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PeerJ  2015 

Error estimates for the analysis of differential expression from RNA-seq count data

DOI: 10.7717/peerj.576

Keywords: RNA-seq,Differential expression analysis,False discovery rates

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Background. A number of algorithms exist for analysing RNA-sequencing data to infer profiles of differential gene expression. Problems inherent in building algorithms around statistical models of over dispersed count data are formidable and frequently lead to non-uniform p-value distributions for null-hypothesis data and to inaccurate estimates of false discovery rates (FDRs). This can lead to an inaccurate measure of significance and loss of power to detect differential expression.


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