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Macrophage Phagocytosis Abilities Measured with Flow Cytometry Method

DOI: 10.12677/is.2014.21001, PP. 1-4

Keywords: 流式细胞仪;巨噬细胞;非调理素吞噬
Flow Cytometry
, Macrophages, Non-Opsonic Phagocytosis

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Macrophage is an important component of innate immunity, which plays a great role in anti-infection immunity. The accurate evaluation of non-opsonic phagocytosis of macrophages is often used as an important indicator of the non-specific immune responses when bacterial infection occurs. In the present study, the non-opsonic phagocytosis of macrophages in resident or inflammatory infiltrating condition was quantificationally detected by using flow cytometry method combined with bacterial counts. This method will contribute to the quantitative evaluation for the ability of macrophage phagocytosis and the accurate response to macrophage immunity in a variety of physiological and pathological conditions.


[1]  Yamamoto, M., Okuyama, M., Ma, J., Kimura, T., Kamiyama, N., Saiga, H., et al. (2012) A cluster of interferon-gamma-inducible p65 GTPases plays a critical role in host defense against Toxoplasma gondii. Immunity, 37, 302-313.
[2]  Potian, J., Rafi, W., Bhatt, K., Mcbride, A., Gause, W. and Salgame, P. (2012) Preexisting helminth infection induces inhibition of innate pulmonary anti-tuberculosis defense by engaging the IL-4 receptor pathway. The Journal of Experimental Medicine, 208, 1863-1874.
[3]  Cramer, R., Rivera, A and Hohl, T. (2011) Immune responses against Aspergillus fumigatus: What have we learned? Current Opinion in Infectious Diseases, 24, 315-322.
[4]  Kim, B., Shenoy, A., Kumar, P., Das, R, Tiwari, S. and Macmicking, J. (2012) A family of IFN-gamma-inducible 65-kD GTPases protects against bacterial infection. Science, 332, 717721.
[5]  Jantsch, J., Chikkaballi, D. and Hensel, M. (2011) Cellular aspects of immunity to intracellular Salmonella enterica. Immunological Reviews, 240, 185-195.
[6]  Akira, S., Misawa, T., Satoh, T. and Saitoh, T. (2013) Macrophages control innate inflammation. Diabetes, Obesity and Metabolism, 15, 10-18.
[7]  Liu, G., Ma, H., Jiang, L., Peng, J. and Zhao, Y. (2007) The immunity of splenic and peritoneal F4/80(+) resident macro-phages in mouse mixed allogeneic chimeras. Journal of molecular medicine, 85, 1125-1135.
[8]  Dai, W., Kohler, G. and Brombacher, F. (1997) Both innate and acquired immunity to Listeria monocytogenes infection are increased in IL-10-deficient mice. The Journal of Immunology, 158, 2259-2267.
[9]  Bourgeois, C., Majer, O., Frohner, I., Le-siak-Markowicz, I., Hildering, K., Glaser, W., et al. (2010) Conventional dendritic cells mount a type I IFN response against Candida spp. requiring novel phagosomal TLR7-mediated IFN-beta signaling. The Journal of Immunology, 186, 3104-3112.


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