All Title Author
Keywords Abstract

Two Methods for Calculating the Size Distribution of Ferritin’s Outer Diameter

DOI: 10.4236/cmb.2018.83006, PP. 115-121

Keywords: Ferritin, Outer Diameter, Density Distribution, Calculation Method

Full-Text   Cite this paper   Add to My Lib


Ferritin is a kind of iron-storage protein widely found in animals and plants. The dynamic light scattering (Dynamic Light Scattering) method is used in the laboratory to determine the ferritin size. This paper presents two methods for calculating the outer diameter size distribution of ferritin, both of which assume that ferritin is approximately spherical. The ferritin data file was obtained from the PDB website and was calculated using the coordinate data of the amino acids to which the amino acids belong. The first method is based on the calculation of the sphere center; the second method is based on the method of the farthest distance atom pair. The outer diameter size distribution curves obtained by the two methods are basically consistent with the experimental methods. The paper also compares the calculation results and performance of the two methods. Both methods are versatile and can be used to calculate the size distribution of the globular proteins.


[1]  Liu, B.J. and Zhang, W.B. (2009) Structure, Function and Expression Manipulation of the Iron Protein. Feed Industry, 4, 42-47.
[2]  Knovich, M.A., et al. (2009) Ferritin for the Clinician. Blood Reviews, 23, 95-104.
[3]  Lee, L.A. and Wang, Q. (2006) Adaptations of Nanoscale Viruses and Other Protein Cages for Medical Applications. Nanomedicine, 2, 137-149.
[4]  Kwon, K.C., et al. (2014) Proteinticle/Gold Core/Shell Nanoparticles for Targeted Cancer Therapy without Nanotoxicity. Advanced Materials, 26, 6436-6441.
[5]  Lee, B.-R., et al. (2016) Engineered Human Ferritin Nanoparticles for Direct Delivery of Tumor Antigens to Lymph Node and Cancer Immunotherapy. Scientific Reports, 6, 35182.


comments powered by Disqus

Contact Us


微信:OALib Journal