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OALib Journal期刊
ISSN: 2333-9721
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利用人工氧还酶体系催化L-苹果酸氧化脱羧反应
DOI: 10.3724/SP.J.1088.2012.10938, PP. 530-535
Keywords: 生物催化,粗酶液,苹果酸酶,丙酮酸,人工氧还酶体系
Abstract:
？利用含人工氧还酶体系的粗酶液代替纯酶催化反应,以省去酶分离纯化过程.由苹果酸酶突变体ME-t(MEL310R/Q401C)和非天然辅酶烟酰胺5-氟胞嘧啶二核苷酸(NFCD+)组成的人工氧还酶体系可以催化氧化L-苹果酸生成丙酮酸,并得到非天然辅酶的还原态(NFCDH).利用含人工氧还酶体系的粗酶液催化反应,只得到单一产物丙酮酸,其选择性与纯酶催化的相同.来自粪肠球菌Enterococcusfaecalis的NADH氧化酶(NOX)可再生NFCD+.与含NAD+,ME粗酶液和NOX粗酶液的偶联反应体系相比,含NFCD+,ME-t粗酶液和NOX粗酶液的体系获得的丙酮酸产率高9%,而副产物乳酸明显减少.可见人工氧还酶体系使用更方便,且产物选择性更高,有望代替纯酶催化反应.这为降低生物催化剂的成本,扩大生物催化反应的应用提供了一种新的策略.
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