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Foods  2013 

Molecular Features of Wheat Endosperm Arabinoxylan Inclusion in Functional Bread

DOI: 10.3390/foods2020225

Keywords: arabinoxylans, endoxylanase, functional bread, extractability, molecular weight

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Arabinoxylan (AX) is a major dietary fibre component found in a variety of cereals. Numerous health benefits of arabinoxylans have been reported to be associated with their solubility and molecular features. The current study reports the development of a functional bread using a combination of AX-enriched material (AEM) and optimal commercial endoxylanase. The total AX content of bread was increased to 8.2 g per 100 g available carbohydrates. The extractability of AX in breads with and without endoxylanase was determined. The results demonstrate that water-extractable AX (WE-AX) increased progressively through the bread making process. The application of endoxylanase also increased WE-AX content. The presence of 360 ppm of endoxylanase had positive effects on the bread characteristics in terms of bread volume and firmness by converting the water unextractable (WU)-AX to WE-AX. In addition, the molecular weight (Mw) distribution of the WE-AX of bread with and without endoxylanase was characterized by size-exclusion chromatography. The results show that as the portion of WE-AX increased, the amount of high Mw WE-AX (higher than 100 kDa) decreased, whereas the amount of low Mw WE-AX (lower than 100 kDa) increased from 33.2% to 44.2% through the baking process. The low Mw WE-AX further increased to 75.5% with the application of the optimal endoxylanase (360 ppm).


