全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
PLOS ONE  2014 

Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking

DOI: 10.1371/journal.pone.0102037

Full-Text   Cite this paper   Add to My Lib

Abstract:

Cigarette smoking is the main risk factor for COPD (Chronic Obstructive Pulmonary Disease), yet only a subset of smokers develops COPD. Family members of patients with severe early-onset COPD have an increased risk to develop COPD and are therefore defined as “susceptible individuals”. Here we perform unbiased analyses of proteomic profiles to assess how “susceptible individuals” differ from age-matched “non-susceptible individuals” in response to cigarette smoking. Epithelial lining fluid (ELF) was collected at baseline and 24 hours after smoking 3 cigarettes in young individuals susceptible or non-susceptible to develop COPD and older subjects with established COPD. Controls at baseline were older healthy smoking and non-smoking individuals. Five samples per group were pooled and analysed by stable isotope labelling (iTRAQ) in duplicate. Six proteins were selected and validated by ELISA or immunohistochemistry. After smoking, 23 proteins increased or decreased in young susceptible individuals, 7 in young non-susceptible individuals, and 13 in COPD in the first experiment; 23 proteins increased or decreased in young susceptible individuals, 32 in young non-susceptible individuals, and 11 in COPD in the second experiment. SerpinB3 and Uteroglobin decreased after acute smoke exposure in young non-susceptible individuals exclusively, whereas Peroxiredoxin I, S100A9, S100A8, ALDH3A1 (Aldehyde dehydrogenase 3A1) decreased both in young susceptible and non-susceptible individuals, changes being significantly different between groups for Uteroglobin with iTRAQ and for Serpin B3 with iTRAQ and ELISA measures. Peroxiredoxin I, SerpinB3 and ALDH3A1 increased in COPD patients after smoking. We conclude that smoking induces a differential protein response in ELF of susceptible and non-susceptible young individuals, which differs from patients with established COPD. This is the first study applying unbiased proteomic profiling to unravel the underlying mechanisms that induce COPD. Our data suggest that SerpinB3 and Uteroglobin could be interesting proteins in understanding the processes leading to COPD.

