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Agriculture  2013 

Risk Priority Number: A Measuring Instrument for Hygienic Management on Broiler Farms, Reflecting Their Campylobacter Status

DOI: 10.3390/agriculture3040700

Keywords: Campylobacter, control options, hygiene management, broiler farms

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Hygiene management is essential for rearing Campylobacter free broiler flocks. In this study, several hygiene factors (e.g., thinning, water supply, stable cloths, stable condition, stable environment, etc.) are categorized and aggregated in a developed risk priority number (RPN). This number is measuring the quality of hygiene management of a broiler farm with one single value (range: 801–4005 points), the higher the RPN, the better is the hygiene status. The distribution of the values is left skewed and none of the 53 examined Austrian broiler farms reached the maximum. Cecal samples ( n = 610) from broilers at the point of slaughter determined the Campylobacter status of the farms. Farms with a high RPN consistently produced more Campylobacter free batches than farms with a low RPN. Ranking of the broiler farms based on their RPN was significantly correlated with their microbiological results for Campylobacter detection (Spearman’s correlation coefficient = 0.646). The risk priority number is an easy tool for the assessment and measurement of the hygiene management system at a broiler farm. Besides the educational benefits of the RPN, benchmarking against the mean value or the maximum is possible.


[1]  European Food Safety Authority (EFSA). ECDC The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2011. EFSA J. 2013, 11, 3129, doi:10.2903/j.efsa.2013.3129.
[2]  Jelovcan, S.; Kornschober, C. Austrian Agency for Health and Food Safety: Graz, Austria, 2013.
[3]  Yu, R.K.; Usuki, S.; Ariga, T. Ganglioside molecular mimicry and its pathological roles in Guillain-Barre syndrome and related diseases. Infect. Immun. 2006, 74, 6517–6527, doi:10.1128/IAI.00967-06.
[4]  Hannu, T. Campylobacter-triggered reactive arthritis: A population-based study. Rheumatology 2002, 41, 312–318.
[5]  EFSA Panel on Biological Hazards (BIOHAZ). Scientific Opinion on Quantification of the risk posed by broiler meat to human campylobacteriosis in the EU. EFSA J. 2010, 8, doi:10.2903/j.efsa.2010.1437.
[6]  Scientific Committee of the Food Safety Authority of Ireland (FSAI). Food Safety Authority of Ireland: Dublin, Ireland, 2011.
[7]  Vellinga, A. The dioxin crisis as experiment to determine poultry-related campylobacter enteritis. Emerg. Infect. Dis. 2002, 8, 19–22, doi:10.3201/eid0801.010129.
[8]  Stern, N.J.; Hiett, K.L.; Alfredsson, G.A.; Kristinsson, K.G.; Reiersen, J.; Hardardottir, H.; Briem, H.; Gunnarsson, E.; Georgsson, F.; Lowman, R.; et al. Campylobacter spp. in Icelandic poultry operations and human disease. Epidemiol. Infect. 2003, 130, 23–32, doi:10.1017/S0950268802007914.
[9]  Mullner, P.; Jones, G.; Noble, A.; Spencer, S.E.F.; Hathaway, S.; French, N.P. Source attribution of food-borne zoonoses in New Zealand: A modified Hald model. Risk Anal. 2009, 29, 970–984, doi:10.1111/j.1539-6924.2009.01224.x.
[10]  Sheppard, S.K.; Jolley, K.A.; Maiden, M.C.J. A gene-by-gene approach to bacterial population genomics: Whole genome MLST of Campylobacter. Genes 2012, 3, 261–277, doi:10.3390/genes3020261.
