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Foods  2013 

Health Risk Assessment of Pesticide Residues via Dietary Intake of Market Vegetables from Dhaka, Bangladesh

DOI: 10.3390/foods2010064

Keywords: pesticide residues, vegetables, health risk, health hazard, food safety, HRI, HPLC-PDA

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The present study was designed to assess the health risk of pesticide residues via dietary intake of vegetables collected from four top agro-based markets of Dhaka, Bangladesh. High performance liquid chromatography with a photo diode array detector (HPLC-PDA) was used to determine six organophosphorus (chlorpyrifos, fenitrothion, parathion, ethion, acephate, fenthion), two carbamate (carbaryl and carbofuran) and one pyrethroid (cypermethrin) pesticide residues in twelve samples of three common vegetables (tomato, lady’s finger and brinjal). Pesticide residues ranged from below detectable limit (<0.01) to 0.36 mg/kg. Acephate, chlorpyrifos, ethion, carbaryl and cypermethrin were detected in only one sample, while co-occurrence occurred twice for fenitrothion and parathion. Apart from chlorpyrifos in tomato and cypermethrin in brinjal, all pesticide residues exceeded the maximum residue limit (MRL). Hazard risk index (HRI) for ethion (10.12) and carbaryl (1.09) was found in lady’s finger and tomato, respectively. Rest of the pesticide residues were classified as not a health risk. A continuous monitoring and strict regulation should be enforced regarding control of pesticide residues in vegetables and other food commodities.


