Vitamin A palmitate (VAP) contains retinol and palmitic acid which is easily absorbed by body and widely used in skin care products. But, it is a hydrophobic and oxidation sensitive molecule which undergoes rapid degradation especially in an aqueous environment. The purpose of this study was to prepare microcapsules of VAP using combination maltodextrin and modified starches. Emulsion of VAP was prepared using cremophore RH 40 with Tween 80 in a homogenizer and formed emulsion was spray-dried. The spray process was optimized using a central composite design for two variables to obtain microcapsules with desirable characteristics. Microcapsules containing 30% of VAP were produced using different concentration of wall materials. The prepared microcapsules were evaluated for their physical, morphological, in-vitro drug release and SEM study. The results showed that obtained microcapsules are nearly spherical in shape with a particle size ranged from 1 to 12 μm. The drug content and encapsulation efficiency (53% - 63%) of different batches were found within acceptable range. These stabilized drug loaded microcapsules were incorporated into silicone cream based formulation for convenient topical application and evaluated for its physicochemical parameters. The drug release study showed 80.18% to 83.43% of drug release from VAP microcapsules while topical formulations prepared by VAP microcapsules showed 67.09% to 71.45% drug release at the end of 24 hrs. The formulations were kept for 3 months stability study as per ICH guidelines and found to be stable.
West, C.E., Eilander, A. and van Lieshout, M. (2002) Consequences of Revised Estimates of Carotenoid Bioefficacy for Dietary Control of Vitamin A Deficiency in Developing Countries. The Journal of Nutrition, 132, 2920S-2926S.
Sauvant, P., Cansell, M., Sassi, A.H. and Atgié, C. (2012) Vitamin A Enrichment: Caution with Encapsulation Strategies Used for Food Applications. Food Research International, 46, 469-479. https://doi.org/10.1016/j.foodres.2011.09.025
Promislow, J.H., Goodman-Gruen, D., Slymen, D.J. and Barrett-Connor, E. (2002) Retinol Intake and Bone Mineral Density in the Elderly: The Rancho Bernardo Study. Journal of Bone and Mineral Research, 17, 1349-1358.
Semenova, E.M., Cooper, A., Wilson, C.G. and Converse, C.A. (2002) Stabilization of All-Trans-Retinol by Cyclodextrins: A Comparative Study Using HPLC and Fluorescence Spectroscopy. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 44, 155-158. https://doi.org/10.1023/A:1023042612880
Lesmes, U. and McClements, D.J. (2009) Structure-Function Relationships to Guide Rational Design and Fabrication of Particulate Food Delivery Systems. Trends in Food Science & Technology, 20, 448-457. https://doi.org/10.1016/j.tifs.2009.05.006
Fuchs, M., Turchiuli, C., Bohin, M., Cuvelier, M., Ordonnaud, C., Peyrat-Maillard, M., et al. (2006) Encapsulation of Oil in Powder Using Spray Drying and Fluidised Bed Agglomeration. Journal of Food Engineering, 75, 27-35.
Chiu, Y., Chiu, C., Chien, J., Ho, G., Yang, J. and Chen, B. (2007) Encapsulation of Lycopene Extract from Tomato Pulp Waste with Gelatin and Poly(γ-Glutamic Acid) as Carrier. Journal of Agricultural and Food Chemistry, 55, 5123-5130.
Ersus, S. and Yurdagel, U. (2007) Microencapsulation of Anthocyanin Pigments of Black Carrot (Daucus carota L.) by Spray Drier. Journal of Food Engineering, 80, 805-812. https://doi.org/10.1016/j.jfoodeng.2006.07.009
Tuyen, C.K., Nguyen, M.H. and Roach, P.D. (2010) Effects of Spray Drying Conditions on the Physicochemical and Antioxidant Properties of the Gac (Momordica cochinchinensis) Fruit Aril Powder. Journal of Food Engineering, 98, 385-392.
Drusch, S., Serfert, Y., Van Den Heuvel, A. and Schwarz, K. (2006) Physicochemical Characterization and Oxidative Stability of Fish Oil Encapsulated in an Amorphous Matrix Containing Trehalose. Food Research International, 39, 807-815.