References

[1]  Siafakas NM, Vermeire P, Pride NB, Paoletti P, Gibson J, et al. (1995) Optimal assessment and management of chronic obstructive pulmonary disease (COPD). The European Respiratory Society Task Force. Eur Respir J 8: 1398–1420. doi: 10.1183/09031936.95.08081398
[2]  Sabroe I, Parker LC, Calverley PM, Dower SK, Whyte MK (2007) Pathological networking: a new approach to understanding COPD. Thorax 62: 733–738. doi: 10.1136/thx.2007.077768
[3]  O'Neil SE, Lundback B, Lotvall J (2011) Proteomics in asthma and COPD phenotypes and endotypes for biomarker discovery and improved understanding of disease entities. J Proteomics 75: 192–201 S1874-3919(11)00494-5 [pii];10.1016/j.jprot.2011.10.008 [doi].
[4]  Chen H, Wang D, Bai C, Wang X (2010) Proteomics-based biomarkers in chronic obstructive pulmonary disease. J Proteome Res 9: 2798–2808 10.1021/pr100063r [doi].
[5]  Nicholas BL, O'Connor CD, Djukanovic R (2009) From proteomics to prescription-the search for COPD biomarkers. COPD 6: 298–303 10.1080/15412550903049140 [pii]. doi: 10.1080/15412550903049140
[6]  Plymoth A, Lofdahl CG, Ekberg-Jansson A, Dahlback M, Broberg P, et al. (2007) Protein expression patterns associated with progression of chronic obstructive pulmonary disease in bronchoalveolar lavage of smokers. Clin Chem 53: 636–644 clinchem.2006.076075 [pii];10.1373/clinchem.2006.076075 [doi].
[7]  Plymoth A, Yang Z, Lofdahl CG, Ekberg-Jansson A, Dahlback M, et al. (2006) Rapid proteome analysis of bronchoalveolar lavage samples of lifelong smokers and never-smokers by micro-scale liquid chromatography and mass spectrometry. Clin Chem 52: 671–679 clinchem.2005.060715 [pii];10.1373/clinchem.2005.060715 [doi].
[8]  Tu C, Mammen MJ, Li J, Shen X, Jiang X, et al. (2014) Large-scale, ion-current-based proteomics investigation of bronchoalveolar lavage fluid in chronic obstructive pulmonary disease patients. J Proteome Res 13: 627–639 10.1021/pr4007602 [doi].
[9]  Pastor MD, Nogal A, Molina-Pinelo S, Melendez R, Romero-Romero B, et al. (2013) Identification of oxidative stress related proteins as biomarkers for lung cancer and chronic obstructive pulmonary disease in bronchoalveolar lavage. Int J Mol Sci 14: 3440–3455 ijms14023440 [pii];10.3390/ijms14023440 [doi].
[10]  Gray RD, MacGregor G, Noble D, Imrie M, Dewar M, et al. (2008) Sputum proteomics in inflammatory and suppurative respiratory diseases. Am J Respir Crit Care Med 178: 444–452 200703-409OC [pii];10.1164/rccm.200703-409OC [doi].
[11]  Nicholas B, Skipp P, Mould R, Rennard S, Davies DE, et al. (2006) Shotgun proteomic analysis of human-induced sputum. Proteomics 6: 4390–4401 10.1002/pmic.200600011 [doi].
[12]  Casado B, Iadarola P, Pannell LK, Luisetti M, Corsico A, et al. (2007) Protein expression in sputum of smokers and chronic obstructive pulmonary disease patients: a pilot study by CapLC-ESI-Q-TOF. J Proteome Res 6: 4615–4623 10.1021/pr070440q [doi].
[13]  Ohlmeier S, Mazur W, Linja-Aho A, Louhelainen N, Ronty M, et al. (2012) Sputum proteomics identifies elevated PIGR levels in smokers and mild-to-moderate COPD. J Proteome Res 11: 599–608 10.1021/pr2006395 [doi].
[14]  Fumagalli M, Ferrari F, Luisetti M, Stolk J, Hiemstra PS, et al. (2012) Profiling the Proteome of Exhaled Breath Condensate in Healthy Smokers and COPD Patients by LC-MS/MS. Int J Mol Sci 13: 13894–13910 ijms131113894 [pii];10.3390/ijms131113894 [doi].
[15]  Tzortzaki EG, Siafakas NM (2009) A hypothesis for the initiation of COPD. Eur Respir J 34: 310–315. doi: 10.1183/09031936.00067008
