Macrophage is an important component of innate immunity, which plays a great role in anti-infection immunity. The accurate evaluation of non-opsonic phagocytosis of macrophages is often used as an important indicator of the non-specific immune responses when bacterial infection occurs. In the present study, the non-opsonic phagocytosis of macrophages in resident or inflammatory infiltrating condition was quantificationally detected by using flow cytometry method combined with bacterial counts. This method will contribute to the quantitative evaluation for the ability of macrophage phagocytosis and the accurate response to macrophage immunity in a variety of physiological and pathological conditions.
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