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海洋科学  2010 

Se(IV)对藻蓝蛋白分子的氧化及纳米Se(0)的生成

, PP. 55-58

Keywords: ,钝顶螺旋藻,藻蓝蛋白,光谱,纳米

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Abstract:

从钝顶螺旋藻(Spirulinaplatensis)中提取纯化藻蓝蛋白(Phycocyanin,PC),光吸收值A620/A280为3.1,观察Se(Ⅳ)与PC相互作用的光谱行为及红硒化现象。光谱检测发现,加入SeO32-后,PC在620nm的特征光吸收减弱,并随Se(Ⅳ)浓度增加和时间延长单调降低,278nm和347nm的光吸收增强;PC的荧光发射和荧光激发也均呈现衰减。另外,PC与Na2SeO3相互作用后,观察到红硒化现象,透射电镜(TEM)证明主要存在形态为红色纳米硒。结果提示PC可能是SeO32?作用的敏感靶点,Se(Ⅳ)对PC主要表现为氧化损伤,而Se(Ⅳ)被还原为红色纳米硒。

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