Provision of healthy nutrition, continuous supply with fresh, quality and price acceptable food, along with the intensive increase in the number of citydwellers, shows that the planning and construction of fresh food wholesale markets is necessary. Modern wholesale markets are technological and commercial complexes with considerable size of built-up areas, roads and extensive infrastructure. Large number of factors affects the planning and design of wholesale markets. Important factor affecting the spatial and functional solution for fruit and vegetable promotion and sale facilities buildings, is ambiental condition requirements. Ambiental condition requirements include temperature and humidity, as well as hygienic and health requirements. Regulations and guidelines that exist in the agri-food system supply chain 'from farm to fork' are also valid for fresh food wholesale markets. The organizations that provide recommendations are Food Agriculture Organisation (FAO), World Health Organisation (WHO), and World Union of Wholesale Markets (WUWM). Respect of the cold chain, traceability and hygiene and health standards are recommended in their Guidelines. These recommendations are to be implemented in the national regulations. This research assumption is that changes in ambiental conditions, caused by regulations and guidelines, significantly contributed to the transformation of spatial and functional wholesale fruit and vegetables building solutions. By those regulations and guidelines and new technology solutions this spatial and functional transformation has been accelerated The paper also presents a comparative analysis of spatial and functional solutions by the basic parameters such as facility size, functional layout, type and size of premises. Spatial-functional solutions of the buildings in wholesale market in Halles-e in Paris, Rome and the CAR-Corbas Lyon in Lyon were analysed. The research results confirmed the assumptions that changes in ambiental conditions entail changes in the spatialfunctional solutions i.e. change of the complete geometry of buildings and premises. The results have important application for practical use in planning and design of modern fresh fruit and vegetable market buildings.