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PLOS ONE  2013 

The Oxidant-Scavenging Abilities in the Oral Cavity May Be Regulated by a Collaboration among Antioxidants in Saliva, Microorganisms, Blood Cells and Polyphenols: A Chemiluminescence-Based Study

DOI: 10.1371/journal.pone.0063062

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Abstract:

Saliva has become a central research issue in oral physiology and pathology. Over the evolution, the oral cavity has evolved the antioxidants uric acid, ascorbate reduced glutathione, plasma-derived albumin and antioxidants polyphenols from nutrients that are delivered to the oral cavity. However, blood cells extravasated from injured capillaries in gingival pathologies, or following tooth brushing and use of tooth picks, may attenuate the toxic activities of H2O2 generated by oral streptococci and by oxidants generated by activated phagocytes. Employing a highly sensitive luminol-dependent chemiluminescence, the DPPH radical and XTT assays to quantify oxidant-scavenging abilities (OSA), we show that saliva can strongly decompose both oxygen and nitrogen species. However, lipophilic antioxidant polyphenols in plants, which are poorly soluble in water and therefore not fully available as effective antioxidants, can nevertheless be solubilized either by small amounts of ethanol, whole saliva or also by salivary albumin and mucin. Plant-derived polyphenols can also act in collaboration with whole saliva, human red blood cells, platelets, and also with catalase-positive microorganisms to decompose reactive oxygen species (ROS). Furthermore, polyphenols from nutrient can avidly adhere to mucosal surfaces, are retained there for long periods and may function as a “slow- release devises” capable of affecting the redox status in the oral cavity. The OSA of saliva is due to the sum result of low molecular weight antioxidants, albumin, polyphenols from nutrients, blood elements and microbial antioxidants. Taken together, saliva and its antioxidants are considered regulators of the redox status in the oral cavity under physiological and pathological conditions.

