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流式细胞仪方法定量评价巨噬细胞非调理素吞噬能力
Macrophage Phagocytosis Abilities Measured with Flow Cytometry Method

DOI: 10.12677/is.2014.21001, PP. 1-4

Keywords: 流式细胞仪;巨噬细胞;非调理素吞噬
Flow Cytometry
, Macrophages, Non-Opsonic Phagocytosis

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Abstract:

巨噬细胞是重要的天然免疫细胞,在抗细菌感染等免疫应答中发挥重要调控作用。当细菌等病原微生物感染机体时,准确的定量评价巨噬细胞的非调理素吞噬能力,常常被作为评价机体非特异免疫应答能力的重要指标。本文采用流式细胞仪结合细菌计数的方法,定量的检测了定居巨噬细胞和炎症浸润的巨噬细胞对细菌的非调理素吞噬能力变化。该研究方法将有助于定量评价在免疫应答中巨噬细胞的吞噬能力变化,以更准确反映在各种生理或病理条件下巨噬细胞的免疫应答状态。
Macrophage is an important component of innate immunity, which plays a great role in anti-infection immunity. The accurate evaluation of non-opsonic phagocytosis of macrophages is often used as an important indicator of the non-specific immune responses when bacterial infection occurs. In the present study, the non-opsonic phagocytosis of macrophages in resident or inflammatory infiltrating condition was quantificationally detected by using flow cytometry method combined with bacterial counts. This method will contribute to the quantitative evaluation for the ability of macrophage phagocytosis and the accurate response to macrophage immunity in a variety of physiological and pathological conditions.

References

[1]  Yamamoto, M., Okuyama, M., Ma, J., Kimura, T., Kamiyama, N., Saiga, H., et al. (2012) A cluster of interferon-gamma-inducible p65 GTPases plays a critical role in host defense against Toxoplasma gondii. Immunity, 37, 302-313.
[2]  Potian, J., Rafi, W., Bhatt, K., Mcbride, A., Gause, W. and Salgame, P. (2012) Preexisting helminth infection induces inhibition of innate pulmonary anti-tuberculosis defense by engaging the IL-4 receptor pathway. The Journal of Experimental Medicine, 208, 1863-1874.
[3]  Cramer, R., Rivera, A and Hohl, T. (2011) Immune responses against Aspergillus fumigatus: What have we learned? Current Opinion in Infectious Diseases, 24, 315-322.
[4]  Kim, B., Shenoy, A., Kumar, P., Das, R, Tiwari, S. and Macmicking, J. (2012) A family of IFN-gamma-inducible 65-kD GTPases protects against bacterial infection. Science, 332, 717721.
[5]  Jantsch, J., Chikkaballi, D. and Hensel, M. (2011) Cellular aspects of immunity to intracellular Salmonella enterica. Immunological Reviews, 240, 185-195.
[6]  Akira, S., Misawa, T., Satoh, T. and Saitoh, T. (2013) Macrophages control innate inflammation. Diabetes, Obesity and Metabolism, 15, 10-18.
[7]  Liu, G., Ma, H., Jiang, L., Peng, J. and Zhao, Y. (2007) The immunity of splenic and peritoneal F4/80(+) resident macro-phages in mouse mixed allogeneic chimeras. Journal of molecular medicine, 85, 1125-1135.
[8]  Dai, W., Kohler, G. and Brombacher, F. (1997) Both innate and acquired immunity to Listeria monocytogenes infection are increased in IL-10-deficient mice. The Journal of Immunology, 158, 2259-2267.
[9]  Bourgeois, C., Majer, O., Frohner, I., Le-siak-Markowicz, I., Hildering, K., Glaser, W., et al. (2010) Conventional dendritic cells mount a type I IFN response against Candida spp. requiring novel phagosomal TLR7-mediated IFN-beta signaling. The Journal of Immunology, 186, 3104-3112.

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