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PLOS ONE  2013 

Prevalence of Herpes and Respiratory Viruses in Induced Sputum among Hospitalized Children with Non Typical Bacterial Community-Acquired Pneumonia

DOI: 10.1371/journal.pone.0079477

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Abstract:

Objective Few comprehensive studies have searched for viruses in infants and young children with community-acquired pneumonia (CAP) in China. The aim of this study was to investigate the roles of human herpes viruses (HHVs) and other respiratory viruses in CAP not caused by typical bacterial infection and to determine their prevalence and clinical significance. Methods Induced sputum (IS) samples were collected from 354 hospitalised patients (infants, n = 205; children, n = 149) with respiratory illness (CAP or non-CAP) admitted to Wenling Hospital of China. We tested for HHVs and respiratory viruses using PCR-based assays. The epidemiological profiles were also analysed. Results High rate of virus detection (more than 98%) and co-infection (more than 80%) were found among IS samples from 354 hospitalised infants and children with respiratory illness in this study. Of 273 CAP samples tested, CMV (91.6%), HHV-6 (50.9%), RSV (37.4%), EBV (35.5%), HBoV (28.2%), HHV-7 (18.3%) and rhinovirus (17.2%) were the most commonly detected viruses. Of 81 non- CAP samples tested, CMV (63%), RSV (49.4%), HHV-6 (42%), EBV (24.7%), HHV-7 (13.6%) and HBoV (8.6%) were the dominant viruses detected. The prevalence of several viral agents (rhinovirus, bocavirus, adenovirus and CMV) among IS samples of CAP were significantly higher than that of non-CAP control group. We also found the prevalence of RSV coinfection with HHVs was also higher among CAP group than that of non-CAP control. Conclusions With sensitive molecular detection techniques and IS samples, high rates of viral identification were achieved in infants and young children with respiratory illness in a rural area of China. The clinical significance of rhinovirus, bocavirus, adenovirus and HHV (especially CMV) infections should receive greater attention in future treatment and prevention studies of CAP in infants and children.