[1]  Anderson, W.J.; Baird, P.; Davis, H.R., Jr.; Ferreri, S.; Knudtson, M.; Koraym, A.; Waters, V.; Williams, L.C. Health benefits of dietary fiber. Nutr. Rev. 2009, 67, 188–205, doi:10.1111/j.1753-4887.2009.00189.x.
[2]  Yadav, P.M.; Johnston, B.D.; Hotchkiss, T.A., Jr.; Hicks, B.K. Corn fiber gum: A potential gum arabic replacer for beverage flavor emulsification. Food Hydrocol. 2007, 21, 1022–1030, doi:10.1016/j.foodhyd.2006.07.009.
[3]  Broekaert, W.F.; Courtin, C.M.; Verbeke, K.; van de Wiele, T.; Verstraete, W.; Delcour, J.A. Prebiotic and other health-related effects of cereal-derived arabinoxylans, arabinoxylan-oligosaccharides, and xylooligosaccharides. Crit. Rev. Food Sci. Nutr. 2011, 51, 178–194, doi:10.1080/10408390903044768.
[4]  Saeed, F.; Pasha, I.; Anjuma, M.F.; Sultan, T.M. Arabinoxylans and arabinogalactans: A comprehensive treatise. Crit. Rev. Food Sci. Nutr. 2011, 51, 467–476, doi:10.1080/10408391003681418.
[5]  Fran?ois, I.E; Lescroart, O.; Veraverbeke, W.S.; Marzorati, M.; Possemiers, S.; Evenepoel, P.; Hamer, H.; Houben, E.; Windey, K.; Welling, G.W.; et al. Effects of a wheat bran extract containing arabinoxylan oligosaccharides on gastrointestinal health parameters in healthy adult human volunteers: A double-blind, randomised, placebo-controlled, cross-over trial. Br. J. Nutr. 2012, 108, 2229–2242.
[6]  Toole, G.A.; le Gall, G.; Colquhoun, I.J.; Johnson, P.; Bedo, Z.; Saulnier, L.; Shewry, P.R.; Mills, E.N.C. Spectroscopic analysis of diversity of Arabinoxylan structures in endosperm cell walls of wheat cultivars (Triticum aestivum) in the HEALTHGRAIN diversity collection. J. Agric. Food Chem. 2011, 59, 7075–7082, doi:10.1021/jf201095m.
[7]  Damen, B.; Pollet, A.; Dornez, E.; Broekaert, W.F.; Haesendonck, I.V.; Trogh, I.; Arnaut, F.; Delcour, J.A.; Courtin, C.M. Xylanase-mediated in situ production of arabinoxylan oligosaccharides with prebiotic potential in whole meal breads and breads enriched with arabinoxylan rich material. Food Chem. 2012, 131, 111–118, doi:10.1016/j.foodchem.2011.08.043.
[8]  Lu, Z.X.; Walker, K.Z.; Muir, J.G.; Mascara, T.; O’Dea, K. Arabinoxylan fiber, a byproduct of wheat flour processing, reduces the postprandial glucose response in normoglycemic subjects. Am. J. Clin. Nutr. 2000, 71, 1123–1128.
[9]  Lu, Z.X.; Walker, K.Z.; Muir, J.G.; O’Dea, K. Arabinoxylan fibre improves metabolic control in people with Type II diabetes. Eur. J. Clin. Nutr. 2004, 58, 621–628, doi:10.1038/sj.ejcn.1601857.
[10]  Zhou, S.; Liu, X.; Guo, Y.; Wang, Q.; Peng, D.; Cao, L. Comparison of the immunological activities of arabinoxylans from wheat bran with alkali and xylanase-aided extraction. Carbohydr. Polym. 2010, 81, 784–789, doi:10.1016/j.carbpol.2010.03.040.
[11]  Cao, L.; Liu, X.Z.; Qian, T.X.; Sun, G.B.; Guo, Y.; Chang, F.J.; Zhou, S.M.; Sun, X.B. Antitumor and immunomodulatory activity of arabinoxylans: A major constituent of wheat bran. Int. J. Biol. Macromol. 2011, 48, 160–164, doi:10.1016/j.ijbiomac.2010.10.014.
[12]  Cloetens, L.; Swennen, K.; de Preter, V.; Broekaert, W.F.; Courtin, C.M.; Delcour, J.A.; Rutgeerts, P.; Verbeke, K. Effect of arabinoxylo-oligosaccharides on proximal gastrointestinal motility and digestion in healthy volunteers. e-SPEN 2008, 35, e220–e225.
[13]  Grootaert, C.; Delcour, J.A.; Courtin, C.M.; Broekaert, W.F.; Verstraete, W.; Wiele van de, T. Microbial metabolism and prebiotic potency of arabinoxylan oligosaccharides in the human intestine. Trends Food Sci. Technol. 2007, 18, 64–71, doi:10.1016/j.tifs.2006.08.004.
[14]  Grootaert, C.; van den Abbeele1, P.; Marzorati1, M.; Broekaert, W.F.; Courtin, M.C.; Delcour, J.A.; Verstraete1, W.; Wiele van de, T. Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS Microbiol. Ecol. 2009, 69, 231–242, doi:10.1111/j.1574-6941.2009.00712.x.
[15]  Maki, K.C.; Gibson, G.R.; Dickmann, R.S.; Kendall, C.W.C.; Chen, C.Y.O.; Costabile, A.; Comelli, E.M.; McKay, D.L.; Almeida, N.G.; Jenkins, D.; et al. Digestive and physiologic effects of a wheat bran extract, arabino-xylan-oligosaccharide, in breakfast cereal. Nutrition 2012, 28, 1115–1121, doi:10.1016/j.nut.2012.02.010.
[16]  U.S. Food and Drug Administration. Agency Response Letter GRAS Notice No. GRN 000343. CFSAN/Office of Food Additive Safety. Available online: IngredientsPackagingLabeling/GRAS/NoticeInventory/ucm235936.htm (access on 15 February 2013).
[17]  AACC Internationl. Approved Methods of Analysis, 11th Ed. Method 74-09.01. Measurement of Bread Firmness by Universal Testing Machine; AACC International: St. Paul, MN, USA, 1999.
[18]  Douglas, S.G. A rapid method for the determination of pentosans in wheat flour. Food Chem. 1980, 7, 139–145, doi:10.1016/0308-8146(81)90059-5.