References

[1]  Fábián TK, Fejérdy P, Csermely P (2008) Chemical biology of saliva in health and disease; Begley TP, editor. New York: John Wiley & Sons.
[2]  Grisham MB, Ryan EM (1990) Cytotoxic properties of salivary oxidants. American Journal of Physiology-Cell Physiology 258: C115–C121.
[3]  Halliwell B, Gutteridge JMC (2007) Free radicals in biology and medicine: Oxford University Press.
[4]  Thomas EL, Aune TM (1978) Lactoperoxidase, peroxide, thiocyanate antimicrobial system: correlation of sulfhydryl oxidation with antimicrobial action. Infect Immun 20: 456–463.
[5]  Sies H (2007) Biological redox systems and oxidative stress. Cell Mol Life Sci 64: 2181–2188.
[6]  Gutteridge JM, Halliwell B (2010) Antioxidants: Molecules, medicines, and myths. Biochem Biophys Res Commun 393: 561–564.
[7]  Sculley DV, Langley-Evans SC (2002) Salivary antioxidants and periodontal disease status. Proc Nutr Soc 61: 137–143.
[8]  Nagler RM, Klein I, Zarzhevsky N, Drigues N, Reznick AZ (2002) Characterization of the differentiated antioxidant profile of human saliva. Free Radic Biol Med 32: 268–277.
[9]  Amerongen AV, Veerman EC (2002) Saliva–the defender of the oral cavity. Oral Dis 8: 12–22.
[10]  Liskmann S, Vihalemm T, Salum O, Zilmer K, Fischer K, et al. (2007) Characterization of the antioxidant profile of human saliva in peri-implant health and disease. Clin Oral Implants Res 18: 27–33.
[11]  Toth KM, Clifford DP, Berger EM, White CW, Repine JE (1984) Intact human erythrocytes prevent hydrogen peroxide-mediated damage to isolated perfused rat lungs and cultured bovine pulmonary artery endothelial cells. J Clin Invest 74: 292–295.
[12]  Richards RS, Roberts TK, McGregor NR, Dunstan RH, Butt HL (1998) The role of erythrocytes in the inactivation of free radicals. Med Hypotheses 50: 363–367.
[13]  Ginsburg I, Kohen R, Koren E (2011) Microbial and host cells acquire enhanced oxidant-scavenging abilities by binding polyphenols. Arch Biochem Biophys 506: 12–23.
[14]  Koren E, Kohen R, Ovadia H, Ginsburg I (2009) Bacteria coated by polyphenols acquire potent oxidant-scavenging capacities. Exp Biol Med (Maywood) 234: 940–951.
[15]  Koren E, Kohen R, Ginsburg I (2010) Polyphenols enhance total oxidant-scavenging capacities of human blood by binding to red blood cells. Exp Biol Med (Maywood) 235: 689–699.
[16]  Ginsburg I, Koren E, Shalish M, Kanner J, Kohen R (2012) Saliva increases the availability of lipophilic polyphenols as antioxidants and enhances their retention in the oral cavity. Arch Oral Biol 57: 1327–1334.
[17]  Ginsburg I, Kohen R, Koren E (2012) Saliva: a ‘solubilizer’ of lipophilic antioxidant polyphenols. Oral Dis doi:10.1111/odi.12038.
[18]  Ginsburg I, Sadovnik M, Sallon S, Milo-Goldzweig I, Mechoulam R, et al. (1999) PADMA-28, a traditional tibetan herbal preparation inhibits the respiratory burst in human neutrophils, the killing of epithelial cells by mixtures of oxidants and pro-inflammatory agonists and peroxidation of lipids. Inflammopharmacology 7: 47–62.
[19]  Koren E, Kohen R, Ginsburg I (2009) A cobalt-based tetrazolium salts reduction test to assay polyphenols. J Agric Food Chem 57: 7644–7650.
[20]  Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
[21]  Navazesh M (1993) Methods for Collecting Saliva. Annals of the New York Academy of Sciences 694: 72–77.
[22]  Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in enzymology 299: 152–178.
[23]  Ginsburg I, Sadovnic M, Oron M, Kohen R (2004) Novel chemiluminescence-inducing cocktails, part II: measurement of the anti-oxidant capacity of vitamins, thiols, body fluids, alcoholic beverages and edible oils. Inflammopharmacology 12: 305–320.
[24]  Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181: 1199–1200.
[25]  Atsumi T, Iwakura I, Kashiwagi Y, Fujisawa S, Ueha T (1999) Free radical scavenging activity in the nonenzymatic fraction of human saliva: a simple DPPH assay showing the effect of physical exercise. Antioxid Redox Signal 1: 537–546.
[26]  Gorelik S, Ligumsky M, Kohen R, Kanner J (2008) A novel function of red wine polyphenols in humans: prevention of absorption of cytotoxic lipid peroxidation products. FASEB J 22: 41–46.
[27]  Kusuda M, Hatano T, Yoshida T (2006) Water-soluble complexes formed by natural polyphenols and bovine serum albumin: evidence from gel electrophoresis. Biosci Biotechnol Biochem 70: 152–160.
[28]  Kratz F (2008) Albumin as a drug carrier: design of prodrugs, drug conjugates and nanoparticles. J Control Release 132: 171–183.
[29]  Mueller S, Riedel HD, Stremmel W (1997) Direct evidence for catalase as the predominant H2O2 -removing enzyme in human erythrocytes. Blood 90: 4973–4978.
[30]  Lee MJ, Lambert JD, Prabhu S, Meng X, Lu H, et al. (2004) Delivery of tea polyphenols to the oral cavity by green tea leaves and black tea extract. Cancer Epidemiol Biomarkers Prev 13: 132–137.
[31]  Mathes SH, Wohlwend L, Uebersax L, von Mentlen R, Thoma DS, et al. (2010) A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnol Bioeng 107: 1029–1039.
[32]  Chapple IL, Brock G, Eftimiadi C, Matthews JB (2002) Glutathione in gingival crevicular fluid and its relation to local antioxidant capacity in periodontal health and disease. Mol Pathol 55: 367–373.
[33]  Frankel EN, Kanner J, German JB, Parks E, Kinsella JE (1993) Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 341: 454–457.
[34]  Baxter NJ, Lilley TH, Haslam E, Williamson MP (1997) Multiple interactions between polyphenols and a salivary proline-rich protein repeat result in complexation and precipitation. Biochemistry 36: 5566–5577.
[35]  Mehansho H, Butler LG, Carlson DM (1987) Dietary tannins and salivary proline-rich proteins: interactions, induction, and defense mechanisms. Annu Rev Nutr 7: 423–440.
[36]  Lotito SB, Fraga CG (2000) Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma. Biol Res 33: 151–157.
[37]  Lambert JD, Elias RJ (2010) The antioxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention. Arch Biochem Biophys 501: 65–72.
[38]  Santangelo C, Vari R, Scazzocchio B, Di Benedetto R, Filesi C, et al. (2007) Polyphenols, intracellular signalling and inflammation. Ann Ist Super Sanita 43: 394–405.
[39]  Grossman S, Dovrat S, Bergman M (2011) Natural antioxidants: just free radical scavengers or much more? Trends in Cancer Research, Volume 7: 57–73.
[40]  Hua Y, Nakamura T, Keep RF, Wu J, Schallert T, et al. (2006) Long-term effects of experimental intracerebral hemorrhage: the role of iron. J Neurosurg 104: 305–312.
[41]  Hamilton-Miller JM (1995) Antimicrobial properties of tea (Camellia sinensis L.). Antimicrob Agents Chemother 39: 2375–2377.
[42]  Ofek I, Hasty DL, Sharon N (2003) Anti-adhesion therapy of bacterial diseases: prospects and problems. FEMS Immunol Med Microbiol 38: 181–191.
[43]  Sakanaka S, Aizawa M, Kim M, Yamamoto T (1996) Inhibitory effects of green tea polyphenols on growth and cellular adherence of an oral bacterium, Porphyromonas gingivalis. Biosci Biotechnol Biochem 60: 745–749.
[44]  Ginsburg I (1998) Could synergistic interactions among reactive oxygen species, proteinases, membrane-perforating enzymes, hydrolases, microbial hemolysins and cytokines be the main cause of tissue damage in infectious and inflammatory conditions? Med Hypotheses 51: 337–346.
[45]  Ginsburg I, Kohen R (1995) Cell damage in inflammatory and infectious sites might involve a coordinated “cross-talk” among oxidants, microbial haemolysins and ampiphiles, cationic proteins, phospholipases, fatty acids, proteinases and cytokines (an overview). Free Radic Res 22: 489–517.
[46]  Gorelik S, Kanner J, Kohen R (2012) Additional ways to diminish the deleterious effects of red meat. Arch Intern Med 172: 1424–1425.

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