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Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes

DOI: 10.4236/ajps.2017.88123, PP. 1801-1819

Keywords: Cork oak, Quercus suber L., Nodes, Internodes, Callogenesis

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Abstract:

The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting; small white embryos appear on the surface of calluses.

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