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PLOS Biology  2009 

Turning Meiosis into Mitosis

DOI: 10.1371/journal.pbio.1000124

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Abstract:

Apomixis, or asexual clonal reproduction through seeds, is of immense interest due to its potential application in agriculture. One key element of apomixis is apomeiosis, a deregulation of meiosis that results in a mitotic-like division. We isolated and characterised a novel gene that is directly involved in controlling entry into the second meiotic division. By combining a mutation in this gene with two others that affect key meiotic processes, we created a genotype called MiMe in which meiosis is totally replaced by mitosis. The obtained plants produce functional diploid gametes that are genetically identical to their mother. The creation of the MiMe genotype and apomeiosis phenotype is an important step towards understanding and engineering apomixis.

References

[1]  Bicknell RA, Koltunow AM (2004) Understanding apomixis: recent advances and remaining conundrums. Plant Cell 16: SupplS228–245.
[2]  Koltunow AM, Grossniklaus U (2003) Apomixis: a developmental perspective. Annu Rev Plant Biol 54: 547–574.
[3]  Spillane C, Curtis MD, Grossniklaus U (2004) Apomixis technology development-virgin births in farmers' fields? Nat Biotechnol 22: 687–691.
[4]  Spillane C, Steimer A, Grossniklaus U (2001) Apomixis in agriculture: the quest for clonal seeds. Sex Plant Reprod 14:
[5]  Savidan Y (2001) Transfer of apomixis through wide crosses. In: Savidan Y, Carman J, Dresselhaus T, editors. The flowering of apomixis: From mechanisms to genetic engineering. Mexico: CIMMYT, IRD. pp. 153–167.
[6]  Ozias-Akins P, van Dijk PJ (2007) Mendelian genetics of apomixis in plants. Annu Rev Genet 41: 509–537.
[7]  Gerton JL, Hawley RS (2005) Homologous chromosome interactions in meiosis: diversity amidst conservation. Nat Rev Genet 6: 477–487.
[8]  Grelon M, Vezon D, Gendrot G, Pelletier G (2001) AtSPO11-1 is necessary for efficient meiotic recombination in plants. EMBO J 20: 589–600.
[9]  Chelysheva L, Diallo S, Vezon D, Gendrot G, Vrielynck N, et al. (2005) AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis. J Cell Sci 118: 4621–4632.
[10]  Toufighi K, Brady SM, Austin R, Ly E, Provart NJ (2005) The Botany Array Resource: e-Northerns, Expression Angling, and promoter analyses. Plant J 43: 153–163.
[11]  Schmid M, Davison TS, Henz SR, Pape UJ, Demar M, et al. (2005) A gene expression map of Arabidopsis thaliana development. Nat Genet 37: 501–506.
[12]  d'Erfurth I, Jolivet S, Froger N, Catrice O, Novatchkova M, et al. (2008) Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) lead to the production of diploid pollen grains. PLoS Genet 4: e1000274. doi:10.1371/journal.pgen.1000274.
[13]  Hase Y, Trung KH, Matsunaga T, Tanaka A (2006) A mutation in the uvi4 gene promotes progression of endo-reduplication and confers increased tolerance towards ultraviolet B light. Plant J 46: 317–326.
[14]  Sundaresan V, Springer P, Volpe T, Haward S, Jones JD, et al. (1995) Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev 9: 1797–1810.
[15]  Ito T, Motohashi R, Kuromori T, Mizukado S, Sakurai T, et al. (2002) A new resource of locally transposed Dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome. Plant Physiol 129: 1695–1699.
[16]  Kuromori T, Hirayama T, Kiyosue Y, Takabe H, Mizukado S, et al. (2004) A collection of 11 800 single-copy Ds transposon insertion lines in Arabidopsis. Plant J 37: 897–905.
[17]  Barrell PJ, Grossniklaus U (2005) Confocal microscopy of whole ovules for analysis of reproductive development: the elongate1 mutant affects meiosis II. Plant J 43: 309–320.
[18]  Rhoades MM, Dempsey E (1966) Induction of chromosome doubling at meiosis by the Elongate Gene in maize. Genetics 54: 505–522.
[19]  Pesin JA, Orr-Weaver TL (2008) Regulation of APC/C activators in mitosis and meiosis. Annu Rev Cell Dev Biol 24: 475–499.
[20]  Tyson JJ, Novak B (2008) Temporal organization of the cell cycle. Curr Biol 18: R759–R768.
[21]  Izawa D, Goto M, Yamashita A, Yamano H, Yamamoto M (2005) Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p. Nature 434: 529–533.
[22]  Kimata Y, Trickey M, Izawa D, Gannon J, Yamamoto M, et al. (2008) A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast. Dev Cell 14: 446–454.
[23]  Ohe M, Inoue D, Kanemori Y, Sagata N (2007) Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes. Dev Biol 303: 157–164.
[24]  Tang W, Wu JQ, Guo Y, Hansen DV, Perry JA, et al. (2008) Cdc2 and Mos regulate Emi2 stability to promote the meiosis I-meiosis II transition. Mol Biol Cell 19: 3536–3543.
[25]  Madgwick S, Hansen DV, Levasseur M, Jackson PK, Jones KT (2006) Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesis. J Cell Biol 174: 791–801.
[26]  Ravi M, Marimuthu MP, Siddiqi I (2008) Gamete formation without meiosis in Arabidopsis. Nature 451: 1121–1124.
[27]  Mercier R, Grelon M (2008) Meiosis in plants: ten years of gene discovery. Cytogenet Genome Res 120: 281–290.
[28]  Guitton AE, Berger F (2005) Loss of function of MULTICOPY SUPPRESSOR OF IRA 1 produces nonviable parthenogenetic embryos in Arabidopsis. Curr Biol 15: 750–754.
[29]  Nowack MK, Shirzadi R, Dissmeyer N, Dolf A, Endl E, et al. (2007) Bypassing genomic imprinting allows seed development. Nature 447: 312–315.
[30]  Vignard J, Siwiec T, Chelysheva L, Vrielynck N, Gonord F, et al. (2007) The interplay of RecA-related proteins and the MND1-HOP2 complex during meiosis in Arabidopsis thaliana. PLoS Genet 3: e176. doi:10.1371/journal.pgen.0030176.
[31]  Estelle MA, Somerville C (1987) Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology. Mol Gen Genet 206: 200–206.
[32]  Galtier N, Gouy M, Gautier C (1996) SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12: 543–548.

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