[19]  Hollmann, J.; Lindhauer, M.G. Pilot-scale isolation of glucuronoarabinoxylans from wheat bran. Carbohydr. Polym. 2005, 59, 225–230, doi:10.1016/j.carbpol.2004.09.015.
[20]  Bataillon, M.; Mathaly, P.; Crdinali, A.P.N.; Duchiron, F. Extraction and purification ofarabinoxylan from destarched wheat bran in a pilot scale. Ind. Crop. Prod. 1998, 8, 37–43, doi:10.1016/S0926-6690(97)10002-4.
[21]  Courtin, C.M.; Delcour, J.A. Arabinoxylans and endoxylanases in wheat flour bread-making. J. Cereal Sci. 2002, 35, 225–243, doi:10.1006/jcrs.2001.0433.
[22]  Redgwell, R.J.; de Michieli, J.-H.; Fischer, M.; Reymond, S.; Nicolas, P.; Sievert, D. Xylanase induced changes to water- and alkaliextractable arabinoxylans in wheat flour: Their role in lowering batter viscosity. J. Cereal Sci. 2001, 33, 83–96, doi:10.1006/jcrs.2000.0345.
[23]  Skendi, A.; Biliaderis, C.G; Izydorczyk, M.S.; Zervou, M.; Zoumpoulakis, P. Structural variation and rheological properties of water-extractable arabinoxylans from six Greek wheat cultivars. Food Chem. 2011, 126, 526–536.
[24]  Courtin, C.M.; Delcour, J.A. Physicochemical and bread-making properties of low molecular weight wheat derived arabinoxylans. J. Agri. Food Chem. 1998, 46, 4066–4073, doi:10.1021/jf980339t.
[25]  Cleemput, G.; Booij, C.; Hessing, M.; Gruppen, H.; Delcour, J.A. Solubilisation and changes in molecular weight distribution of arabinoxylan and protein in wheat flours during bread-making, and the effects of endogenous arabinoxylan hydrolysing enzymes. J. Cereal Sci. 2000, 26, 55–66.
[26]  Goesaert, H.; Brijs, K.; Veraverbeke, W.S.; Courtin, C.M.; Gebruers, K.J.; Delcour, J.A. Wheat flour constituents: How they impact bread quality, and how to impact their functionality. Trends Food Sci. Technol. 2005, 16, 12–30.
[27]  Courtin, C.M.; Gelders, G.G.; Delcour, J.A. The use of two endoxylanases with different substrate selectivity provides insight into the functionality of arabinoxylans in wheat flour breadmaking. Cereal Chem. 2001, 78, 564–571, doi:10.1094/CCHEM.2001.78.5.564.
[28]  Henrissat, B.; Bairoch, A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 1993, 293, 781–788.
[29]  Reilly, P.J. Xylanases, Structure and Function. In Trends in the Biology of Fermentation for Fuels and Chemicals; Hollaender, A., Ed.; Plenum Press: New York, NY, USA, 1981; pp. 111–129.
[30]  Li, K.; Azadi, P.; Collins, R.; Tolan, J.; Kim, J.S.; Eriksson, K.L. Relationships between activities of xylanases and xylan structures. Enzyme Microb. Technol. 2000, 27, 89–94.
[31]  Vardakou, M.; Katapodis, P.; Samiotaki, M.; Kekos, D.; Panayotou, G.; Christakopoulos, P. Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan. Int. J. Biol. Macromol. 2003, 33, 129–134, doi:10.1016/S0141-8130(03)00077-1.
[32]  Pitkanen, L.; Tuomainen, P.; Virkki, L.; Tenkanen, M. Molecular characterization and solution properties of enzymatically tailored arabinoxylans. Int. J. Biol. Macromol. 2011, 49, 963–969, doi:10.1016/j.ijbiomac.2011.08.020.
[33]  Courtin, C.M.; Roelants, A.; Delcour, J.A. Fractionation-reconstitution experiments provide insight into the role of endoxylanases in bread-making. J. Agric. Food Chem. 1999, 47, 1870–1877, doi:10.1021/jf981178w.
[34]  Arnaut, F.; Willem, F.; Broekaert, W.F.; Courtin, C.; Delcour, J.A.; Georis, J.; van Haesendonck, I.P.H. Bread with Increased Arabinoxylo-Oligosaccharide Content. U.S. Patent WO2008087167 A, 24 July 2008.
[35]  Makaravicius, T.; Basinskiene, L.; Juodeikiene, G.; van Gool, M.P.; Schols, H.A. Production of oligosaccharides from extruded wheat and rye biomass using enzymatic treatment. Catal. Today 2012, 196, 16–25.
[36]  M?hlig, M.; Koebnick, C.; Weickert, M.O; Lueder, W.; Otto, B.; Steiniger, J.; Twilfert, M.; Meuser, F.; Pfeiffer, A.F.; Zunft, H.J. Arabinoxylan-enriched meal increases serum ghrelin levels in healthy humans. Horm. Metab. Res. 2005, 37, 303–308.
[37]  European Food Safety Authority. Scientific Opinion on the substantiation of health claims related to arabinoxylan produced from wheat endosperm and reduction of post-prandial glycaemic responses (ID 830) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). EFSA J. 2011, 9, 2205.
[38]  Hughes, S.A.; Shewry, P.R.; Li, L.; Gibson, G.R.; Sanz, M.L.; Rastall, R.A. In vitro fermentation by human fecal microflora of wheat arabinoxylans. J. Agric. Food Chem. 2007, 55, 4589–4595.
[39]  Vardakou, M.; Palop, C.N.; Christakopoulos, P.; Faulds, C.B.; Gasson, M.A.; Narbad, A. Evaluation of the prebiotic properties of wheat arabinoxylan fractions and induction of hydrolase activity in gut microflora. Int. J. Food Microbiol. 2008, 123, 166–170, doi:10.1016/j.ijfoodmicro.2007.11.007.


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