珊瑚的组织和细胞培养研究进展
Advances in the Tissue and Cell Culture of Corals

DOI: 10.12677/AMS.2016.32007, PP. 43-47

Keywords: 珊瑚细胞，细胞培养，共生作用
Coral, Cell Culture, Symbiosis

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Abstract:

珊瑚的组织和细胞的体外培养可为研究珊瑚的互惠共生、细胞毒理、环境胁迫和生物矿化等方面提供有效的研究工具和手段。本文综述了珊瑚细胞的分离与培养、珊瑚细胞团和组织球的制备及其应用，并对珊瑚的组织和细胞培养的研究前景进行了展望。
In vitro cultured tissues and cells of corals can provide a useful tool for studies on the symbiosis between coral and zooxanthellae, cytotoxicology, environmental stress and biomineralization in corals. This paper has reviewed the progress in the dissociation and culture of coral cells, prepa-ration of cell aggregates and tissue balls of corals and their application. The future prospect of the tissue and cell culture of corals is also predicted.

References

[1]	赵美霞, 余克服, 张乔民. 珊瑚礁区的生物多样性及其生态功能[J]. 生态学报, 2006, 26(1): 186-194.
[2]	Bellwood, D.R., Hughes, T.P., Folke, C., et al. (2004) Confronting the Coral Reef Crisis. Nature, 429, 827-833. http://dx.doi.org/10.1038/nature02691
[3]	Lesser, M.P. (2006) Oxidative Stress in Marine Environments: Bio-chemistry and Physiological Ecology. Annual Review of Physiology, 68, 253-278. http://dx.doi.org/10.1146/annurev.physiol.68.040104.110001
[4]	Rinkevich, B. (2011) Cell Cultures from Marine Invertebrates: New Insights for Capturing Endless Stemness. Marine Biotechnology, 13, 345-354. http://dx.doi.org/10.1007/s10126-010-9354-3
[5]	Rannou, M. (1971) Cell Culture of Invertebrates Other than Mollusksand Arthropods. In: Vago, C., Ed., Invertebrate Tissue Culture, Academic Press, New York, 385-410. http://dx.doi.org/10.1016/B978-0-12-709901-9.50019-3
[6]	Frank, U.R.C. and Rinkevich, B. (1994) In Vitro Establishment of Continuous Cell Cultures and Cell Lines from Ten Colonial Cnidarians. Marine Biology, 120, 491-499. http://dx.doi.org/10.1007/BF00680224
[7]	Helman, Y., Natale, F., Sherrell, R.M., et al. (2008) Extracellular Matrix Production and Calcium Carbonate Precipitation by Coral Cells in Vitro. Proceedings of the National Academy of Sciences, 105, 54-58. http://dx.doi.org/10.1073/pnas.0710604105
[8]	Khalesi, M.K. (2008) Cell Cultures from the Symbiotic Soft Coral Sinularia flexibilis. In Vitro Cellular & Developmental Biology-Animal, 44, 330-338. http://dx.doi.org/10.1007/s11626-008-9128-7
[9]	Reyes-Bermudez, A. and Miller, D.J. (2009) In Vitro Culture of Cells Derived from Larvae of the Staghorn Coral Acropora millepora. Coral Reefs, 28, 859-864. http://dx.doi.org/10.1007/s00338-009-0527-3
[10]	Domart-Coulon, I.J., Elbert, D.C., Scully, E.P., et al. (2001) Aragonite Crystallization in Primary Cell Cultures of Multicellular Isolates from a Hard Coral, Pocillopora damicornis. Proceedings of the National Academy of Sciences of the USA, 98, 11885-11890. http://dx.doi.org/10.1073/pnas.211439698
[11]	Huete-Stauffer, C., Valisano, L., Gaino, E., et al. (2015) Devel-opment of Long-Term Primary Cell Aggregates from Mediterranean Octocorals. In Vitro Cellular & Developmental Biology-Animal, 51, 815-826. http://dx.doi.org/10.1007/s11626-015-9896-9
[12]	Gates, R.D. and Muscatine, L. (1992) Three Methods for Iso-lating Viable Anthozoan Endoderm Cells with Their Intracellular Symbiotic Dinoflagellates. Coral Reefs, 11, 143-145. http://dx.doi.org/10.1007/BF00255468
[13]	Kopecky, E.J. and Ostrander, G.K. (1999) Isolation and Primary Culture of Viable Multicellular Endothelial Isolates from Hard Corals. In Vitro Cellular & Developmental Biology-Animal, 35, 616-624. http://dx.doi.org/10.1007/s11626-999-0101-x
[14]	Vizel, M., Loya, Y., Downs, C.A., et al. (2011) A Novel Method for Coral Explant Culture and Micropropagation. Marine Biotechnology, 13, 423-432. http://dx.doi.org/10.1007/s10126-010-9313-z
[15]	Gardner, S.G., Nielsen, D.A., Petrou, K., et al. (2015) Charac-terisation of Coral Explants: A Model Organism for Cnidarian-Dinoflagellate Studies. Coral Reefs, 34, 133-142. http://dx.doi.org/10.1007/s00338-014-1240-4
[16]	Sammarco, P.W. (1982) Polyp Bail-Out: An Escape Response to Environmental Stress and a New Means of Reproduction in Corals. Marine Ecology-Progress Series, 10, 57-65. http://dx.doi.org/10.3354/meps010057
[17]	Kramarsky-Winter, E. and Loya, Y. (1996) Regeneration versus Budding in Fungiid Corals: A Trade-Off. Marine Ecology-Progress Series, 134, 179-185. http://dx.doi.org/10.3354/meps134179
[18]	Mass, T., Drake, J.L., Haramaty, L., et al. (2012) Aragonite Precipi-tation by “Proto-Polyps” in Coral Cell Cultures. PLoS ONE, 7, e35049. http://dx.doi.org/10.1371/journal.pone.0035049
[19]	Nesa, B. and Hidaka, M. (2009) High Zooxanthella Density Shortens the Survival Time of Coral Cell Aggregates under Thermal Stress. Journal of Experimental Marine Biology and Ecology, 368, 81-87. http://dx.doi.org/10.1016/j.jembe.2008.10.018
[20]	Downs, C.A., Fauth, J.E., Downs, V.D. and Ostrander, G.K. (2010) In Vitro Cell-Toxicity Screening as an Alternative Animal Model for Coral Toxicology: Effects of Heat Stress, Sulfide, Rotenone, Cyanide, and Cuprous Oxide on Cell Viability and Mitochondrial Function. Ecotoxicology, 19, 171-184. http://dx.doi.org/10.1007/s10646-009-0403-5
[21]	Shinzato, C., Shoguchi, E., Kawashima, T., et al. (2011) Using the Acropora digitifera Genome to Understand Coral Responses to Environmental Change. Nature, 476, 320-323. http://dx.doi.org/10.1038/nature10249
[22]	Lin, S., Cheng, S., Song, B., et al. (2015) The Symbiodinium kawagutii Genome Illuminates Dinoflagellate Gene Expression and Coral Symbiosis. Science, 350, 691-694. http://dx.doi.org/10.1126/science.aad0408

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