The aim of the present study evaluates the fat-soluble vitamins all- trans retinol (vitamin A), cholecalciferol (vitamin D 3) and α-tocopherol (vitamin E) content in the fresh edible tissue of Bulgarian fish species: marine—grey mullet ( Mugil cephalus) and bonito ( Sarda sarda), and freshwater—rainbow trout ( Oncorhynchus mykiss) and common carp ( Cyprinus carpio). The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction with n-hexane. All- trans retinol, cholecalciferol and α-tocopherol were analyzed simultaneously using RP-HPLC\UV\FL system with analytical column C18 ODS2 Hypersil？. The fat soluble vitamins content (μg per 100 g wet weight) in the fresh edible fish tissue of analyzed fishes are in the ranges: vitamin A from 2.7 ± 0.4 to 37.5 ± 3.4 μg/100 g ww; vitamin D 3 from 1.1 ± 0.1 to 11.4 ± 0.6 μg/100 g ww; vitamin E from 121.4 ± 9.6 to 1274.2 ± 44.1 μg/100 g ww. Three fat-soluble vitamins occur in higher amounts in rainbow trout and grey mullet species. According to recommended daily intake (RDI), they are a good source of cholecalciferol.
Szlinder-Richert, J.; Usydus, Z.; Malesa-Ciecwierz, M.; Polak-Juszczak, L.; Ruczynska, W. Marine and farmed fish on the Polish market: Comparison of the nutritive value and human exposure to PCDD/Fs and other contaminants. Chemosphere 2011, 85, 1725–1733, doi:10.1016/j.chemosphere.2011.09.019.
Krauss, R.M.; Deckelbaum, R.J.; Ernst, N.; Fisher, E.; Howard, B.V.; Knopp, R.H. Dietary guidelines for health American adults—A statement for health professionals from the Nutrition Committee. Circulation 1996, 94, 1795–1800, doi:10.1161/01.CIR.94.7.1795.
Ministry of Agriculture and Forestry; National Agency for Fisheries and Aquaculture. National Strategic Plan for Fisheries and Aquaculture, 2007–2013. Available online: http://ec.europa.eu/fisheries/cfp/eff/national_plans/list_of_national_strategic_plans/bulgaria_en.pdf (accessed on 11 October 2012).
Atanasov, A.; Nikolov, G.; Kiryakova, G.; Yordanova, L. Comparison of trout (Oncorhynchus mykiss) and carp (Cyprinus caprio) meat with other red and white meats(in Bulgarian). Trakia J. Sci. 2009, 7, pp. 200–202. Available online: http://tk.uni-sz.bg/files/E4_et_al.pdf (accessed on 11 October 2012).
Ahmadnia, A.; Sahari, M.A.; Barzegar, M.; Seyfabadi, S.J.; Abdollahi, M. Vitamins contents of some commercially important fish species from south Caspian Sea. Am. Eur. J. Sustain. Agric. 2008, 2, 285–293.
Stancheva, M.; Galunska, B.; Dobreva, D.A.; Merdzhanova, A. Retinol, alpha-tocopherol and fatty acid content in Bulgarian Black Sea fish species. Grasas Y Aceites 2012, 63, 152–157, doi:10.3989/gya.069611.
Ordinance No 23/19.07.2005 on the Physiological Feeding of Population. Available online: https://www.google.bg/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&ved=0CDcQFjAC&url=http%3A%2F%2Ffnap.government.bg%2FUserFiles%2FFile%2Fdoc%2FNAREDBA23%25202005%2520fiziologicnite_normi.doc&ei=zJq9UaLxKcmDONPXgKAE&usg=AFQjCNGLwv79EiYFN2JaEgM1pfS3lkJ0Jw&sig2=H-L2FDpDFgOBhXxlvqnpOw&bvm=bv.47883778,d.Yms (accessed on 9 November 2012).