全部 标题 作者
关键词 摘要

PLOS ONE  2014 

Disturbances in Pro-Oxidant-Antioxidant Balance after Passive Body Overheating and after Exercise in Elevated Ambient Temperatures in Athletes and Untrained Men

DOI: 10.1371/journal.pone.0085320

Full-Text   Cite this paper   Add to My Lib

Abstract:

The aim of the study was to investigate pro-oxidant-antioxidant balance in two series of examinations with two types of stressors (exogenous heat and the combined exogenous and endogenous heat) in trained and untrained men. The exogenous stressor was provided by Finnish sauna session, whereas the combined stressor was represented by the exercise in elevated ambient temperature. The men from the two groups performed the physical exercise on a cycle ergometer with the load of 53±2% maximal oxygen uptake at the temperature of 33±1°C and relative humidity of 70% until their rectal temperature rose by 1.2°C. After a month from completion of the exercise test the subjects participated in a sauna bathing session with the temperature of 96±2°C, and relative humidity of 16±5%. 15-minutes heating and 2-minute cool-down in a shower with the temperature of 20°C was repeated until rectal temperature rose by 1.2°C compared to the initial value. During both series of tests rectal temperature was measured at 5-minute intervals. Before both series of tests and after them body mass was measured and blood samples were taken for biochemical tests. Serum total protein, serum concentration of lipid peroxidation products and serum antioxidants were determined. The athletes were characterized by higher level of antioxidant status and lower concentration of lipid peroxidation products. Physical exercise at elevated ambient temperature caused lower changes in oxidative stress indices compared to sauna bathing. Sauna induced a shift in pro-oxidant-antioxidant balance towards oxidation, which was observed less intensively in the athletes compared to the untrained men. This leads to the conclusion that physical exercise increases tolerance to elevated ambient temperature and oxidative stress.

