The blood of fresh water, edible fish, Channa punctatus (=murrel) was exposed to sub lethal concentration of triazophos for 24, 48, 72 and 96 h to evaluate alterations in the levels of minerals i.e., sodium, potassium, chloride, calcium, inorganic phosphate, magnesium and iron. The increased chloride mineral along with sodium and potassium play an important role in neuromuscular excitability, acid-base balance and increased osmotic pressure of the fish. The decreased calcium is of great importance in blood coagulation, muscle contraction and nerve transmission while decreased iron is an essential constituent of heame in hemoglobin of the fish. The increased magnesium participates in principal metabolic activities, in formation of bones and teeth. Inorganic phosphate acts as a major buffer and is the basis of energy exchange. Triazophos altered the activities of the macro minerals in C. punctatus during stress periods.