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氮源对杜鹃花菌根真菌氮吸收及硝酸还原酶活性的影响

DOI: 10.3969/j.issn.1000-2006.2014.01.031, PP. 175-178

Keywords: 杜鹃花类菌根,氮源,氮吸收,硝酸还原酶

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Abstract:

采用菌丝干重法、半微量凯氏定氮法和磺胺比色法分别测定了hd19、zt13和d13个树粉孢属杜鹃花菌根真菌菌株在铵态氮、硝态氮和有机氮培养基中的生物量、含氮量和硝酸还原酶活性。结果表明:3个菌株在3种氮源培养基中生长良好,而且无机氮培养基中菌株生物量和氮吸收量均显著高于有机氮培养基;其中菌株hd19在硝态氮培养基中氮吸收量显著高于铵态氮,而菌株zt13和d1在铵态氮培养基中氮吸收量较高;菌株在硝态氮培养基中硝酸还原酶活性均较高,说明树粉孢属杜鹃花菌根真菌不仅具有较好的利用硝态氮的能力,还可以吸收利用其他形态的氮源,外源硝态氮可以诱导该类真菌硝酸还原酶活性的提高。

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