This study investigated the presence of pathological gambling characteristics in a sample of university students. In total, 908 students from different faculties at the University of Murcia (Spain) were assessed by answering the South Oaks Gambling Screen (SOGS). The results showed that 4.18% were probable pathological gamblers, 6.39% were at risk of pathological gambling, 49.5% could gamble without such risk, and 39.91% stated they never gambled. There were more male than female pathological gamblers and the male gamblers played more kinds of games than female gamblers. Male gamblers also spent more money on gambling per day than women and experienced more criticism for doing so than women.