[16]  van der Vaart H, Postma DS, Timens W, ten Hacken NH (2004) Acute effects of cigarette smoke on inflammation and oxidative stress: a review. Thorax 59: 713–721. doi: 10.1136/thx.2003.012468
[17]  van der VaartH, Postma DS, Timens W, Hylkema MN, Willemse BW, et al. (2005) Acute effects of cigarette smoking on inflammation in healthy intermittent smokers. Respir Res 6: 22.
[18]  Siafakas NM, Tzortzaki EG (2002) Few smokers develop COPD. Why? Respir Med 96: 615–624. doi: 10.1053/rmed.2002.1318
[19]  Silverman EK, Chapman HA, Drazen JM, Weiss ST, Rosner B, et al. (1998) Genetic epidemiology of severe, early-onset chronic obstructive pulmonary disease. Risk to relatives for airflow obstruction and chronic bronchitis. Am J Respir Crit Care Med 157: 1770–1778. doi: 10.1164/ajrccm.157.6.9706014
[20]  Kipnis E, Hansen K, Sawa T, Moriyama K, Zurawel A, et al. (2008) Proteomic analysis of undiluted lung epithelial lining fluid. Chest 134: 338–345 134/2/338 [pii];10.1378/chest.07-1643 [doi].
[21]  Lo Tam Loi AT, Hoonhorst SJ, Franciosi L, Bischoff R, Hoffmann RF, et al. (2013) Acute and chronic inflammatory responses induced by smoking in individuals susceptible and non-susceptible to development of COPD: from specific disease phenotyping towards novel therapy. Protocol of a cross-sectional study. BMJ Open 3. doi: 10.1136/bmjopen-2012-002178
[22]  Du R, I, Barber PV, Goldring J, Lewis RA, Mandal S, et al. (2011) British Thoracic Society guideline for advanced diagnostic and therapeutic flexible bronchoscopy in adults. Thorax 66 Suppl 3: iii1–21. doi: 10.1136/thoraxjnl-2011-200713
[23]  Franciosi L, Govorukhina N, Ten HN, Postma D, Bischoff R (2011) Proteomics of epithelial lining fluid obtained by bronchoscopic microprobe sampling. Methods Mol Biol 790: 17–28. doi: 10.1007/978-1-61779-319-6_2
[24]  Choe L, D'Ascenzo M, Relkin NR, Pappin D, Ross P, et al. (2007) 8-plex quantitation of changes in cerebrospinal fluid protein expression in subjects undergoing intravenous immunoglobulin treatment for Alzheimer's disease. Proteomics 7: 3651–3660. doi: 10.1002/pmic.200700316
[25]  Steen A, Wiederhold E, Gandhi T, Breitling R, Slotboom DJ (2011) Physiological adaptation of the bacterium Lactococcus lactis in response to the production of human CFTR. Mol Cell Proteomics 10 M000052MCP200. doi: 10.1074/mcp.m000052-mcp200
[26]  Dijkstra A, Postma DS, Noordhoek JA, Lodewijk ME, Kauffman HF, et al. (2009) Expression of ADAMs ("a disintegrin and metalloprotease") in the human lung. Virchows Arch 454: 441–449. doi: 10.1007/s00428-009-0748-4
[27]  Lunardi F, Villano G, Perissinotto E, Agostini C, Rea F, et al. (2011) Overexpression of SERPIN B3 promotes epithelial proliferation and lung fibrosis in mice. Lab Invest 91: 945–954. doi: 10.1038/labinvest.2011.1
[28]  de Torre C, Ying SX, Munson PJ, Meduri GU, Suffredini AF (2006) Proteomic analysis of inflammatory biomarkers in bronchoalveolar lavage. Proteomics 6: 3949–3957. doi: 10.1002/pmic.200500693
[29]  Ehrchen JM, Sunderkotter C, Foell D, Vogl T, Roth J (2009) The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer. J Leukoc Biol 86: 557–566. doi: 10.1189/jlb.1008647
[30]  Lorenz E, Muhlebach MS, Tessier PA, Alexis NE, Duncan HR, et al. (2008) Different expression ratio of S100A8/A9 and S100A12 in acute and chronic lung diseases. Respir Med 102: 567–573. doi: 10.1016/j.rmed.2007.11.011
[31]  Lakind JS, Holgate ST, Ownby DR, Mansur AH, Helms PJ, et al. (2007) A critical review of the use of Clara cell secretory protein (CC16) as a biomarker of acute or chronic pulmonary effects. Biomarkers 12: 445–467. doi: 10.1080/13547500701359327