References

[1]  Powers SK, Jackson MJ (2008) Exercise-Induced Oxidative Stress: Cellular mechanisms and impact on muscle force production. PhysiolRev 88: 1243–1276.
[2]  Zembroń-?acny A, Szyszka K (2002) Zaburzenia równowagi prooksydacyjno-antyoksydacyjnej wywo?ane laboratoryjnym testem wios?larskim 2000 m. Med Sport 6 (2): 29–39.
[3]  Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, et al. (2000) Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. MedSci Sports Exerc 32(9): 1576–1581.
[4]  Hübner-Wo?niak E, Lutos?awska G, Panczenko-Kresowska B, Pos?nik J (2000) Spoczynkowe st??enie nadtlenków lipidowych w osoczu zredukowanego glutationu w krwinkach czerwonych u kobiet i m??czyzn trenuj?cych biatlon. Med Sport 4 (4): 257–264.
[5]  Goto C, Higashi Y, Kimura M, Noma K, Hara K, et al. (2003) Effect of different intensities of exercise on endothelium-dependent vasodilation in humans: role of endothelium-dependent nitric oxide and oxidative stress. Circulation 108(5): 530–535 doi:10.1161/01.CIR.0000080893.55729.28. PMID:12874192.
[6]  Knez WL, Jenkins DG, Coombes JS (2007) Oxidative stress in half and full Ironman triathletes. Med Sci Sports Exerc 39(2): 283–288 doi:10.1249/01.mss.0000246999.09718.0c. PMID: 17277592.
[7]  Zembroń-?acny A, Szyszka K, Hübner-Wo?niak E (1998) The pro-oxidant-antioxidant balance in the blood of middle – distance runners. Biol Sport 16: 51–59.
[8]  Matsuo M, Kaneko T (2000) The chemistry of reactive oxygen species and related free radicals. In: Free radicals in exercise and aging (Radak Z, Eds). Hum Kin. Leeds 1–34.
[9]  Sawka MN, Burke LM, Eichner ER, Maughan RJ, Montain SJ, et al.. (2007) American College of Sports Medicine position stand. Exercise and fluid replacement. Med SciSports Exerc39(2): 377–390. PMID:17277604.
[10]  Paik IY, Jeong MH, Jin HE, Kim YI, Suh AR, et al. (2009) Fluid replacement following dehydration reduces oxidative stress during recovery. BiochemBiophys Res Commun 383(1): 103–107 doi:10.1016/j.bbrc.2009.03.135.
[11]  Slaughter MH, Lohman TG, Boileau RA, Horswill CA, Stillman RJ, et al. (1988) Skinfold equations for estimation of body fatness in children and youth. Hum Biol 60(5): 709–723.
[12]  DuBois D, DuBois EF (1916) Clinical calorimetry: a formula to estimate the appropriate surface area if height and weight be known. Arch Internal Med 17: 863–871.
[13]  Nielsen B, Kubica R, Bonnesen A, Rasmussen IB, Stok?osa J, et al. (1981) Physical work capacity after dehydration and hyperthermia. Scand J Sports Sci 3(1): 2–10.
[14]  Kubica R, Tyka A, ?uchowicz A, Czuba?a M (1996) Human acclimation to work in warm and humid environments. J PhysiolPharmacol 47: 515–524.
[15]  Harrison MH (1985) Effects on thermal stress and exercise on blood volume in humans. Physiol Rev 65(1): 149–209.
[16]  Wo?niak-Grygiel E, Kochańska-Dziurowicz A, Stanjek-Cichoracka A (2007) Is it necessary to correct athlete? hormones concentrations for plasma volume changes? Med Sport 11(4): 17–21.
[17]  Kraemer RR, Brown BS (1984) Alterations in plasma-volume-corected blood components of marathon runners and concomitant relationship to performance. Eur J of ApplPhysiol 55: 579–584.
[18]  Kubica R (1979) Analiza wahań wydolnos?ci fizycznej, wywo?anych zmianami ciep?oty cia?a i modyfikacjami gospodarki wodno-elektrolitowej ustroju. ActaPhysiol Pol 30(19): 5–12.
[19]  Piwonka RW, Robinson S, Gay VJ, Greninger L (1965) Preacclimatization of men to heat by training. J ApplPhysiol 20: 379–384.
[20]  Gonzales–Alonzo J, Teller C, Andersen SL, Jensen FB, Hyldig T, et al. (1999) Influence of body temperature in the development of fatigue during prolonged exercise in the heat. J ApplPhysiol 86(3): 1032–1039.
[21]  Garrett AT, Goossens NG, Rehrer NJ (2009) Induction and decay of short-term heat acclimation. Eur J ApplPhysiol 107(6): 659–671.
[22]  Wright HE, Selkirk GA, McLellan TM (2010) HPA and SAS responses to increasing core temperature during uncompensableexertional heat stress in trained and untrained males. Eur J ApplPhysiol 108(5): 987–997.
