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Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

DOI: 10.1155/2012/157643

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Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30?g/L, Soybean cake flour: 23.53?g/L, KH2PO4: 4.90?g/L, and CoCl2·6H2O: 0.95?g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8?IU/mL. 1. Introduction The food and agricultural industries produce large volumes of wastes annually worldwide, causing serious disposal problems. This is more in countries where the economy is largely based on agriculture and farming practice is very intensive. Currently, these agrowastes are either allowed to decay naturally on the fields or are burnt. However, these wastes are rich in sugars due to their organic nature. They are easily assimilated by microorganisms and hence serve as source of potential substrates in the production of industrially relevant compounds through microbial conversion. In addition, the reutilization of biological wastes is of great interest since, due to legislation and environmental reasons, the industry is increasingly being forced to find an alternative use for its residual matter [1]. One of the agrowastes currently causing pollution problems is the peels of the mango (Mangifera indica L.) fruit. Mango is one of the most important fruits marketed in the world with a global production exceeding 26 million tons in 2004 [2]. It is cultivated or grown naturally in over 90 countries worldwide (mainly tropical and subtropical regions) and is known to be the second largest produced tropical fruit crop in the world [3]. The edible tissue makes up 33–85% of the fresh fruit, while the peel and the kernel amount to 7–24% and 9–40%, respectively [4]. In fact, mango peel as a byproduct of mango processing industry could be a rich source of bioactive compounds and enzymes such as protease, peroxidase, polyphenol oxidase, carotenoids, and vitamins C and E [5]. While the utilization of mango kernels as a source of fat, natural antioxidants, starch, flour, and feed has extensively been investigated [6, 7], studies on peels are


[1]  S. Rodríguez Couto, “Exploitation of biological wastes for the production of value-added products uncler solid-state fermentation conditions,” Biotechnology Journal, vol. 3, no. 7, pp. 859–870, 2008.
[2]  FAOSTAT, “FAO statistics, food and agriculture organization of the United Nations,” Rome, Italy, 2004,
[3]  J. K. Joseph and J. Abolaji, “Effects of replacing maize with graded levels of cooked Nigerian mango-seed kernels (Mangifera indica) on the performance, carcass yield and meat quality of broiler chickens,” Bioresource Technology, vol. 61, no. 1, pp. 99–102, 1997.
[4]  J. S. B. Wu, H. Chen, and T. Fang, “Mango juice,” in Fruit Juice Processing Technology Auburndale, S. Nagy, C. S. Chen, and P. E. Shaw, Eds., pp. 620–655, Agscience, Auburndale, Fa, USA, 1993.
[5]  C. M. Ajila, S. G. Bhat, and U. J. S. Prasada Rao, “Valuable components of raw and ripe peels from two Indian mango varieties,” Food Chemistry, vol. 102, no. 4, pp. 1006–1011, 2007.
[6]  S. S. Arogba, “Quality characteristics of a model biscuit containing processed mango (Mangifera indica) kernel flour,” International Journal of Food Properties, vol. 5, no. 2, pp. 249–260, 2002.
[7]  M. Kaur, N. Singh, K. S. Sandhu, and H. S. Guraya, “Physicochemical, morphological, thermal and rheological properties of starches separated from kernels of some Indian mango cultivars (Mangifera indica L.),” Food Chemistry, vol. 85, no. 1, pp. 131–140, 2004.
[8]  K. Madhukara, K. Nand, N. R. Raju, and H. R. Srilatha, “Ensilage of mangopeel for methane generation,” Process Biochemistry, vol. 28, no. 2, pp. 119–123, 1993.
[9]  M. Mahadevaswamy and L. V. Venkataraman, “Integrated utilization of fruit-processing wastes for biogas and fish production,” Biological Wastes, vol. 32, no. 4, pp. 243–251, 1990.
[10]  J. A. Larrauri, P. Rupérez, and F. Saura-Calixto, “Mango peel fibres with antioxidant activity,” European Food Research and Technology, vol. 205, no. 1, pp. 39–42, 1997.
[11]  N. Berardini, R. Fezer, J. Conrad, U. Beifuss, R. Carl, and A. Schieber, “Screening of mango (Mangifera indica L.) cultivars for their contents of flavonol O- and xanthone C-glycosides, anthocyanins, and pectin,” Journal of Agricultural and Food Chemistry, vol. 53, no. 5, pp. 1563–1570, 2005.
[12]  R. Pedroza-Islas and E. Aguilar-Esperanza, “Obtaining pectins from solid wastes derived from mango (Mangifera indica) processing,” AIChE Symposium Series, vol. 300, pp. 36–41, 1994.
[13]  D. K. Tandon and N. Garg, “Mango waste: a potential source of pectin, fiber, and starch,” Indian Journal of Environmental Protection, vol. 19, pp. 924–927, 1999.
[14]  N. Berardini, R. Carle, and A. Schieber, “Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. “Tommy Atkins”) peels, pulp and kernels by high-performance liquid chromatography/electrospray ionization mass spectrometry,” Rapid Communications in Mass Spectrometry, vol. 18, no. 19, pp. 2208–2216, 2004.
[15]  S. B. Riswanali, P. Saravanan, R. Muthuvelayudham, and T. Viruthagiri, “Optimization of nutrient medium for cellulase and hemicellulase productions from rice straw: a statistical approach,” International Journal of Chemical and Analytical Science, vol. 3, no. 4, pp. 1364–1370, 2012.
[16]  S. Bulut, M. Elibol, and D. Ozer, “Effect of different carbon sources on L(+) -lactic acid production by Rhizopus oryzae,” Biochemical Engineering Journal, vol. 21, no. 1, pp. 33–37, 2004.
[17]  R. Muthuvelayudham and T. Viruthagiri, “Application of central composite design based response surface methodology in parameter optimization and on cellulase production using agricultural waste,” International Journal of Chemical and Biological Engineering, vol. 3, no. 2, pp. 97–104, 2010.
[18]  T. K. Ghose, “Measurement of cellulase activities,” Pure and Applied Chemistry, vol. 59, no. 2, pp. 257–268, 1987.


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