A normal phase high performance thin layer chromatography (HPTLC) method has been developed and validated for simultaneous estimation of four components, namely, alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from two medicinally important plants, Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. In Ayurveda, both plants have been reported to possess immunomodulatory activity. Chromatographic separation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. was performed on TLC aluminium plates precoated with silica gel using a suitable mobile phase. The densitometric scanning was done after derivatization at λ = 580？nm for α-amyrin, β-sitosterol, and lupeol, and at 366？nm for n-triacontane. The developed HPTLC method has been validated and used for simultaneous quantitation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. The developed HPTLC method is simple, rapid, and precise and can be used for routine quality control. 1. Introduction Herbal medicines have been used since ages to treat various ailments. Ayurveda is an Indian traditional system of medicine used since ancient times. It has a huge list of herbs used in various forms for treatment of different disease conditions. Owing to the medicinal properties attributed to herbal drugs, it is necessary to maintain their quality and purity, thereby justifying their acceptability in modern system of medicine. Standardisation of these herbal drugs is a challenge to the entire scientific fraternity. However, due to lack of suitable quality control and quality assurance standards for herbal drugs, it becomes difficult to ensure uniformity of their composition which in turn affects the efficacy of their final products. Analytical tools are important for qualitative, semiquantitative, and quantitative phytochemical analysis of herbal drugs and formulations. Chromatographic techniques such as high performance liquid chromatography (HPLC), high performance thin layer chromatography (HPTLC), and gas chromatography (GC) are used to efficiently determine the quality of the herbs by developing fingerprints and estimation of biomarkers. Among the wide choice of chromatographic techniques, HPTLC is a simple, fast, and accurate technique for use, making it advantageous over others for quick assessment of a number of samples simultaneously . In the present research work, HPTLC
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