[32]  Kwon HS, Bae YJ, Moon KA, Lee YS, Lee T, et al. (2012) Hyperoxidized peroxiredoxins in peripheral blood mononuclear cells of asthma patients is associated with asthma severity. Life Sci 90: 502–508. doi: 10.1016/j.lfs.2012.01.003
[33]  van der TM, Smit-de Vries MP, Slebos DJ, de Bruin HG, Abello N, et al. (2007) Cigarette smoke irreversibly modifies glutathione in airway epithelial cells. Am J Physiol Lung Cell Mol Physiol 293: L1156–L1162. doi: 10.1152/ajplung.00081.2007
[34]  Schick C, Pemberton PA, Shi GP, Kamachi Y, Cataltepe S, et al. (1998) Cross-class inhibition of the cysteine proteinases cathepsins K, L, and S by the serpin squamous cell carcinoma antigen 1: a kinetic analysis. Biochemistry 37: 5258–5266. doi: 10.1021/bi972521d
[35]  Landi C, Bargagli E, Magi B, Prasse A, Muller-Quernheim J, et al. (2011) Proteome analysis of bronchoalveolar lavage in pulmonary langerhans cell histiocytosis. J Clin Bioinforma 1: 31. doi: 10.1186/2043-9113-1-31
[36]  van Miert E, Dumont X, Bernard A (2005) CC16 as a marker of lung epithelial hyperpermeability in an acute model of rats exposed to mainstream cigarette smoke. Toxicol Lett 159: 115–123. doi: 10.1016/j.toxlet.2005.05.007
[37]  Lumsden AB, McLean A, Lamb D (1984) Goblet and Clara cells of human distal airways: evidence for smoking induced changes in their numbers. Thorax 39: 844–849. doi: 10.1136/thx.39.11.844
[38]  Shijubo N, Itoh Y, Yamaguchi T, Shibuya Y, Morita Y, et al. (1997) Serum and BAL Clara cell 10 kDa protein (CC10) levels and CC10-positive bronchiolar cells are decreased in smokers. Eur Respir J 10: 1108–1114. doi: 10.1183/09031936.97.10051108
[39]  Shijubo N, Honda Y, Itoh Y, Yamaguchi T, Kuroki Y, et al. (1998) BAL surfactant protein A and Clara cell 10-kDa protein levels in healthy subjects. Lung 176: 257–265. doi: 10.1007/pl00007608
[40]  Merkel D, Rist W, Seither P, Weith A, Lenter MC (2005) Proteomic study of human bronchoalveolar lavage fluids from smokers with chronic obstructive pulmonary disease by combining surface-enhanced laser desorption/ionization-mass spectrometry profiling with mass spectrometric protein identification. Proteomics 5: 2972–2980. doi: 10.1002/pmic.200401180
[41]  Bernard A, Marchandise FX, Depelchin S, Lauwerys R, Sibille Y (1992) Clara cell protein in serum and bronchoalveolar lavage. Eur Respir J 5: 1231–1238.
[42]  Lomas DA, Silverman EK, Edwards LD, Miller BE, Coxson HO, et al. (2008) Evaluation of serum CC-16 as a biomarker for COPD in the ECLIPSE cohort. Thorax 63: 1058–1063. doi: 10.1136/thx.2008.102574
[43]  Braido F, Riccio AM, Guerra L, Gamalero C, Zolezzi A, et al. (2007) Clara cell 16 protein in COPD sputum: a marker of small airways damage? Respir Med 101: 2119–2124. doi: 10.1016/j.rmed.2007.05.023
[44]  Celedon JC, Speizer FE, Drazen JM, Weiss ST, Campbell EJ, et al. (1999) Bronchodilator responsiveness and serum total IgE levels in families of probands with severe early-onset COPD. Eur Respir J 14: 1009–1014. doi: 10.1183/09031936.99.14510099
[45]  McCloskey SC, Patel BD, Hinchliffe SJ, Reid ED, Wareham NJ, et al. (2001) Siblings of patients with severe chronic obstructive pulmonary disease have a significant risk of airflow obstruction. Am J Respir Crit Care Med 164: 1419–1424. doi: 10.1164/ajrccm.164.8.2105002
[46]  Patel BD, Coxson HO, Pillai SG, Agusti AG, Calverley PM, et al. (2008) Airway wall thickening and emphysema show independent familial aggregation in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 178: 500–505. doi: 10.1164/rccm.200801-059oc

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133