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PLOS ONE  2013 

Genetic and Antigenic Characterization of Enterovirus 71 in Ho Chi Minh City, Vietnam, 2011

DOI: 10.1371/journal.pone.0069895

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Enterovirus 71 (EV71) frequently causes fatal infections in young children in Asia. In 2011, EV71 epidemics occurred in southern Vietnam. We conducted genetic and antigenic analysis of the EV71 isolates and found that 94% of them were genotype C4a related to two lineages circulating in China and 6% were genotype C5 which have circulated in Vietnam since 2003. Antigenic variants were not detected. EV71 vaccines are being developed. Longitudinal enterovirus surveillance data are critical to formulate vaccination policy in Vietnam.


[1]  van der Sanden S, Koopmans M, Uslu G, van der Avoort H (2009) Epidemiology of enterovirus 71 in the Netherlands, 1963 to 2008. J Clin Microbiol 47: 2826-2833. doi:10.1128/JCM.00507-09. PubMed: 19625480.
[2]  Tee KK, Lam TT, Chan YF, Bible JM, Kamarulzaman A et al. (2010) Evolutionary genetics of human enterovirus 71: origin, population dynamics, natural selection, and seasonal periodicity of the VP1 gene. J Virol 84: 3339-3350. doi:10.1128/JVI.01019-09. PubMed: 20089660.
[3]  Lee MS, Tseng FC, Wang JR, Chi CY, Chong P et al. (2012) Challenges to licensure of enterovirus 71 vaccines. PLOS Negl Trop Dis 6: e1737. doi:10.1371/journal.pntd.0001737. PubMed: 22953003.
[4]  Rao CD, Yergolkar P, Shankarappa KS (2012) Antigenic diversity of enteroviruses associated with nonpolio acute flaccid paralysis, India, 2007-2009. Emerg Infect Dis 18: 1833-1840. doi:10.3201/eid1811.111457. PubMed: 23092622.
[5]  Tu PV, Thao NT, Perera D, Huu TK, Tien NT et al. (2007) Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005. Emerg Infect Dis 13: 1733-1741. doi:10.3201/eid1311.070632. PubMed: 18217559.
[6]  World Health Organization (2011) A Guide to Clinical Management and Public Health Response for Hand, Foot and Mouth Disease (HFMD). Geneva: WHO.
[7]  Khanh TH, Sabanathan S, Thanh TT, Thoa le PK, Thuong TC et al. (2012) Enterovirus 71-associated Hand, Foot, and Mouth Disease, Southern Vietnam, 2011. Emerg Infect Dis 18.
[8]  Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596-1599. doi:10.1093/molbev/msm092. PubMed: 17488738.
[9]  Huang SW, Hsu YW, Smith DJ, Kiang D, Tsai HP et al. (2009) Reemergence of enterovirus 71 in 2008 in taiwan: dynamics of genetic and antigenic evolution from 1998 to 2008. J Clin Microbiol 47: 3653-3662. doi:10.1128/JCM.00630-09. PubMed: 19776232.
[10]  Zhao K, Han X, Wang G, Hu W, Zhang W et al. (2011) Circulating coxsackievirus A16 identified as recombinant type A human enterovirus, China. Emerg Infect Dis 17: 1537-1540. PubMed: 21801645.
[11]  Wang X, Zhu C, Bao W, Zhao K, Niu J et al. (2012) Characterization of full-length enterovirus 71 strains from severe and mild disease patients in northeastern China. PLOS ONE 7: e32405. doi:10.1371/journal.pone.0032405. PubMed: 22479324.
[12]  Huang ML, Chiang PS, Chia MY, Luo ST, Chang LY et al. (2013) Cross-reactive Neutralizing Antibody Responses to Enterovirus 71 Infections in Young Children: implications for vaccine development. PLOS Negl Trop Dis 7: , 7: e2067. PubMed: 23459633.
[13]  Lee MS, Lin TY, Chiang PS, Li WC, Luo ST et al. (2010) An investigation of epidemic enterovirus 71 infection in Taiwan, 2008: clinical, virologic, and serologic features. Pediatr Infect Dis J 29: 1030-1034..
[14]  Lee MS, Chiang PS, Luo ST, Huang ML, Liou GY et al. (2012) Incidence rates of enterovirus 71 infections in young children during a nationwide epidemic in Taiwan, 2008-09. PLOS Negl Trop Dis 6: e1476. doi:10.1371/journal.pntd.0001476. PubMed: 22348156.
[15]  Zhang Y, Wang J, Guo W, Wang H, Zhu S et al. (2011) Emergence and transmission pathways of rapidly evolving evolutionary branch C4a strains of human enterovirus 71 in the Central Plain of China. PLOS ONE 6: e27895. doi:10.1371/journal.pone.0027895. PubMed: 22125635.
[16]  Huang YP, Lin TL, Kuo CY, Lin MW, Yao CY et al. (2008) The circulation of subgenogroups B5 and C5 of enterovirus 71 in Taiwan from 2006 to 2007. Virus Res 137: 206-212. doi:10.1016/j.virusres.2008.07.015. PubMed: 18706461.
[17]  McMinn PC (2012) Recent advances in the molecular epidemiology and control of human enterovirus 71 infection. Curr Opin Virol 2: 199-205. doi:10.1016/j.coviro.2012.02.009. PubMed: 22482716.
[18]  Tran CB, Nguyen HT, Phan HT, Tran NV, Wills B et al. (2011) The seroprevalence and seroincidence of enterovirus71 infection in infants and children in ho chi minh city, viet nam. PLOS ONE 6: e21116. doi:10.1371/journal.pone.0021116. PubMed: 21765891.
[19]  Chi CY, Khanh TH, Thoa LP, Tseng FC, Wang SM et al. (2013) Milrinone Therapy for Enterovirus 71-Induced Pulmonary Edema and/or Neurogenic Shock in Children: A Randomized Controlled Trial*. Crit Care Med 41: 1754-1760. doi:10.1097/CCM.0b013e31828a2a85. PubMed: 23685637.
[20]  Singh S, Chow VT, Phoon MC, Chan KP, Poh CL (2002) Direct detection of enterovirus 71 (EV71) in clinical specimens from a hand, foot, and mouth disease outbreak in Singapore by reverse transcription-PCR with universal enterovirus and EV71-specific primers. J Clin Microbiol 40: 2823-2827. doi:10.1128/JCM.40.8.2823-2827.2002. PubMed: 12149336.
[21]  Chiang PS, Huang ML, Luo ST, Lin TY, Tsao KC et al. (2012) Comparing molecular methods for early detection and serotyping of enteroviruses in throat swabs of pediatric patients. PLOS ONE 7: e48269. doi:10.1371/journal.pone.0048269. PubMed: 23133580.


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