li, T., Xu, Z., Zhang, T., Li, L., Chen, H. and Zhou, R. (2012) The Genetic Analysis of the Flp Locus of Actinobacillus pleuropneumoniae. Archives of Microbiology, 194, 167-176.
[28]  Zav’Yalov, V., Zavialov, A., Zav’Yalova, G. and Korpela, T. (2010) Adhesive Organelles of Gram-Negative Pathogens Assembled with the Classical Chaperone/Usher Machinery: Structure and Function from a Clinical Standpoint. FEMS Microbiology Reviews, 34, 317-378.
[29]  Okuda, M., Matsumoto, M., Tanaka, Y., Subandiyah, S. and Iwanami, T. (2005) Characterization of the tufB-secE-nusG-rplKAJL-rpoB Gene Cluster of the Citrus Greening Organism and Detection by Loop-Mediated Isothermal Amplification. Plant Disease, 89, 705-711.
[30]  Li, W., Hartung, J.S. and Levy, L. (2006) Quantitative Real-Time PCR for Detection and Identification of Candidatus Liberibacter Species Associated with Citrus Huanglongbing. Journal of Microbiological Methods, 66, 104-115.
[31]  Tanaka, F.A.O., Coletta-Filho, H.D., Alves, K.C.S., Spinelli, M.O., Machado, M.A. and Kitajima, E.W. (2007) Detection of the “Candidatus Liberibacter Americanus” in Phloem Vessels of Experimentally Infected Cataranthus Roseus by Scanning Electron Microscopy Detecção de Candidatus Liberibacter americanus em vasos de floema de Catharantus roseus infectados exper. Tropical Plant Pathology, 32, 519-519.
[32]  Fujikawa, T., Miyata, S. and Iwanami, T. (2013) Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium “Candidatus Liberibacter Asiaticus” by Direct PCR from the Midrib Extract. PLoS ONE, 8, e57011.
[33]  Fang, D., Paul, C., Brlansky, R. and Hartung, J.S. (2017) Immune Tissue Print and Immune Capture-PCR for Diagnosis and Detection of Candidatus Liberibacter Asiaticus. Scientific Reports, 7, Article No. 46467.
[34]  Yuan, Q., Jordan, R., Brlansky, R.H., Minenkova, O. and Hartung, J. (2016) Development of Single Chain Variable Fragment (scFv) Antibodies against Surface Proteins of “Ca. Liberibacter Asiaticus”. Journal of Microbiological Methods, 122, 1-7.
[35]  Liu, H., Atta, S. and Hartung, J.S. (2017) Characterization and Purification of Proteins Suitable for the Production of Antibodies against “Ca. Liberibacter Asiaticus”. Protein Expression and Purification, 139, 36-42.
[36]  Pagliaccia, D., Shi, J., Pang, Z., Hawara, E., Clark, K., Thapa, S.P., De Francesco, A.D., Liu, J., Tran, T.T., Bodaghi, S., Folimonova, S.Y., Ancona, V., Mulchandani, A., Coaker, G., Wang, N., Vidalakis, G. and Ma, W. (2017) A Pathogen Secreted Protein as a Detection Marker for Citrus Huanglongbing. Frontiers in Microbiology, 8, 2041.


comments powered by Disqus