[11]  Strachan, N.J.C.; Gormley, F.J.; Rotariu, O.; Ogden, I.D.; Miller, G.; Dunn, G.M.; Sheppard, S.K.; Dallas, J.F.; Reid, T.M.S.; Howie, H.; et al. Attribution of Campylobacter infections in northeast Scotland to specific sources by use of multilocus sequence typing. J. Infect. Dis. 2009, 199, 1205–1208, doi:10.1086/597417.
[12]  Newell, D.G.; Fearnley, C. Sources of Campylobacter colonization in broiler chickens. Appl. Environ. Microbiol. 2003, 69, 4343–4351, doi:10.1128/AEM.69.8.4343-4351.2003.
[13]  Sahin, O.; Kobalka, P.; Zhang, Q. Detection and survival of Campylobacter in chicken eggs. J. Appl. Microbiol. 2003, 95, 1070–1079, doi:10.1046/j.1365-2672.2003.02083.x.
[14]  EFSA. Scientific Opinion on Campylobacter in broiler meat production: Control options and performance objectives and/or targets at different stages of the food chain. EFSA J. 2011, 9, doi:10.2903/j.efsa.2011.2105.
[15]  Van Gerwe, T.J.W.M.; Bouma, A.; Jacobs-Reitsma, W.F.; van den Broek, J.; Klinkenberg, D.; Stegeman, J.A.; Heesterbeek, J.A.P. Quantifying transmission of Campylobacter spp. among broilers. Appl. Environ. Microbiol. 2005, 71, 5765–5770, doi:10.1128/AEM.71.10.5765-5770.2005.
[16]  P?lzler, T.; Wagner, M.; Slaghuis, J.; Schleicher, C.; K?fer, J. Rapid monitoring of Campylobacter in high-shedding flocks for targeted disease control. J. Food Prot. 2012, 75, 1835–1838, doi:10.4315/0362-028X.JFP-12-027.
[17]  Bouwknegt, M.; van de Giessen, A.W.; Dam-Deisz, W.D.C.; Havelaar, A.H.; Nagelkerke, N.J.D.; Henken, A.M. Risk factors for the presence of Campylobacter spp. in Dutch broiler flocks. Prev. Vet. Med. 2004, 62, 35–49, doi:10.1016/j.prevetmed.2003.09.003.
[18]  Hansson, I.; Engvall, E.O.; V?gsholm, I.; Nyman, A. Risk factors associated with the presence of Campylobacter-positive broiler flocks in Sweden. Prev. Vet. Med. 2010, 96, 114–121, doi:10.1016/j.prevetmed.2010.05.007.
[19]  Humphrey, T.; Paulsen, P.; Pfeifer, A.; Smulders, F.J.M. Campylobacter as the main zoonotic pathogen in poultry and strategies for its control. Vet. Med. Austria 2012, 99, 330–336.
[20]  Newell, D.G.; Elvers, K.T.; Dopfer, D.; Hansson, I.; Jones, P.; James, S.; Gittins, J.; Stern, N.J.; Davies, R.; Connerton, I.; et al. Biosecurity-based interventions and strategies to reduce Campylobacter spp. on poultry farms. Appl. Environ. Microbiol. 2011, 77, 8605–8614, doi:10.1128/AEM.01090-10.
[21]  Klein, G. Interventionsma?nahmen gegen Campylobacter in der Prim?rproduktion und in der Geflügelfleischgewinnung. Archiv für Lebensmittelhygiene 2010, 61, 108–111.
[22]  Nather, G.; Alter, T.; Martin, A.; Ellerbroek, L. Analysis of risk factors for Campylobacter species infection in broiler flocks. Poult. Sci. 2009, 88, 1299–1305, doi:10.3382/ps.2008-00389.
[23]  Berndtson, E.; Danielsson-Tham, M.-L.; Engvall, A. Campylobacter incidence on a chicken farm and the spread of Campylobacter during the slaughter process. Int. J. Food Microbiol. 1996, 32, 35–47, doi:10.1016/0168-1605(96)01102-6.
[24]  Amtliche Veterin?rnachrichten—Nr. 06/2013 vom 19. Juli 2013, GZ. 74.200/0024-II/B/10/2013; Ministry of Health: Vienna, Austria, 2008.
[25]  International Standard Analysis Techniques for System Reliability—Procedure for Failure Mode and Effects Analysis (FMEA). IEC 60812:2006; International Electrotechnical Commission: Geneva, Switzerland, 2006.
[26]  Aldridge, J.R.; Dole, B.G. Managing Quality, 4th ed.; Dole, B.G., Ed.; Blackwell Pub.: Malden, MA, USA, 2003; pp. 389–405.
[27]  EFSA. Scientific report of EFSA: Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008 Part A: Campylobacter and Salmonella prevalence estimates. EFSA J. 2010, 8, 1503, doi:10.2903/j.efsa.2010.1503.
