The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn
from seedlings of three months. These latter were obtained after acorns
germination on peat. Nodes showed a high capacity for callogenesis and the best
rate was obtained on a medium containing Woody Plant Medium (WPM)
macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of
6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of
Sucrose. Calluses are transplanted onto the same mineral solution, with or
without growth regulators, and in the 3rd transplanting; small white embryos
appear on the surface of calluses.
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