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Expression Vector Construction and Genetic Transformation of Paeonia lactiflora Gibberellin 20-Oxidase Gene

DOI: 10.4236/ajps.2017.87105, PP. 1525-1533

Keywords: PlGA20ox, Expression Vector, Arabidopsis thaliana, Transformation

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Abstract:

GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA20ox for future gene engineering in peony.

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