[1]  Bempah, C.K.; Donkor, A.; Yeboah, P.O.; Dubey, B.; Osei-Fosu, P. A preliminary assessment of consumer’s exposure to organochlorine pesticides in fruits and vegetables and the potential health risk in Accra Metropolis, Ghana. Food Chem. 2011, 128, 1058–1065.
[2]  Ntow, W.J.; Gijzen, H.J.; Drechsel, P. Farmer perceptions and pesticide use practices in vegetable production in Ghana. Pest Manag. Sci. 2006, 62, 356–365, doi:10.1002/ps.1178.
[3]  Mansour, S.A.; Belal, M.H.; Abou-Arab, A.A.; Gad, M.F. Monitoring of pesticide and heavy metals in cucumber fruits produced from different farming system. Chemosphere 2009, 75, 601–609, doi:10.1016/j.chemosphere.2009.01.058.
[4]  Hayat, K.; Ashfaq, M.; Ashfaq, U.; Saleem, M.A. Determination of pesticide residues in blood samples of villagers involved in pesticide application at District Vehari (Punjab), Pakistan. African J. Environ. Sci. Tech. 2011, 4, 666–684.
[5]  Choi, S.M.; Yoo, S.D.; Lee, B.M. Toxicological characteristics of endocrine disrupting chemicals: Developmental toxicity, carcinogenicity, and mutagenicity. J. Toxicol. Environ. Health B Crit. Rev. 2004, 7, 1–24.
[6]  Galloway, T.; Handy, R. Immunotoxicity of organophosphorous pesticides. Ecotoxicology 2003, 12, 345–363, doi:10.1023/A:1022579416322.
[7]  Rauh, V.A.; Garfinkel, R.; Perera, F.P.; Andrews, H.F.; Hoepner, L.; Barr, D.B.; Whitehead, R.; Tang, D.; Whyatt, R.W. Impact of prenatal chlorpyrifos exposure on neurodevelopment in the first 3 years of life among inner-city children. Pediatrics 2006, 118, 1845–1859.
[8]  EPA (US Environmental Protection Agency). Health and Environmental Effects Profile for Carbaryl; Environmental Criteria and Assessment Office, Office of Health and Environmental Assessment, Office of Research and Development: Cincinnati, OH, USA, 1984.
[9]  EPA (US Environmental Protection Agency). Integrated Risk Information System (IRIS) on Carbaryl; National Center for Environmental Assessment, Office of Research and Development: Washington, DC, USA, 1999.
[10]  Khaniki, G.R. Chemical contaminants in milk and public health concerns: A review. Int. J. Dairy Sci. 2007, 2, 104–115, doi:10.3923/ijds.2007.104.115.
[11]  WHO (World Health Organization in collaboration with UNEP). Public Health Impact of Pesticides Used in Agriculture; WHO: Geneva, Switzerland, 1990; pp. 1–129.
[12]  Bhanti, M.; Shukla, G.; Taneja, A. Contamination levels of organochlorine pesticides andfarmers’ knowledge, perception, practices in rural India—A case study. Bull. Environ. Conta. Toxicol. 2004, 73, 787–793.
[13]  FAO/WHO (Food and Agriculture Organization/World Health Organization). Food Standards Programme. In Proccedings of Codex Alimentarius Commission. Twenty-Seventh Session, Geneva, Switzerland, 28 June–03 July 2004; pp. 1–103.
[14]  WHO (World Health Organization). Guidelines for Predicting Dietary Intake of Pesticide Residues (Revised). Available online: (access on 05 February 2013).
[15]  Darko, G.; Akoto, O. Dietary intake of organophosphorus pesticide residuesthrough vegetables from Kumasi, Ghana. Food Chem. Toxicol. 2008, 46, 3703–3706, doi:10.1016/j.fct.2008.09.049.
[16]  Chowdhury, M.A.Z.; Banik, S.; Uddin, B.; Moniruzzaman, M.; Karim, N.; Gan, S.H. Organophosphorus and carbamate pesticide residues detected in water samples collected from paddy and vegetable fields of the Savar and Dhamrai Upazilas in Bangladesh. Int. J. Environ. Res. Pub. Health 2012, 9, 3318–3329, doi:10.3390/ijerph9093318.
[17]  Chowdhury, A.Z.; Jahan, S.A.; Islam, M.N.; Moniruzzaman, M.; Alam, M.K.; Zaman, M.A.; Karim, N.; Gan, S.H. Occurrence of organophosphorus and carbamate pesticide residues in surface water samples from the Rangpur district of Bangladesh. Bull. Environ. Conta. Toxicol. 2012, 89, 202–207.
[18]  Businelli, A.; Vischetti, C.; Coletti, A. Validation of Koc approach for modeling the fate of some herbicides in Italian soil. Fresenius Environ. Bull. 1992, 1, 583–588.
[19]  Amoah, P.; Drechsel, P.; Abaidoo, R.C.; Ntow, W.J. Pesticide and pathogen contamination of vegetables in Ghana’s urban markets. Arch. Environ. Conta. Toxicol. 2006, 50, 1–6, doi:10.1007/s00244-004-0054-8.
