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Enol form of uracil in the GU "wobble" pair. NMR evidence

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Abstract:

GU "wobble" pair may contain either the enol form of uracil and the keto form of guanine or the enol form of guanine and the keto form of uracil. A direct proof of uracil existence in enol form can be obtained by using NMR spectroscopy. In HNN-COSY experiments the GU pair imitates the GC pair due to the enolization of uracil.

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