Kolanowski, W., Laufenberg, G. and Kunz, B. (2004) Fish Oil Stabilisation by Microencapsulation with Modified Cellulose. International Journal of Food Sciences and Nutrition, 55, 333-343. https://doi.org/10.1080/09637480410001725157
Balasubramani, P., Palaniswamy, P., Visvanathan, R., Thirupathi, V., Subbarayan, A. and Maran, J.P. (2015) Microencapsulation of Garlic Oleoresin Using Maltodextrin as Wall Material by Spray Drying Technology. International Journal of Biological Macromolecules, 72, 210-217. https://doi.org/10.1016/j.ijbiomac.2014.08.011
Junyaprasert, V.B., Mitrevej, A., Sinchaipanid, N., Boonme, P. and Wurster, D.E. (2001) Effect of Process Variables on the Microencapsulation of Vitamin A Palmitate by Gelatin-Acacia Coacervation. Drug Development and Industrial Pharmacy, 27, 561-566. https://doi.org/10.1081/DDC-100105181
Fule, R., Meer, T., Amin, P., Dhamecha, D. and Ghadlinge, S. (2014) Preparation and Characterisation of Lornoxicam Solid Dispersion Systems Using Hot Melt Extrusion Technique. Journal of Pharmaceutical Investigation, 44, 41-59.
Wang, Y., Lu, Z., Lv, F. and Bie, X. (2009) Study on Microencapsulation of Curcumin Pigments by Spray Drying. European Food Research and Technology, 229, 391-396. https://doi.org/10.1007/s00217-009-1064-6
Patil, A.K., Gangurde, A.B. and Amin, P.D. (2014) Comparative Study of Different Formulations of Aceclofenac as a Topical Drug Delivery System and It’s in Vitro and in Vivo Characterization. International Journal of Pharmaceutical Sciences and Research, 5, 3401.
Kamble, S., Udapurkar, P., Nakhat, P., Yeole, P. and Biyani, K. (2011) Development and Evaluation of Sorbitan Monostearate Organogels as a Topical Delivery System for Aceclofenac. Indian Journal of Pharmaceutical Education and Research, 45, 65-70.
Segall, A., Sosa, M., Alami, A., Enero, C., Hormaechea, F., Pizzorno, M., et al. (2004) Stability Study of Lipoic Acid in the Presence of Vitamins A and E in o/w Emulsions for Cosmetic Application. Journal of Cosmetic Science, 55, 449-462.
Nenadis, N. and Tsimidou, M. (2002) Observations on the Estimation of Scavenging Activity of Phenolic Compounds Using Rapid 1,1-Diphenyl-2-picrylhydrazyl (DPPH•) Tests. Journal of the American Oil Chemists’ Society, 79, 1191-1195.
Erasto, P., Bojase-Moleta, G. and Majinda, R.R. (2004) Antimicrobial and Antioxidant Flavonoids from the Root Wood of Bolusanthus speciosus. Phytochemistry, 65, 875-880. https://doi.org/10.1016/j.phytochem.2004.02.011
Pouton, C.W. (2000) Lipid Formulations for Oral Administration of Drugs: Non-Emulsifying, Self-Emulsifying and “Self-Microemulsifying” Drug Delivery Systems. European Journal of Pharmaceutical Sciences, 11, S93-S98.
Craig, D., Barker, S., Banning, D. and Booth, S. (1995) An Investigation into the Mechanisms of Self-Emulsification Using Particle Size Analysis and Low Frequency Dielectric Spectroscopy. International Journal of Pharmaceutics, 114, 103-110.
Yang, Y. and McClements, D.J. (2013) Encapsulation of Vitamin E in Edible Emulsions Fabricated Using a Natural Surfactant. Food Hydrocolloids, 30, 712-720.
Patil, V., Chauhan, A.K. and Singh, R.P. (2014) Optimization of the Spray-Drying Process for Developing Guava Powder Using Response Surface Methodology. Powder Technology, 253, 230-236. https://doi.org/10.1016/j.powtec.2013.11.033
Jaya, S. and Das, H. (2004) Effect of Maltodextrin, Glycerol Monostearate and Tricalcium Phosphate on Vacuum Dried Mango Powder Properties. Journal of Food Engineering, 63, 125-134. https://doi.org/10.1016/S0260-8774(03)00135-3
Loh, S.K., Man, C., Yaakob, B., Tan, C.P., Osman, A. and Hamid, N.S.A. (2005) Process Optimisation of Encapsulated Pandan (Pandanus amaryllifolius) Powder Using Spray-Drying Method. Journal of the Science of Food and Agriculture, 85, 1999-2004. https://doi.org/10.1002/jsfa.2169
Silva, P.I., Stringheta, P.C., Teófilo, R.F. and de Oliveira, I.R.N. (2013) Parameter Optimization for Spray-Drying Microencapsulation of Jaboticaba (Myrciaria jaboticaba) Peel Extracts Using Simultaneous Analysis of Responses. Journal of Food Engineering, 117, 538-544. https://doi.org/10.1016/j.jfoodeng.2012.08.039
Tonon, R.V. (2009) Secagem por atomização do suco de açaí: Influência das variáveis de processo, qualidade e estabilidade do produto. Universidade Estadual de Campinas, Campinas, Doutorado em Engenharia de Alimentos.