[20]  Solecki, R.; Davies, L.; Dellarco, V.; Dewhurst, I.; Raaij, M.V.; Tritscher, A. Guidance on setting of acute reference dose (ARfD) for pesticides. Food Chem. Toxicol. 2005, 43, 1569–1593, doi:10.1016/j.fct.2005.04.005.
[21]  SAC (Standardization Administration of China). Fresh Fruits and Vegetables-Sampling. GB/T 8855-2008; The Standardization Administration of China: Beijing, China, 2008; pp. 1–8.
[22]  Fardous, Z.; Islam, M.N.; Hoque, S.M.; Chowdhury, M.A.Z.; Rahman, M.A. Determination of some selected pesticide residues in tomato from different locations of Bangladesh. Int. J. Sustain. Agri. Tech. 2007, 3, 4–7.
[23]  Rahman, M.A.; Chowdhury, A.Z.; Moniruzzaman, M.; Gan, S.H.; Islam, M.N.; Fardous, Z.; Alam, M.K. Pesticide residues in tobacco leaves from the Kushtia district in Bangladesh. Bull. Environ. Contam. Toxicol. 2012, 89, 658–663.
[24]  FAO (Food and Agriculture Organization). Submission and Evaluation of Pesticide Residues Data for the Estimation of Maximum Residue Levels in Food and Feed; FAO: Rome, Italy, 2002; pp. 1–279.
[25]  Risk Management and Food Safety. Report of a Joint FAO/WHO Consultation. Rome, Itlay, 27 to 31 January 1997. Available online: (accessed on 05 February 2013).
[26]  Wang, X.; Sato, T.; Xing, B.; Tao, S. Health risks of heavy metals to the general public in Tianjin, China via consumption of vegetables and fish. Sci. Total Environ. 2005, 350, 28–37, doi:10.1016/j.scitotenv.2004.09.044.
[27]  EFSA Scientific Colloquium Summary Report. Cumulative Risk Assessment of Pesticides to Human Health: The Way Forward. Available online: (access on 05 February 2013).
[28]  Sinha, S.N.; Rao, M.V.V.; Vasudev, K. Distribution of pesticides in different commonly used vegetables from Hyderabad, India. Food Res. Int. 2012, 45, 161–169, doi:10.1016/j.foodres.2011.09.028.
[29]  Farag, R.S.; Latif, A.M.S.; El-Gawad, A.E.; Dogheim, S.M. Monitoring of pesticide residues in some Egyptian herbs, fruits and vegetables. Int. Food Res. J. 2011, 18, 659–665.
[30]  Chen, C.; Qian, Y.; Chen, Q.; Tao, C.; Li, C.; Li, Y. Evaluation of pesticide residues in fruits and vegetables from Xiamen, China. Food Cont. 2011, 22, 1114–1120, doi:10.1016/j.foodcont.2011.01.007.
[31]  Latif, Y.; Sherazi, S.T.H.; Bhanger, M.I. Assessment of pesticide residues in commonly used vegetables in Hyderabad, Pakistan. Ecotoxicol. Environ. Safety 2011, 74, 2299–2303, doi:10.1016/j.ecoenv.2011.07.030.
[32]  Osman, K.A.; Al-Humaid, A.I.; Al-Rehiayani, S.M.; Al-Redhaiman, K.N. Estimated daily intake of pesticide residues exposure by vegetables grown in greenhouses in Al-Qassim region, Saudi Arabia. Food Cont. 2011, 22, 947–953, doi:10.1016/j.foodcont.2010.11.031.
[33]  Dhas, S.; Srivastava, M. An assessment of carbaryl residues on brinjal crop in an agricultural field in Bikaner, Rajasthan, India. Asian J. Agri. Sci. 2010, 2, 15–17.
[34]  Chun, O.K.; Kang, H.G. Estimation of risks of pesticide exposure, by food intake, to Koreans. Food Chem. Toxicol. 2003, 41, 1063–1076, doi:10.1016/S0278-6915(03)00044-9.
[35]  Chandra, S.; Mahindrakar, A.N.; Shinde, L.P. Determination of cypermethrin and chlorpyrifos in vegetables by GC-ECD. Int. J. ChemTech Res. 2010, 2, 908–911.
[36]  EU MRLs in View of the First Establishment of Annex II to Regulation (EC) N. 396/2005. Available online: (accessed on 10 December 2012).
[37]  Tanabe, S.; Prudente, M.S.; Kan-atireklap, S.; Subramanian, A. Mussel watch: Marine pollution monitoring of butyltins and organochlorines in coastal waters of Thailand, Philippines and India. Ocean Coast. Manag. 2000, 43, 819–839, doi:10.1016/S0964-5691(00)00060-0.
[38]  Pesticides. Food Monitoring, 1998–2003. Part 2. Available online: (accessed on 05 February 2013).
[39]  AG (Australian Government). Acceptable Daily Intakes for Agricultural and Veterinary Chemicals; Office of Chemical Safety, Department of Health and Ageing: Canberra, Australia, 2005; pp. 1–115.


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