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PLOS ONE  2013 

Cotton Fiber Cell Walls of Gossypium hirsutum and Gossypium barbadense Have Differences Related to Loosely-Bound Xyloglucan

DOI: 10.1371/journal.pone.0056315

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Cotton fiber is an important natural textile fiber due to its exceptional length and thickness. These properties arise largely through primary and secondary cell wall synthesis. The cotton fiber of commerce is a cellulosic secondary wall surrounded by a thin cuticulated primary wall, but there were only sparse details available about the polysaccharides in the fiber cell wall of any cotton species. In addition, Gossypium hirsutum (Gh) fiber was known to have an adhesive cotton fiber middle lamella (CFML) that joins adjacent fibers into tissue-like bundles, but it was unknown whether a CFML existed in other commercially important cotton fibers. We compared the cell wall chemistry over the time course of fiber development in Gh and Gossypium barbadense (Gb), the two most important commercial cotton species, when plants were grown in parallel in a highly controlled greenhouse. Under these growing conditions, the rate of early fiber elongation and the time of onset of secondary wall deposition were similar in fibers of the two species, but as expected the Gb fiber had a prolonged elongation period and developed higher quality compared to Gh fiber. The Gb fibers had a CFML, but it was not directly required for fiber elongation because Gb fiber continued to elongate rapidly after CFML hydrolysis. For both species, fiber at seven ages was extracted with four increasingly strong solvents, followed by analysis of cell wall matrix polysaccharide epitopes using antibody-based Glycome Profiling. Together with immunohistochemistry of fiber cross-sections, the data show that the CFML of Gb fiber contained lower levels of xyloglucan compared to Gh fiber. Xyloglucan endo-hydrolase activity was also higher in Gb fiber. In general, the data provide a rich picture of the similarities and differences in the cell wall structure of the two most important commercial cotton species.


