全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

初级基质对降解菌Pseudomonas putida共代谢苯扎贝特的影响

Keywords: 苯扎贝特,共代谢,葡萄糖,关键酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

通过对3,5-二硝基水杨酸(DNS)法测定还原糖含量各影响因素的筛选,优化确定了显色时间8min,稳定时间10min,最佳波长490nm下进行吸光度测定的检测方法;分析分别以葡萄糖和麦芽糖作为外加碳源时,降解菌PseudomonasputidaB-31的生长情况和共代谢降解典型药物苯扎贝特(BZF)的过程。结果表明,降解菌只有在外加碳源的条件下才可正常生长,而且其在葡萄糖环境中生长得更好;拟合得到的葡萄糖、麦芽糖和BZF代谢动力学结果显示,葡萄糖对BZF去除的促进作用更为明显,同时从葡萄糖培养基中降解菌所提取的酶比活力要高于麦芽糖培养基,分析原因可能是葡萄糖所诱导的降解菌关键酶活力更强,而且还可能会产生不同的蛋白质点位。

References

[1]  Andreozzi R., Raffaele M., Nicklas P., Pharmaceuticals in STP effluents and their solar photodegradation in aquatic environment.Chemosphere, 2003, 50(10):1319-1330
[2]  Rosal R., Rodea-Palomares I., Boltes K., et al.Ecotoxicity assessment of lipid regulators in water and biologically treated wastewater using three aquatic organisms.Environmental Science and Pollution Research, 2010, 17(1):135-144
[3]  Valeska C., Claudia L., Stephan P., et al.Exposure to human pharmaceuticals Carbamazepine, Ibuprofen and Bezafibrate causes molecular effects in Dreissena polymorpha.Aquatic Toxicology, 2011, 105(3-4):428-437
[4]  李开军, 张建强.共代谢在废水处理中的应用.四川环境, 2011, 30(1):111-115 Li K.J., Zhang J.Q.The application of co-metabolism in wastewater treatment.Sichuan Environment, 2011, 30(1):111-115(in Chinese)
[5]  张锡辉, Bajpai R.以关键酶为基础共代谢模型的建立——以甲烷细菌共代谢三氯乙烯为例.环境科学学报, 2000, 20(5):558-562 Zhang X.H, Bajpai R.Development of a key-enzyme based model for cometabolism-a case study on cometabolism of trichloroethylene by methanotrohpic bacteria.Acta Scientiae Circumstantiae, 2000, 20(5):558-562(in Chinese)
[6]  吴守江, 刘姝, 张晓晖.共代谢机制处理苯胺废水的研究.辽宁化工, 2012, 41(7):675-677 Wu S.J., Liu S., Zhang X.H..Research on aniline wastewater treatment by co-metabolism.Liaoning Chemical Industry, 2012, 41(7):675-677(in Chinese)
[7]  杨兴, 薛罡, 赵晓祥, 等.苯扎贝特降解菌的筛选及降解特性.微生物学报, 2010, 50(6):797-802 Yang X., Xue G., Zhao X.X., et al.Isolation of a bacterial strain capable of bezafibrate-degrading and biodegradation characteristics.Acta Microbiologica Sinica, 2010, 50(6):797-802(in Chinese)
[8]  Abshagen U., Bablok W., Koch K., et al.Disposition pharmacokinetics of bezafibrate in man.European Journal of Clinical Pharmacology, 1979, 16(1):31-38
[9]  邵锦挺, 应国清, 王琦, 等.微型化DNS法测定多糖水解液中还原糖的质量浓度.浙江工业大学学报, 2012, 40(3):250-252 Shao J.T., Ying G.Q., Wang Q., et al.Miniaturization for determination of reducing sugar mass concentration using 3, 5-dinitrosalicylic acid method.Journal of Zhejiang University of Technology, 2012, 40(3):250-252(in Chinese)
[10]  蔡丽朋, 惠秋沙.DNS法测定玉米须保健饮料中总多糖含量.齐鲁药事, 2011, 30(2):88-90 Cai L.P., Hui Q.S.Quantitative determination of polysaccharides in traditional Chinese medicine health care beverage of stigma maydis by DNS method.Qilu Pharmaceutical Affairs, 2011, 30(2):88-90(in Chinese)
[11]  胡锡阶, 肖建辉, 肖瑜, 等.3, 5-二硝基水杨酸比色法定糖的条件优化.遵义医学院学报, 2005, 28(1):9-11 Hu X.J., Xiao J.H., Xiao Y., et al.Studies on the optimal conditions for measurement of reduced sugar in a 3, 5-dinitrosalieylie acid colorimetry.Acta Academiae Medicinae Zunyi, 2005, 28(1):9-11(in Chinese)
[12]  齐香君, 苟金霞, 韩戌珺, 等.3, 5-二硝基水杨酸比色法测定溶液中还原糖的研究.纤维素科学与技术, 2004, 12(3):17-19 Qi X.J., Gou J.X., Han X.J., et al.Study on measuring reducing sugar by DNS reagent.Journal of Cellulose Science and Technology, 2004, 12(3):17-19(in Chinese)
[13]  王俊丽, 聂国兴, 李素贞, 等.DNS法测定还原糖含量时最适波长的确定.河南农业科学, 2010, 4(4):115-118 Wang J.L., Nie G.X., Li S.Z., et al.Optimal wavelength for determining the content of reducing sugar by DNS method.Journal of Henan Agricultural Sciences, 2010, 4(4):115-118(in Chinese)
[14]  余志坚, 陈传红, 赵晋宇.DNS法检测食用菌多糖含量条件优化研究.江苏农业科学, 2012, 40(1):259-260 Yu Z.J., Chen C.H., Zhao J.Y.Optimal conditions for measurement of polysaccharose in domestic fungus by DNS method.Journal of Henan Agricultural Sciences, 2012, 40(1):259-260(in Chinese)
[15]  孙伟伟, 曹维强, 王静.DNS 法测定玉米秸秆中总糖.食品研究与开发, 2006, 27(6):120-123 Sun W.W., Cao W.Q., Wang J.Colorimetric determination of water-soluble total sugar in corn stalk with 3, 5-dinitrosalicylic acid.Food Research and Development, 2006, 27(6):120-123(in Chinese)
[16]  张锡辉, 白志俳.难降解有机污染物共降解机理解析.上海环境科学, 2000, 19(7):297-301 Zhang X.H., Bai P.R.Analysis of cometabolic biodegradation of refractory organic pollutants.Shanghai Environmental Sciences, 2000, 19(7):297-301(in Chinese)
[17]  孙雪景, 王静, 焦岩, 等.微生物共代谢作用的研究与应用.农业与技术, 2010, 30(4):57-60 Sun X.J., Wang J., Jiao Y, et al.Research and application of co-metabolism in microorganism.Agriculture and Technology, 2010, 30(4):57-60(in Chinese)
[18]  Leadbetter E.R., Foster J.W.Oxidation products formed from gaseous alkane by the bacterium Pseudomonas metharica.Arch.Biochem.Biophys., 1959, 82(8):491-492
[19]  胡勇, 冯智星.共代谢及其在废水处理中的研究.装备制造, 2009, 8(1):166-167 Hu Y., Feng Z.X.Research on co-metabolism and its application in wastewater treatment.China Equipment, 2009, 8(1):166-167(in Chinese)

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133