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Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells

DOI: 10.4236/gep.2014.22018, PP. 129-133

Keywords: Cadmium Chloride, Human Bronchial Epithelial Cells, Malignant Transformation, Telomerase Reverse Transcriptase

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The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithelial (16HBE) cells. Fluorescence quantitative PCR (FQ-PCR) and Western blot analyses were performed to detect the hTERT mRNA and protein expressions in normal 16HBE cells, cadmium chloride-transformed 16HBE cells, and tumorigenic cells from nude mice inoculated with cadmium chloride-transformed 16HBE cells. Under the inner standard of GAPDH, the hTERT mRNA expression was significantly higher at different stages of malignant transformation (cadmium chloride-transformed 16HBE cells at passages 15 and 35 and tumorigenic cells from nude mice) than in normal 16HBE cells, and increased with the development of malignancy (P < 0.01). In addition, hTERT protein expression increased with the development of malignancy. These findings demonstrate that hTERT expression is related to cadmium chlorideinduced malignant transformation. Cadmium chloride-induced malignant transformation is involved in changes in the hTERT activity, and might be an early event in cadmium chloride-induced malignant transformation.


[1]  Harrington, L. (2003). Biochemical Aspects of Telomerase Function. Cancer Letters, 194, 139-154.
[2]  IARC (1993). Beryllium, Cadmium, Mercury and Exposures in the Glass Manufacturing Industry. International Agency for Research on Cancer, 58, 119-238.
[3]  Jetten, A. M. (1989). Multistep Process of Squamous Differentiation in Tracheobronchial Epithelial Cells in Vitro: Analogy with Epidermal Differentiation. Environmental Health Perspectives, 80, 149-160.
[4]  Koizumi, S., & Yamada, H. (2003). DNA Microarray Analysis of Altered Gene Expression in Cadmium-Exposed Human Cells. Journal of Occupational Health, 45, 331-334.
[5]  Lei, Y. X., Wei, L., Wang, M., Wu, G. R., & Li, M. (2008). Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride. Biomedical and Environmental Sciences, 21, 332-338.
[6]  Li, Z., Ma, Z., van der Kuijp, T. J., Yuan, Z., & Huang, L. (2014). A Review of Soil Heavy Metal Pollution from Mines in China: Pollution and Health Risk Assessment. Science of the Total Environment, 468-469, 843-853.
[7]  Masutomi, K., Yu, E. Y., Khurts, S., Ben-Porath, I., Currier, J. L., Metz, G. B., Brooks, M. W., Kaneko, S., Murakami, S., DeCaprio, J. A., Weinberg, R. A., Stewart, S. A., & Hahn, W. C. (2003). Telomerase Maintains Telomere Structure in Normal Human Cells. Cell, 114, 241-253.
[8]  Neidle, S., & Parkinson, G. N. (2003). The Structure of Telomeric DNA. Current Opinion in Structural Biology, 13, 275- 283.
[9]  Ross, R. A., Biedler, J. L., & Spengler, B. A. (2003). A Role for Distinct Cell Types in Determining Malignancy in Human Neuroblastoma Cell Lines and Tumors. Cancer Letters, 197, 35-39.
[10]  Sara, K., & Victoria, L. (2001). Positive and Negative Regulation of Telomerase Access to the Telomere. Cell Science, 113, 3357-3364
[11]  Wei, L., Lei, Y. X., Wu, L., Wang, M., Lu, Q., & He, C. C. (2012). Alterations in the Expression of Translation Factors as Molecular Markers in Cadmium-Exposed Workers. Biomarkers, 17, 78-84.
[12]  Zhou, Z. H., Lei, Y. X., & Wang, C. X. (2012). Analysis of Aberrant Methylation in DNA Repair Genes during Malignant Transformation of Human Bronchial Epithelial Cells Induced by Cadmium. Toxicological Sciences, 125, 412-417.


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