[1]  Meinert MC, Delmer DP (1977) Changes in biochemical composition of the cell wall of the cotton fiber during development. Plant Physiol 59: 1088–1097.
[2]  Kim HJ, Triplett BA (2001) Cotton fiber growth in planta and in vitro. Models for plant cell elongation and cell wall biogenesis. Plant Physiol 127: 1361–1366.
[3]  Singh B, Avci U, Eichler Inwood SE, Grimson MJ, Landgraf J, et al. (2009) A specialized outer layer of the primary cell wall joins elongating cotton fibers into tissue-like bundles. Plant Physiol 150: 684–699.
[4]  Haigler CH, Betancur L, Stiff MR, Tuttle JR (2012) Cotton fiber: a powerful single-cell model for cell wall and cellulose research. Front Plant Sci 3: Article 104. Available: Accessed 2012 Aug 3.
[5]  Hovav R, Chaudhary B, Udall JA, Flagel L, Wendel JF (2008) Parallel domestication, convergent evolution and duplicated gene recruitment in allopolyploid cotton. Genetics 179: 1725–1733.
[6]  Wendel JF, Brubaker C, Alvarez I, Cronn R, Stewart JMcD (2009) Evolution and natural history of the cotton genus, In: Paterson AH, editor. Genetics and genomics of cotton, plant genetics and genomics: crops and models 3. New York: Springer Science+Business Media LLC: 3–22.
[7]  Hawkins RS, Serviss GH (1930) Development of cotton fibers in the pima and acala varieties. J Agric Res 40: 1017–1029.
[8]  Schubert AM, Benedict CR, Gates CE, Kohel RJ (1976) Growth and development of the lint fibers of Pima S-4 cotton. Crop Sci 16: 539–543.
[9]  Ruan YL, Xu SM, White R, Furbank RT (2004) Genotypic and developmental evidence for the role of plasmodesmatal regulation in cotton fiber elongation mediated by callose turnover. Plant Physiol 136: 4104–4113.
[10]  Beasley CA (1979) Cellulose content in fibers of cottons which differ in their lint lengths and extent of fuzz. Physiol Plant 45: 77–82.
[11]  Alabady MS, Youn E, Wilkins TA (2008) Double feature selection and cluster analysis in mining of microarray data from cotton. BMC Genomics 9: 295. Available: Accessed 2012 Jun 12.
[12]  Al-Ghazi Y, Bourot S, Arioli T, Dennis ES, Llewellyn DJ (2009) Transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality. Plant Cell Physiol 50: 1364–1381.
[13]  Claverie M, Souquet M, Jean J, Forestier-Chiron N, Lepitre V, et al. (2011) cDNA-AFLP-based genetical genomics in cotton fibers. Theor Appl Genet 124: 665–683.
[14]  Chen X, Guo W, Liu G, Zhang Y, Song X, et al. (2012) Molecular mechanisms for fiber differential development between G. barbadense and G. hirsutum revealed by genetical genomics. PLoS One 7(1), e30056. Available: Accessed 2012 Mar 30.
[15]  Wang L, Li XR, Lian H, Ni D-A, He Y-K, et al. (2010) Evidence that high activity of vacuolar invertase is required for cotton fiber and Arabidopsis root elongation through osmotic dependent and independent pathways, respectively. Plant Physiol 154: 744–756.
[16]  Li X-R, Wang L, Ruan Y-L (2010) Developmental and molecular physiological evidence for the role of phosphoenolpyruvate carboxylase in rapid cotton fibre elongation. J Expt Bot 61: 287–295.
[17]  O’Neill MA, York WS (2003) The composition and structure of primary cell walls. In: Rose JKC, editor. The plant cell wall, Annu Plant Rev (Volume 8). Boca Raton, FL: CRC Press. 1–54.
[18]  Mohnen D (2008) Pectin structure and synthesis. Curr. Opin. Plant Biol. 11, 266–277.
[19]  Lee KJD, Marcus SE, Knox JP (2011) Cell wall biology: perspectives from cell wall imaging. Mol Plant 4: 212–219.
[20]  Pattathil S, Avci U, Miller JS, Hahn MG (2012) Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. In: Himmel M, editor. Biomass conversion: methods and protocols, Methods Mol Biol (Volume 908). New York: Humana Press, 61–72.
[21]  Pattathil S, Avci U, Baldwin D, Alton GS, McGill JA, et al. (2010) A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol 153: 514–525.
[22]  Haigler CH, Singh B, Wang G, Zhang D (2009) Genomics of cotton fiber secondary wall deposition and cellulose biogenesis. In: New York: Springer Science+Business Media Paterson AH, editor. Genetics and genomics of cotton, plant genetics and genomics: crops and models 3. LLC: 385–417.
[23]  Zhu X, Pattathil S, Mazumder K, Brehm A, Hahn MG, et al. (2010) Virus-induced gene silencing offers a functional genomics platform for studying plant cell wall formation. Mol Plant 3(5): 818–833.
[24]  DeMartini JD, Pattathil S, Avci U, Szekalski K, Mazumder K, et al. (2011) Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ Sci 4(10): 4332–4339.
