All Title Author
Keywords Abstract

Prevalence and Antibiotic Resistance Pattern of Escherichia coli and Klebsiella pneumoniae in Urine Tract Infections at the La Paz Medical Center, Malabo, Equatorial Guinea

DOI: 10.4236/ojmm.2015.54022, PP. 177-183

Keywords: Urine Tract Infections, Antibiotic Resistance, Klebsiella pneumonia, Escherichia coli

Full-Text   Cite this paper   Add to My Lib


The study was conducted to isolate and determine the antibiotic resistance in Escherichia coli and Klebsiella pneumonia from urine samples over a 2-year period (August 2013-September 2015) at the La Paz Medical Center, Malabo. A retrospective analysis of 785 urine culture samples over a 2-year period August 2013-September 2015 was carried out according to the routine protocol of urinalysis. Bacterial etiological agents were isolated from 155 (19.7%) samples with highest prevalence of Escherichia coli (55.5%) followed by Klebsiella pneumonia (23.2%), Proteus mirabilis (4.5%), Pseudomonas species (3.2%), Enterobacter species (2.6%), Enterococcus faecalis (2.6%) and others species (8.4%). The E. coli and K. pneumonia represent 78.7% of all isolated bacterial strains. The E. coli and K. pneumoniae isolates possess highly resistant to ampicillin, Trimethoprim/Sulfamethoxazole, Doxycycline, Amoxicicline/Clavulanic acid. Whereas K. pneumonia demonstrated also to be highly resistant to Gentamycin, Cefuroxime and Ceftriaxon, low level of resistance to Piperacilin/Tazobactam, Amikacin and the lowest to Imipenem. The alarming level of MDR strains to the first choice antibiotics treatment was observed.


[1]  Tansarli, G., Athanasiou, S. and Falagas, M. (2013) Evaluation of Antimicrobial Susceptibility of Enterobacteriaceae Causing Urinary Tract Infections in Africa. Antimicrobial Agents and Chemotherapy, 57, 3628-3639.
[2]  WHO (2014) Antimicrobial Resistance: Global Report on Surveillance.
[3]  Schito, G., Naber, K., Botto, H., et al. (2009) The ARESC Study: An International Survey on the Antimicrobial Resistance of Pathogens Involved in Uncomplicated Urinary Tract Infections. International Journal of Antimicrobial Agents, 34, 407-413.
[4]  Foxman, B. (2010) The Epidemiology of Urinary Tract Infection. Nature Reviews Urology, 7, 653-660.
[5]  Habte, T., Dube, S., Ismail, N. and Hoosen, A. (2009) Hospital and Community Isolates of Uropathogens at a Tertiary Hospital in South Africa. South African Medical Journal, 99, 584-587.
[6]  El Bouamri, M., Arsalane, L., El Kamouni, Y. and Zouhair, S. (2015) Antimicrobial Susceptibility of Urinary K. pneumonia and the Emergence of Carbapenem-Resistance Strain. African Journal of Urology, 21, 36-40.
[7]  Abejew, A., Denboba, A. and Mekonnen, A. (2014) Prevalence and Antibiotic Resistance Pattern of Urinary Tract Bacterial Infections in Dessie Area, North-East Ethiopia. BMC Reseach Notes, 7, 687.
[8]  Hima-Lerible, H., Menard, D. and Talarmin, A. (2003) Antimicrobial Resistance among Uropathogenes That Cause Community-Acquired Urinary Tract Infection in Bangui, Central African Republic. Journal of Antimicrobial Chemotherapy, 51, 192-194.
[9]  Randrianirina, F., Soares, J., Carod, J., et al. (2007) Antimicrobial Resistance among Uropathgens That Cause Community-Acquired Urinary Tract Infections in Antananario, Madagascar. Journal of Antimicrobial Chemotherapy, 59, 309.
[10]  Moroh, J., Fleury, Y., Tia, H., et al. (2015) Diversity and Antibiotic Resistance of Urapathogenic Bacteria from Abijan. African Journal of Urology, 20, 18-24.
[11]  Iregbu, K. and Princewill, P. (2013) Urinary Tract Infection in a Tertiary Hospital in Abuja, Nigeria. African Journal of Clinical and Experimental Microbiology, 14, 167-173.
[12]  Rerambiah, L., Ndong, J., Massoua, P., et al. (2014) Antimicrobial Profiles of Bacterial Clinical Isolates from the Gabonese National Laboratory of Public Health: Data from Routine Activity. International Journal of Infectious Diseases, 29, 48-53.
[13]  Mbaga, J., Dube, S. and Munyanduki, H. (2010) Prevalence and Drug Resistance in Bacteria of the Urinary Tract Infections in Bulawayo Province, Zimbabwe. East African Journal of Public Health, 7, 229-232.
[14]  Akoachere, J., Yvone, S., Akum, N. and Seraphine, E. (2012) Etiologic Profile and Antimicrobial Susceptibility of Community-Acquired Urinary Tract Infection in Two Cameroonian Towns. BMC Research Notes, 5, 219.
[15]  Ngwai, Y., Akpotu, M., Okidake, R., et al. (2011) Antimicrobial Susceptibility of Escherichia coli and Other Coliforms Isolated from Urine of Asymptomatic Students in Bayelsa State, Nigeria. African Journal of Microbiology Research, 5, 184-191.
[16]  Clinical Laboratory Standards Institute (2012) Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition. M02-A11, Volume 32.
[17]  Clinical Laboratory Standards Institute (2013) Performance Standards for Antimicrobial Susceptibility Testing. M100-S23, Volume 33.
[18]  Clinical Laboratory Standards Institute (2014) Performance Standards for Antimicrobial Susceptibility Testing. M100-S24, Volume 34.
[20]  Magiorakos, A., Srinivasan, A., Carey, R., et al. (2012) Multidrug-Resistant, Extensively Drug-Resistant and Pandrug-Resistant Bacteria: An International Expert Proposal for Interim Standard Definitions for Acquired Resistance. Clinical Microbiology and Infection, 18, 268-281.
[21]  Ibrahim, M., Bilal, N. and Hamid, M. (2012) Increased Multi Drug Resistant Eschrichia coli from Hospitals in Khartoum State, Sudan. African Health Sciences, 12, 368-375.
[22]  Obirikorang, C., Quaye, L., Bio, F., et al. (2012) Asyptomatic Bacteriuria among Pregnan Women Attending Antenatal Clinic at the University Hospital, Kumasi, Ghana. Journal of Medicine and Biomedical Sciences, 1, 38-44.
[23]  Eshetie, S., Unakalet, C., Gelaw, A., et al. (2015) Multidrug Resistant and Carbapenemase Producing Enterobacteriaceae among Patients with Urinary Tract Infection at Referral Hospital, Northwest Ethiopia. Antimicrobial Resistance and Infection Control, 4, 12.


comments powered by Disqus

Contact Us


微信:OALib Journal