[23]  Robergs R, Roberts S (2000) Fundamental Principles of Exercise for Fitness. Performance and Health. New York: McGraw-Hill Companies 134–152.
[24]  McBridge JM, Kraemer WJ (1999) Free radicals, exercise and antioxidants. J Strength Cond Res 13: 175–183.
[25]  Halliwell B (1994) Free radicals and antioxidants: a personal review. Nutr Rev 58: 253–265.
[26]  Clemens M, Waller MD (1988) Lipid peroxidation in erythrocytes. ChemPhys Lipids 45: 251–268.
[27]  Weiss ST (1998) Tissue destruction by neutrophils. N Engl Med 320: 365–375.
[28]  Vandenboom R (2004) The myofibryllar complex and fatique: a review. Can J ApplPhysiol 29: 330–356.
[29]  Vincent HK, Powers SK, Demirel HA, Coombes JS, Naito H (1999) Exercise training protects against contraction induced lipid peroxidation in the diaphragm. Eur J ApplPhysiolOccupPhysiol79: 268–273.
[30]  Vincent HK, Powers SK, Stewart DJ, Demirel HA, Shanely RA, et al. (2000) Short-term exercise training improves diaphragm antioxidant capacity and endurance. Eur J ApplPhysiol81: 67–74.
[31]  Ji LL (1999) Antioxidants and oxidative stress in exercise. ExpBiol Med 222: 283–292.
[32]  Leewenburgh C, Hollander J, Leichtweis S, Griffith M, Gore M, et al. (1997) Adaptation of glutathione antioxidant system oxidative-sress markers and delayed onset of muscle fiber specific. Am J Physiol 272: 363–369.
[33]  Child RB, Wilkinson DM, Fallowfield JL (1999) Resting serum antioxidant status is positively correlated with peak oxygen uptake in endurance trained runners. J Sports Med Phys Fitness 39: 282–284.
[34]  Ji LL, Hollander J (2000) Antioxidant defense: effects of aging and exercise. In: Free radical in exercise and aging (Radak Z, Eds). Hum Kin, Champaign II: 35–72.
[35]  Ji LL, Gomez-Cabrera MC, Vina J (2006) Exercise and Hormesis. Activation of cellular antioxidant signaling pathway. Ann N Y AcadSci 1067: 425–435.
[36]  Bloomer RJ, Goldfarb AH (2004) Anaerobic exercise and oxidative stress: a review. Can J ApplPhysiol 29: 245–263.
[37]  Bloomer RJ, Goldfarb AH, Wideman L, McKenzie MJ, Consitt LA (2005) Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 19: 276–285.
[38]  McAnulty SR, McAnulty L, Pascoe DD, Gropper SS, Keith RE, et al. (2005) Hyperthermia increases exercise-induced oxidative stress.Int J Sports Med. 26(3): 188–92.
[39]  Laitano O, Kalsi KK, Pook M, Oliveira AR, González-Alonso J (2010) Separate and combined effects of heat stress and exercise on circulatory markers of oxidative stress in euhydrated humans.Eur J ApplPhysiol. 110(5): 953–60.
[40]  Hillman AR, Vince RV, Taylor L, McNaughton L, Mitchell N, et al. (2011) Exercise-induced dehydration with and without environmental heat stress results in increased oxidative stress. ApplPhysiolNutrMetab 36: 698–706.
[41]  Laitano O, Kalsi KK, Pearson J, Lotlikar M, Reischak-Oliveira A, et al. (2012) Effects of graded exercise-induced dehydration and rehydration on circulatory markers of oxidative stress across the resting and exercising human leg.Eur J ApplPhysiol. 112(5): 1937–44.
[42]  Fehrenbach E, Northoff H (2001) Free radical, exercise, apoptosis and heat shock proteins. ExercImmunolRev 7: 66–89.
[43]  Jethon Z, Murawska-Cia?owicz E, Dzi?giel P, Podhorska-Oko?ów M (2005) Udzia? bia?ek stresowych w adaptacji wysi?kowej. Post BiolKom 32: 697–706.
[44]  Kukkonen-Harjula K, Kauppinen K (2006) Health effects and risks of sauna bathing. Int J Circumpolar Health 65(3): 195–205.
[45]  Gore A, Muralidhar M, Espey MG, Degenhardt K, Mantell LL (2010) Hyperoxia sensing: from molecular mechanisms to significance in disease. J Immunotoxicol 7: 239–254.
[46]  Zinchuk V, Zhadzko D (2012) Sauna effect on blood oxygen transport and prooxidant-antioxidant balance in athletes. Romanian Sports Medicine Society Med Sport 8(3): 1883 –1889.
[47]  Powers SK, Talbert EE, Adhihetty PJ (2011) Reactive oxygen and nitrogen species as intracellular signals in skeletal muscle.J Physiol589. (9): 2129–2138 doi: 10.1113/jphysiol.2010.201327.

Full-Text

comments powered by Disqus