References

[1]  Mathers C, Bernard C, Moesgaard Iburg K, et al.. (2008) WHO. Global burden of disease in 2002: data sources, methods and results. Available: http://www.who.int/healthinfo/paper54.pd?f. Accessed 10 November 2008.
[2]  Ruuskanen O, Lahti E, Jennings LC, Murdoch DR (2011) Viral pneumonia. Lancet 377: 1264–1275.
[3]  McIntosh K (2002) Community-acquired pneumonia in children. N Engl J Med 346(6): 429–437.
[4]  Juvén T, Mertsola J, Waris M, Leinonen M, Meurman O, et al. (2000) Etiology of community-acquired pneumonia in 254 hospitalized children. Pediatr Infect Dis J 19: 293–298.
[5]  Cevey-Macherel M, Galetto-Lacour A, Gervaix A, Siegrist CA, Bille J, et al. (2009) Etiology of community-acquired pneumonia in hospitalized children based on WHO clinical guidelines. Eur J Pediatr 168: 1429–1436.
[6]  Brittain-Long R, Westin J, Olofsson S, Lindh M, Andersson LM (2011) Access to a polymerase chain reaction assay method targeting 13 respiratory viruses can reduce antibiotics: a randomised, controlled trial. BMC Med 9: 44.
[7]  Michelow IC, Olsen K, Lozano J, Rollins NK, Duffy LB, et al. (2004) Epidemiology and clinical characteristics of community-acquired pneumonia in hospitalized children. Pediatrics 113: 701–707.
[8]  Nascimento-Carvalho CM, Ribeiro CT, Cardoso MR, Barral A, Araújo-Neto CA, et al. (2008) The role of respiratory viral infections among children hospitalized for community-acquired pneumonia in a developing country. Pediatr Infect Dis J 27: 939–941.
[9]  Hamano-Hasegawa K, Morozumi M, Nakayama E, Chiba N, Murayama SY, et al. (2008) Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia. J Infect Chemother 14: 424–432.
[10]  O′Callaghan-Gordo C, Bassat Q, Morais L, Díez-Padrisa N, Machevo S, et al. (2011) Etiology and epidemiology of viral pneumonia among hospitalized children in rural Mozambique: a malaria endemic area with high prevalence of human immunodeficiency virus. Pediatr Infect Dis J 30: 39–44.
[11]  Berkley JA, Munywoki P, Ngama M, Kazungu S, Abwao J, et al. (2010) Viral etiology of severe pneumonia among Kenyan infants and children. JAMA 303: 2051–2057.
[12]  Tsolia MN, Psarras S, Bossios A, Audi H, Paldanius M, et al. (2004) Etiology of community-acquired pneumonia in hospitalized school-age children: evidence for high prevalence of viral infections. Clin Infect Dis 39: 681–686.
[13]  Templeton KE, Scheltinga SA, van den Eeden WC, Graffelman AW, van den Broek PJ, et al. (2005) Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction. Clin Infect Dis 41: 345–351.
[14]  Lahti E, Peltola V, Waris M, Virkki R, Rantakokko-Jalava K, et al. (2009) Induced sputum in the diagnosis of childhood community-acquired pneumonia. Thorax 64: 252–257.
[15]  Honkinen M, Lahti E, Sterback RO, Ruuskanen O, Waris M (2012) Viruses and bacteria in sputum samples of children with community-acquired pneumonia. CMI 18(3): 300–7.
[16]  Mermond S, Zurawski V, D′Ortenzio E, Driscoll AJ, DeLuca AN, et al. (2012) Lower respiratory infections among hospitalized children in New Caledonia: a pilot study for the Pneumonia Etiology Research for Child Health project. Clin Infect Dis 54 Suppl 2S180–S189.
[17]  Hall CB, Weinberg GA, Iwane MK, Blumkin AK, Edwards KM, et al. (2009) The burden of respiratory syncytial virus infection in young children. N Engl J Med 360(6): 588–598.
[18]  Peltola V, Waris M, Osterback R, Susi P, Ruuskanen O, et al. (2008) Rhinovirus transmission within families with children: incidence of symptomatic and asymptomatic infections. J Infect Dis 197: 382–389.
[19]  Solidoro P, Libertucci D, Delsedime L, Ruffini E, Bosco M, et al. (2008) Combined cytomegalovirus prophylaxis in lung transplantation: effects on acute rejection, lymphocytic bronchitis/bronchiolitis, and herpesvirus infections. Transplant Proc 40(6): 2013–2014.
[20]  Jaber S, Chanques G, Borry J, Souche B, Verdier R, et al. (2005) Cytomegalovirus infection in critically ill patients: associated factors and consequences. Chest 127(1): 233–241.
[21]  Costa C, Delsedime L, Solidoro P, Curtoni A, Bergallo M, et al. (2010) Herpesviruses detection by quantitative real-time polymerase chain reaction in bronchoalveolar lavage and transbronchial biopsy in lung transplant: viral infections and histopathological correlation. Transplant Proc 42(4): 1270–1274.
[22]  Levy JA (1997) Three new human herpesviruses (HHV6, 7, and 8). Lancet 349: 558–562.
[23]  Longtin J, Bastien M, Gilca R, Leblanc E, de Serres G, et al. (2008) Human bocavirus infections in hospitalized children and adults. Emerg Infect Dis 14: 217–221.
[24]  Fry AM, Lu X, Chittaganpitch M, Peret T, Fischer J, et al. (2007) Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis 195: 1038–1045.
[25]  Greenberg SB (2011) Update on rhinovirus and coronavirus infections. Semin Resoir Crit Care Med 32: 433–446.
[26]  Tanaka T, Kogawa K, Sasa H, Nonoyama S, Furuya K, et al. (2009) Rapid and simultaneous detection of 6 types of human herpes virus (herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpes virus 6A/B, and human herpes virus 7) by multiplex PCR assay. Biomedical Research 30(5): 279–285.
[27]  Yu X, Lu R, Wang Z, Zhu N, Wang W, et al. (2012) Etiology and clinical characterization of respiratory virus infections in adult patients attending an emergency department in Beijing. PLoS One 7(2): e32174.
[28]  Mackay IM, Jacob KC, Woolhouse D, Waller K, Syrmis MW, et al. (2003) Molecular assays for detection of human metapneumovirus. J Clin Microbiol 41: 100–105.
[29]  Manning A, Russell V, Eastick K, Leadbetter GH, Hallam N, et al. (2006) Epidemiological profile and clinical associations of human bocavirus and other human parvoviruses. J Infect Dis 194: 1283–1290.
[30]  Lu R, Yu X, Wang W, Duan X, Zhang L, et al. (2012) Characterization of human coronavirus etiology in Chinese adults with acute upper respiratory tract infection by real-time RT-PCR assays. Plops One 7(6): e38638.
[31]  Angeles Marcos M, Camps M, Pumarola T, Antonio Martinez J, Martinez E, et al. (2006) The role of viruses in the aetiology of community-acquired pneumonia in adults. Antivir Ther 11: 351–359.
[32]  Gambarino S, Mantovani S, Astegiano S, Libertucci D, Solidoro P, et al. (2009) Lower respiratory tract viral infections in hospitalized adult patients. Minerva Med 100(5): 349–355.
[33]  Wang W, Cavailler P, Ren P, Zhang J, Dong W, et al. (2010) Molecular monitoring of causative viruses in child acute respiratory infection in endemo-epidemic situations in Shanghai. J Clin Virol 49(3): 211–218.
[34]  Principi N, Bosis S, Esposito S (2010) Effects of coronavirus infections in children. Emerg Infect Dis 16: 183–188.
[35]  Paranhos-Baccalà G, Komurian-Pradel F, Richard N, Vernet G, Lina B, et al. (2008) Mixed respiratory virus infections. J Clin Virol 43(4): 407–410.
[36]  Peng D, Zhao D, Liu J, Wang X, Yang K, et al. (2009) Multipathogen infections in hospitalized children with acute respiratory infections. Virol J 6: 155–161.
[37]  Cilla G, O?ate E, Perez-Yarza EG, Montes M, Vicente D, et al. (2008) Viruses in community-acquired pneumonia in children aged less than 3 years old: high rate of viral coinfection. J Med Virol 80: 1843–1849.
[38]  Abughali N, Khiyami A, Birnkrant DJ, Kumar ML (2002) Severe respiratory syncytial virus pneumonia associated with primary Epstein-Barr virus infection. Pediatr Pulmonol 33: 395–398.
[39]  Coisel Y, Bousbia S, Forel JM, Hraiech S, Lascola B, et al. (2012) Cytomegalovirus and herpes simplex virus effect on the prognosis of mechanically ventilated patients suspected to have ventilator-associated pneumonia. PLoS One 7(12): e51340.
[40]  Grilli E, Galati V, Bordi L, Taglietti F, Petrosillo N (2012) Cytomegalovirus pneumonia in immunocompetent host: case report and literature review. J Clin Virol 55(4): 356–359.
[41]  Limaye AP, Boeckh M (2010) CMV in critically ill patients: pathogen or bystander? Rev Med Virol 20(6): 372–379.

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