[25]  Meikle PJ, Bonig I, Hoogenraad NJ, Clarke AE, Stone BA (1991) The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody. Planta 185: 1–8.
[26]  Fry SC (1988) The growing plant cell wall: chemical and metabolic analysis. New York: John Wiley and Sons Inc. 333 p.
[27]  DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356.
[28]  Masuko T, Minami A, Iwasaki N, Majima T, Nishimura S, et al. (2005) Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal Biochem 339: 69–72.
[29]  Willats WGT, Limberg G, Buchholt HC, van Alebeek G-J, Benen J, et al. (2000) Analysis of pectin epitopes recognized by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr Res 327: 309–320.
[30]  Avci U, Pattathil S, Hahn MG (2012) Immunological approaches to plant cell wall and biomass characterization: Immunolocalization of glycan epitopes. In Biomass Conversion: Methods and Protocols. Methods Mol. Biol., Vol. 908, Himmel, M., ed. (New York: Humana Press), 73–82.
[31]  Sulova Z, Lednicka M, Farkas V (1995) A colorimetric assay for xyloglucan-endotransglycosylase from germinating seeds. Anal Biochem 229: 80–85.
[32]  Betancur L, Singh B, Rapp RA, Wendel JF, Marks MD, et al. (2010) Phylogenetically distinct cellulose synthase genes support secondary wall thickening in arabidopsis shoot trichomes and cotton fiber. J Integr Plant Biol 52: 205–220.
[33]  Cosgrove DJ (2005). Growth of the plant cell wall. Nature Rev Mol Cell Biol 6, 850–861.
[34]  Hequet E, Wyatt B, Abidi N (2006) Creation of a set of reference material for cotton fiber maturity measurements. Text Res J 76: 576–586.
[35]  Salnikov V, Grimson MJ, Seagull RW, Haigler CH (2003) Localization of sucrose synthase and callose in freeze substituted, secondary wall stage, cotton fibers. Protoplasma 221, 175–184.
[36]  Puhlmann J, Bucheli E, Swain MJ, Dunning N, Albersheim P, et al. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1–2)-linked fucosyl-containing epitope. Plant Physiol 104: 699–710.
[37]  ?bro J, Hayashi T, Mikkelsen JD (2011) Enzymatic modification of plant cell wall polysaccharides. In: Ulvskov P, editor. Plant polysaccharides, biosynthesis and bioengineering, Annu Plant Rev (Volume 41). Chichester UK: John Wiley and Sons Ltd. 367–388.
[38]  Buchala AJ (1999) Noncellulosic carbohydrates in cotton fibers. In: Basra AS, editor. Cotton fibers-developmental biology, quality improvement, and textile processing. New York: Haworth Press Inc. 113–136.
[39]  Bowman DT, May OL, Calhoun DS (1997) Coefficients of parentage for 260 cotton cultivars released between 1970 and 1990. U.S. Dept of Agric Tech Bull Issue 1852, 80 p.
[40]  Beasley CA (1977) Temperature-dependent response to indolacetic acid is altered by NH4+ in cultured cotton ovules. Plant Physiol 59: 203–206.
[41]  Hayashi T, Delmer DP (1988) Xyloglucan in the cell walls of cotton fiber. Carbohydr Res 181: 273–277.
[42]  Buchala AJ, Meier H (1981) An arabinogalactan from the fibres of cotton (Gossypium arboreum L). Carbohydr Res 89: 137–143.
[43]  Maltby D, Carpita NC, Montezinos D, Kulow C, Delmer DP (1979) ?-1,3-Glucan in developing cotton fibers. Plant Physiol 63: 1158–1164.
[44]  Jacquet J-P, Buchala AJ, Meier H (1982) Changes in the non-structural carbohydrate content of cotton (Gossypium spp.) fibres at different stages of development. Planta 156: 481–486.
[45]  Doblin MS, Pettolino F, Bacic A (2010) Plant cell walls: the skeleton of the plant world. Funct Plant Biol 37: 357–381.
[46]  Fry SC (2011) Cell wall polysaccharide composition and covalent crosslinking. In: Ulvskov P, editor. Plant polysaccharides, biosynthesis and bioengineering, Annu Plant Rev (Volume 41). Chichester UK: John Wiley and Sons Ltd. 1–42.
[47]  Clausen MH, Willats WGT, Knox JP (2003) Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res 338: 1797–1800.
[48]  Cavalier DM, Lerouxel O, Neumetzler L, Yamauchi K, Reinecke A, et al. (2008) Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component. Plant Cell 20: 1519–1537.
[49]  Bowling AJ, Vaughn KC, Turley RB (2011) Polysaccharide and glycoprotein distribution in the epidermis of cotton ovules during early fiber initiation and growth. Protoplasma 248: 579–590.
[50]  Lee J, Burns TH, Light G, Sun Y, Fokar M, et al. (2010) Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation. Planta 232: 1191–1205.


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