[28]  Jorgensen, F.; Ellis-Iversen, J.; Rushton, S.; Bull, S.A.; Harris, S.A.; Bryan, S.J.; Gonzalez, A.; Humphrey, T.J. Influence of season and geography on Campylobacter jejuni and C. coli subtypes in housed broiler flocks reared in Great Britain. Appl. Environ. Microbiol. 2011, 77, 3741–3748.
[29]  Matt, M.; Weyermair, K.; Pless, P. Statistical Analysis of Risk Factors for Campylobacter Colonization at the Farm Level. In Proceedings of the XVth International Congress in Animal Hygiene (ISAH) Congress, Vienna, Austria, 3–7 July 2011; pp. 765–767.
[30]  Ellis-Iversen, J.; Ridley, A.; Morris, V.; Sowa, A.; Harris, J.; Atterbury, R.; Sparks, N.; Allen, V. Persistent environmental reservoirs on farms as risk factors for Campylobacter in commercial poultry. Epidemiol. Infect. 2012, 140, 916–924.
[31]  Hald, B.; Skovg?rd, H.; Bang, D.D.; Pedersen, K.; Dybdahl, J.; Jespersen, J.B.; Madsen, M. Flies and Campylobacter infection of broiler flocks. Emerg. Infect. Dis. 2004, 10, 1490–1492.
[32]  Sommer, H.M.; Heuer, O.E.; S?rensen, A.I.V.; Madsen, M. Analysis of factors important for the occurrence of Campylobacter in Danish broiler flocks. Prev. Vet. Med. 2013, 111, 100–111.
[33]  Verordnung der Bundesministerin für Gesundheit, Familie und Jugend über Gesundheitskontrollen und Hygienema?nahmen in Geflügel-Betrieben; Ministry of Health: Vienna, Austria, 2007.
[34]  Hald, B.; Sommer, H.M.; Skovg?rd, H. Use of fly screens to reduce Campylobacter spp. introduction in broiler houses. Emerg. Infect. Dis. 2007, 13, 1951–1953.
[35]  Hue, O.; Le Bouquin, S.; Laisney, M.-J.; Allain, V.; Lalande, F.; Petetin, I.; Rouxel, S.; Quesne, S.; Gloaguen, P.-Y.; Picherot, M.; et al. Prevalence of and risk factors for Campylobacter spp. contamination of broiler chicken carcasses at the slaughterhouse. Food Microbiol. 2010, 27, 992–999.
[36]  Ridley, A.M.; Morris, V.K.; Cawthraw, S.A.; Ellis-Iversen, J.; Harris, J.A.; Kennedy, E.M.; Newell, D.G.; Allen, V.M. Longitudinal molecular epidemiological study of thermophilic Campylobacters on one conventional broiler chicken farm. Appl. Environ. Microbiol. 2011, 77, 98–107.
[37]  Berrang, M.E.; Northcutt, J.K.; Cason, J.A. Recovery of Campylobacter from broiler feces during extended storage of transport cages. Poult. Sci. 2004, 83, 1213–1217.
[38]  Ridley, A.; Morris, V.; Gittins, J.; Cawthraw, S.; Harris, J.; Edge, S.; Allen, V. Potential sources of Campylobacter infection on chicken farms: Contamination and control of broiler-harvesting equipment, vehicles and personnel: Sources of Campylobacter associated with flock thinning. J. Appl. Microbiol. 2011, 111, 233–244.
[39]  Xiao, N.; Huang, H.-Z.; Li, Y.; He, L.; Jin, T. Multiple failure modes analysis and weighted risk priority number evaluation in FMEA. Eng. Fail. Anal. 2011, 18, 1162–1170.
[40]  Habib, I.; Berkvens, D.; de Zutter, L.; Dierick, K.; Van Huffel, X.; Speybroeck, N.; Geeraerd, A.H.; Uyttendaele, M. Campylobacter contamination in broiler carcasses and correlation with slaughterhouses operational hygiene inspection. Food Microbiol. 2012, 29, 105–112.
[41]  Stern, N.J.; Robach, M.C. Non-destructive sampling of live broiler for Campylobacter. J. Appl. Poult. Res. 1995, 4, 182–185.
[42]  . ISO 10272-1:2006; International Organization of Standardization: Geneva, Switzerland, 2006.
[43]  Pless, P.; Matt, M.; Wagner, P. Evaluation of Risk Factors Associated with Campylobacter spp. in Broiler Flocks. In Proceedings of the XVth International Congress in Animal Hygiene (ISAH) Congress, Vienna, Austria, 3–7 July 2011; pp. 185–188.


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