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一种sds-page与maldi-tof质谱联用的方法

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Abstract:

以尿激酶原为材料，探索一种从sds-page胶上回收蛋白质做maldi-tof质谱的方法.所用的回收方法包括电洗脱、脱盐、除sds等过程.电洗脱用的是高盐阻断法，脱盐用的是超滤技术，去除sds用的是冷丙酮沉淀法.结果证明，此方法至少对一些蛋白质（如尿激酶原和牛血清白蛋白）是可行的.

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